Assessment of the amount of body water in the Red Knot (Calidris canutus):An evaluation of the principle of isotope dilution with 2H, 17O, and 18O as easured with laser spectrometry and isotope ratio mass spectrometry
We have used the isotope dilution technique to study changes in the body composition of a migratory shorebird species (Red Knot, Calidris canutus) through an assessment of the amount of body water in it. Birds were quantitatively injected with a dose of water with elevated concentrations of H-2, O-1...
Published in: | Isotopes in Environmental and Health Studies |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2006
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Online Access: | https://hdl.handle.net/11370/499cf286-54d0-4df7-82d1-9613b9d89895 https://research.rug.nl/en/publications/499cf286-54d0-4df7-82d1-9613b9d89895 https://doi.org/10.1080/10256010500503197 https://pure.rug.nl/ws/files/13344761/2006IsotEnvironHealthStudKerstelCorr.pdf https://pure.rug.nl/ws/files/13344762/2006IsotEnvironHealthStudKerstel.pdf |
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ftunigroningenpu:oai:pure.rug.nl:publications/499cf286-54d0-4df7-82d1-9613b9d89895 2024-06-02T08:04:47+00:00 Assessment of the amount of body water in the Red Knot (Calidris canutus):An evaluation of the principle of isotope dilution with 2H, 17O, and 18O as easured with laser spectrometry and isotope ratio mass spectrometry Kerstel, Erik R.T. Piersma, Theunis A.J. Gessaman, G. Jim Dekinga, Anne Meijer, Harro A.J. Visser, G. Henk 2006-03 application/pdf https://hdl.handle.net/11370/499cf286-54d0-4df7-82d1-9613b9d89895 https://research.rug.nl/en/publications/499cf286-54d0-4df7-82d1-9613b9d89895 https://doi.org/10.1080/10256010500503197 https://pure.rug.nl/ws/files/13344761/2006IsotEnvironHealthStudKerstelCorr.pdf https://pure.rug.nl/ws/files/13344762/2006IsotEnvironHealthStudKerstel.pdf eng eng https://research.rug.nl/en/publications/499cf286-54d0-4df7-82d1-9613b9d89895 info:eu-repo/semantics/openAccess Kerstel , E R T , Piersma , T A J , Gessaman , G J , Dekinga , A , Meijer , H A J & Visser , G H 2006 , ' Assessment of the amount of body water in the Red Knot (Calidris canutus) : An evaluation of the principle of isotope dilution with 2H, 17O, and 18O as easured with laser spectrometry and isotope ratio mass spectrometry ' , Isotopes in Environmental and Health Studies , vol. 42 , no. 1 , pp. 1-7 . https://doi.org/10.1080/10256010500503197 birds body water hydrogen-2 isotope dilution IR spectroscopy IRMS oxygen-17 oxygen-18 LABELED WATER HYDROGEN article 2006 ftunigroningenpu https://doi.org/10.1080/10256010500503197 2024-05-07T17:51:13Z We have used the isotope dilution technique to study changes in the body composition of a migratory shorebird species (Red Knot, Calidris canutus) through an assessment of the amount of body water in it. Birds were quantitatively injected with a dose of water with elevated concentrations of H-2, O-17, and O-18. Thereafter, blood samples were taken and distilled. The resulting water samples were analysed using an isotope ratio mass spectrometry (for H-2 and O-18 only) and a stable isotope ratio infrared laser spectrometry (H-2, O-17, and O-18) to yield estimates of the amount of body water in the birds, which in turn could be correlated to the amount of body fat. Here, we validate laser spectrometry against mass spectrometry and show that all three isotopes may be used for body water determinations. This opens the way to the extension of the doubly labelled water method, used for the determination of energy expenditure, to a triply labelled water method, incorporating an evaporative water loss correction on a subject-by-subject basis or, alternatively, the reduction of the analytical errors by statistically combining the O-17 and O-18 measurements. Article in Journal/Newspaper Calidris canutus Red Knot University of Groningen research database Isotopes in Environmental and Health Studies 42 1 1 7 |
institution |
Open Polar |
collection |
University of Groningen research database |
op_collection_id |
ftunigroningenpu |
language |
English |
topic |
birds body water hydrogen-2 isotope dilution IR spectroscopy IRMS oxygen-17 oxygen-18 LABELED WATER HYDROGEN |
spellingShingle |
birds body water hydrogen-2 isotope dilution IR spectroscopy IRMS oxygen-17 oxygen-18 LABELED WATER HYDROGEN Kerstel, Erik R.T. Piersma, Theunis A.J. Gessaman, G. Jim Dekinga, Anne Meijer, Harro A.J. Visser, G. Henk Assessment of the amount of body water in the Red Knot (Calidris canutus):An evaluation of the principle of isotope dilution with 2H, 17O, and 18O as easured with laser spectrometry and isotope ratio mass spectrometry |
topic_facet |
birds body water hydrogen-2 isotope dilution IR spectroscopy IRMS oxygen-17 oxygen-18 LABELED WATER HYDROGEN |
description |
We have used the isotope dilution technique to study changes in the body composition of a migratory shorebird species (Red Knot, Calidris canutus) through an assessment of the amount of body water in it. Birds were quantitatively injected with a dose of water with elevated concentrations of H-2, O-17, and O-18. Thereafter, blood samples were taken and distilled. The resulting water samples were analysed using an isotope ratio mass spectrometry (for H-2 and O-18 only) and a stable isotope ratio infrared laser spectrometry (H-2, O-17, and O-18) to yield estimates of the amount of body water in the birds, which in turn could be correlated to the amount of body fat. Here, we validate laser spectrometry against mass spectrometry and show that all three isotopes may be used for body water determinations. This opens the way to the extension of the doubly labelled water method, used for the determination of energy expenditure, to a triply labelled water method, incorporating an evaporative water loss correction on a subject-by-subject basis or, alternatively, the reduction of the analytical errors by statistically combining the O-17 and O-18 measurements. |
format |
Article in Journal/Newspaper |
author |
Kerstel, Erik R.T. Piersma, Theunis A.J. Gessaman, G. Jim Dekinga, Anne Meijer, Harro A.J. Visser, G. Henk |
author_facet |
Kerstel, Erik R.T. Piersma, Theunis A.J. Gessaman, G. Jim Dekinga, Anne Meijer, Harro A.J. Visser, G. Henk |
author_sort |
Kerstel, Erik R.T. |
title |
Assessment of the amount of body water in the Red Knot (Calidris canutus):An evaluation of the principle of isotope dilution with 2H, 17O, and 18O as easured with laser spectrometry and isotope ratio mass spectrometry |
title_short |
Assessment of the amount of body water in the Red Knot (Calidris canutus):An evaluation of the principle of isotope dilution with 2H, 17O, and 18O as easured with laser spectrometry and isotope ratio mass spectrometry |
title_full |
Assessment of the amount of body water in the Red Knot (Calidris canutus):An evaluation of the principle of isotope dilution with 2H, 17O, and 18O as easured with laser spectrometry and isotope ratio mass spectrometry |
title_fullStr |
Assessment of the amount of body water in the Red Knot (Calidris canutus):An evaluation of the principle of isotope dilution with 2H, 17O, and 18O as easured with laser spectrometry and isotope ratio mass spectrometry |
title_full_unstemmed |
Assessment of the amount of body water in the Red Knot (Calidris canutus):An evaluation of the principle of isotope dilution with 2H, 17O, and 18O as easured with laser spectrometry and isotope ratio mass spectrometry |
title_sort |
assessment of the amount of body water in the red knot (calidris canutus):an evaluation of the principle of isotope dilution with 2h, 17o, and 18o as easured with laser spectrometry and isotope ratio mass spectrometry |
publishDate |
2006 |
url |
https://hdl.handle.net/11370/499cf286-54d0-4df7-82d1-9613b9d89895 https://research.rug.nl/en/publications/499cf286-54d0-4df7-82d1-9613b9d89895 https://doi.org/10.1080/10256010500503197 https://pure.rug.nl/ws/files/13344761/2006IsotEnvironHealthStudKerstelCorr.pdf https://pure.rug.nl/ws/files/13344762/2006IsotEnvironHealthStudKerstel.pdf |
genre |
Calidris canutus Red Knot |
genre_facet |
Calidris canutus Red Knot |
op_source |
Kerstel , E R T , Piersma , T A J , Gessaman , G J , Dekinga , A , Meijer , H A J & Visser , G H 2006 , ' Assessment of the amount of body water in the Red Knot (Calidris canutus) : An evaluation of the principle of isotope dilution with 2H, 17O, and 18O as easured with laser spectrometry and isotope ratio mass spectrometry ' , Isotopes in Environmental and Health Studies , vol. 42 , no. 1 , pp. 1-7 . https://doi.org/10.1080/10256010500503197 |
op_relation |
https://research.rug.nl/en/publications/499cf286-54d0-4df7-82d1-9613b9d89895 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1080/10256010500503197 |
container_title |
Isotopes in Environmental and Health Studies |
container_volume |
42 |
container_issue |
1 |
container_start_page |
1 |
op_container_end_page |
7 |
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1800749441891696640 |