Novel and cross-species microsatellite markers for parentage analysis in Sanderling Calidris alba

• Published in: Journal of Ornithology
• Main Authors: Luttikhuizen, Pieternella C., Bol, Anneke, Witte, Harry, van Bleijswijk, Judith, Haddrath, Oliver, Baker, Allan J., Piersma, Theunis, Reneerkens, Jeroen
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2011
• Subjects: Microsatellite markers; Parentage analysis; Breeding system; Population genetics; INTEGRATED SOFTWARE; COMPUTER-PROGRAM; POPULATION; Greenland; Calidris alba; Sanderling
• Online Access: https://hdl.handle.net/11370/9d227d65-c0ff-452d-8334-aa6aecbde362; https://research.rug.nl/en/publications/9d227d65-c0ff-452d-8334-aa6aecbde362; https://doi.org/10.1007/s10336-011-0681-6

We isolated and tested six novel microsatellite loci in Sanderling (Calidris alba) from Greenland for paternity analyses. In addition, we tested 11 already published microsatellite markers which were originally developed for the congeneric species, the Pectoral Sandpiper (C. melanotos). All loci were polymorphic, but five of the cross-species loci were not scorable due to suboptimal amplification patterns. The 12 successful loci were tested on 87 individuals, yielding an average of 9.0 (range 4-19) alleles per locus and mean expected heterozygosity of 0.70. Because this dataset contained families, tests for Hardy-Weinberg equilibrium, linkage disequilibrium and probability of identity were done on a subset of the data containing 25 adults caught in the same year. The overall probability of identity was 1.0 x 10(-13). Only one locus displayed significant homozygote excess and all loci were unlinked. On the basis of female heterozygotes, all loci are assumed to be autosomal.