Springtime dynamics, productivity and activity of prokaryotes in two Arctic fjords
In the Kongsfjorden-Krossfjorden system (Spitsbergen), increasing temperatures enhance glacier melting and concomitant intrusion of freshwater. These altered conditions affect the timing, intensity, and composition of the phytoplankton spring bloom in Kongsfjorden; yet, the effects on prokaryotes (b...
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Format: | Article in Journal/Newspaper |
Language: | English |
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2016
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Online Access: | https://hdl.handle.net/11370/685a0f90-96b6-4376-beb5-ea103cdb97a5 https://research.rug.nl/en/publications/685a0f90-96b6-4376-beb5-ea103cdb97a5 https://doi.org/10.1007/s00300-015-1866-x https://pure.rug.nl/ws/files/62000536/Springtime_dynamics_productivity_and_activity_of_prokaryotes_in_two_Arctic_fjords.pdf http://www.scopus.com/inward/record.url?scp=84952658804&partnerID=8YFLogxK |
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ftunigroningenpu:oai:pure.rug.nl:publications/685a0f90-96b6-4376-beb5-ea103cdb97a5 2024-06-23T07:48:38+00:00 Springtime dynamics, productivity and activity of prokaryotes in two Arctic fjords Piquet, A. M. -T. Maat, D. S. Confurius-Guns, V. Sintes, E. Herndl, G. J. van de Poll, W. H. Wiencke, C. Buma, A. G. J. Bolhuis, H. 2016-10 application/pdf https://hdl.handle.net/11370/685a0f90-96b6-4376-beb5-ea103cdb97a5 https://research.rug.nl/en/publications/685a0f90-96b6-4376-beb5-ea103cdb97a5 https://doi.org/10.1007/s00300-015-1866-x https://pure.rug.nl/ws/files/62000536/Springtime_dynamics_productivity_and_activity_of_prokaryotes_in_two_Arctic_fjords.pdf http://www.scopus.com/inward/record.url?scp=84952658804&partnerID=8YFLogxK eng eng https://research.rug.nl/en/publications/685a0f90-96b6-4376-beb5-ea103cdb97a5 info:eu-repo/semantics/openAccess Piquet , A M -T , Maat , D S , Confurius-Guns , V , Sintes , E , Herndl , G J , van de Poll , W H , Wiencke , C , Buma , A G J & Bolhuis , H 2016 , ' Springtime dynamics, productivity and activity of prokaryotes in two Arctic fjords ' , Polar Biology , vol. 39 , no. 10 , pp. 1749-1763 . https://doi.org/10.1007/s00300-015-1866-x Polar Spitsbergen Bacteria Archaea Glacier melting Spring bloom Bacterial production Micro-CARD-FISH 16S RIBOSOMAL-RNA IN-SITU HYBRIDIZATION CATALYZED REPORTER DEPOSITION ARCHAEAL COMMUNITIES BACTERIAL GROUPS MARINE-BACTERIA SEA-ICE OLIGONUCLEOTIDE PROBES MICROBIAL COMMUNITIES PELAGIC BACTERIA article 2016 ftunigroningenpu https://doi.org/10.1007/s00300-015-1866-x 2024-06-10T16:22:58Z In the Kongsfjorden-Krossfjorden system (Spitsbergen), increasing temperatures enhance glacier melting and concomitant intrusion of freshwater. These altered conditions affect the timing, intensity, and composition of the phytoplankton spring bloom in Kongsfjorden; yet, the effects on prokaryotes (bacteria and archaea) are not well understood. The aim of this study was to examine springtime prokaryote communities in both fjords as a function of hydrographic and phytoplankton variability. Prokaryote community composition was studied in two consecutive years by molecular fingerprinting of the 16S rRNA gene. In addition, we measured bacterial abundance, productivity (H-3-Leucine uptake), and single-cell activity using catalyzed reporter deposition fluorescence in situ hybridization combined with microautoradiography. Differences in bacterial and archaeal communities were found between Kongsfjorden and Krossfjorden. Furthermore, an increase in productivity, abundance, and proportion of active bacterial cells was observed during the course of spring. Bacteroidetes were the most abundant bacterial group among the assessed taxa in both Kongsfjorden and Krossfjorden. Multivariate analysis of the microbial community fingerprints revealed a strong temporal shaping of both the bacterial and archaeal communities in addition to a spatial separation between the two fjords. A significant part of the observed bacterial variation could be explained by cyanobacterial biomass, as deduced from pigment analysis, and by phosphate concentration. Archaea were mainly controlled by abiotic factors. We speculate that the bacterial response to hydrographic changes and glacier meltwater is mediated through shifts in phytoplankton abundance and composition, whereas archaea are directly influenced by abiotic environmental variables. Article in Journal/Newspaper Arctic Arctic Kongsfjord* Kongsfjorden Krossfjord* Phytoplankton Polar Biology Sea ice Spitsbergen University of Groningen research database Arctic Krossfjorden ENVELOPE(11.742,11.742,79.141,79.141) Polar Biology 39 10 1749 1763 |
institution |
Open Polar |
collection |
University of Groningen research database |
op_collection_id |
ftunigroningenpu |
language |
English |
topic |
Polar Spitsbergen Bacteria Archaea Glacier melting Spring bloom Bacterial production Micro-CARD-FISH 16S RIBOSOMAL-RNA IN-SITU HYBRIDIZATION CATALYZED REPORTER DEPOSITION ARCHAEAL COMMUNITIES BACTERIAL GROUPS MARINE-BACTERIA SEA-ICE OLIGONUCLEOTIDE PROBES MICROBIAL COMMUNITIES PELAGIC BACTERIA |
spellingShingle |
Polar Spitsbergen Bacteria Archaea Glacier melting Spring bloom Bacterial production Micro-CARD-FISH 16S RIBOSOMAL-RNA IN-SITU HYBRIDIZATION CATALYZED REPORTER DEPOSITION ARCHAEAL COMMUNITIES BACTERIAL GROUPS MARINE-BACTERIA SEA-ICE OLIGONUCLEOTIDE PROBES MICROBIAL COMMUNITIES PELAGIC BACTERIA Piquet, A. M. -T. Maat, D. S. Confurius-Guns, V. Sintes, E. Herndl, G. J. van de Poll, W. H. Wiencke, C. Buma, A. G. J. Bolhuis, H. Springtime dynamics, productivity and activity of prokaryotes in two Arctic fjords |
topic_facet |
Polar Spitsbergen Bacteria Archaea Glacier melting Spring bloom Bacterial production Micro-CARD-FISH 16S RIBOSOMAL-RNA IN-SITU HYBRIDIZATION CATALYZED REPORTER DEPOSITION ARCHAEAL COMMUNITIES BACTERIAL GROUPS MARINE-BACTERIA SEA-ICE OLIGONUCLEOTIDE PROBES MICROBIAL COMMUNITIES PELAGIC BACTERIA |
description |
In the Kongsfjorden-Krossfjorden system (Spitsbergen), increasing temperatures enhance glacier melting and concomitant intrusion of freshwater. These altered conditions affect the timing, intensity, and composition of the phytoplankton spring bloom in Kongsfjorden; yet, the effects on prokaryotes (bacteria and archaea) are not well understood. The aim of this study was to examine springtime prokaryote communities in both fjords as a function of hydrographic and phytoplankton variability. Prokaryote community composition was studied in two consecutive years by molecular fingerprinting of the 16S rRNA gene. In addition, we measured bacterial abundance, productivity (H-3-Leucine uptake), and single-cell activity using catalyzed reporter deposition fluorescence in situ hybridization combined with microautoradiography. Differences in bacterial and archaeal communities were found between Kongsfjorden and Krossfjorden. Furthermore, an increase in productivity, abundance, and proportion of active bacterial cells was observed during the course of spring. Bacteroidetes were the most abundant bacterial group among the assessed taxa in both Kongsfjorden and Krossfjorden. Multivariate analysis of the microbial community fingerprints revealed a strong temporal shaping of both the bacterial and archaeal communities in addition to a spatial separation between the two fjords. A significant part of the observed bacterial variation could be explained by cyanobacterial biomass, as deduced from pigment analysis, and by phosphate concentration. Archaea were mainly controlled by abiotic factors. We speculate that the bacterial response to hydrographic changes and glacier meltwater is mediated through shifts in phytoplankton abundance and composition, whereas archaea are directly influenced by abiotic environmental variables. |
format |
Article in Journal/Newspaper |
author |
Piquet, A. M. -T. Maat, D. S. Confurius-Guns, V. Sintes, E. Herndl, G. J. van de Poll, W. H. Wiencke, C. Buma, A. G. J. Bolhuis, H. |
author_facet |
Piquet, A. M. -T. Maat, D. S. Confurius-Guns, V. Sintes, E. Herndl, G. J. van de Poll, W. H. Wiencke, C. Buma, A. G. J. Bolhuis, H. |
author_sort |
Piquet, A. M. -T. |
title |
Springtime dynamics, productivity and activity of prokaryotes in two Arctic fjords |
title_short |
Springtime dynamics, productivity and activity of prokaryotes in two Arctic fjords |
title_full |
Springtime dynamics, productivity and activity of prokaryotes in two Arctic fjords |
title_fullStr |
Springtime dynamics, productivity and activity of prokaryotes in two Arctic fjords |
title_full_unstemmed |
Springtime dynamics, productivity and activity of prokaryotes in two Arctic fjords |
title_sort |
springtime dynamics, productivity and activity of prokaryotes in two arctic fjords |
publishDate |
2016 |
url |
https://hdl.handle.net/11370/685a0f90-96b6-4376-beb5-ea103cdb97a5 https://research.rug.nl/en/publications/685a0f90-96b6-4376-beb5-ea103cdb97a5 https://doi.org/10.1007/s00300-015-1866-x https://pure.rug.nl/ws/files/62000536/Springtime_dynamics_productivity_and_activity_of_prokaryotes_in_two_Arctic_fjords.pdf http://www.scopus.com/inward/record.url?scp=84952658804&partnerID=8YFLogxK |
long_lat |
ENVELOPE(11.742,11.742,79.141,79.141) |
geographic |
Arctic Krossfjorden |
geographic_facet |
Arctic Krossfjorden |
genre |
Arctic Arctic Kongsfjord* Kongsfjorden Krossfjord* Phytoplankton Polar Biology Sea ice Spitsbergen |
genre_facet |
Arctic Arctic Kongsfjord* Kongsfjorden Krossfjord* Phytoplankton Polar Biology Sea ice Spitsbergen |
op_source |
Piquet , A M -T , Maat , D S , Confurius-Guns , V , Sintes , E , Herndl , G J , van de Poll , W H , Wiencke , C , Buma , A G J & Bolhuis , H 2016 , ' Springtime dynamics, productivity and activity of prokaryotes in two Arctic fjords ' , Polar Biology , vol. 39 , no. 10 , pp. 1749-1763 . https://doi.org/10.1007/s00300-015-1866-x |
op_relation |
https://research.rug.nl/en/publications/685a0f90-96b6-4376-beb5-ea103cdb97a5 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s00300-015-1866-x |
container_title |
Polar Biology |
container_volume |
39 |
container_issue |
10 |
container_start_page |
1749 |
op_container_end_page |
1763 |
_version_ |
1802638983602110464 |