Molecular vs. phenotypic sexing in Red Knots
Using a reference collection of blood samples of Red Knots (Calidris canutus) from Florida and Alaska whose sex was determined by dissection, we demonstrate that the molecular method of sexing based on the size of polymerase chain reaction (PCR) products from the CHD genes after digestion with Hae I...
Published in: | The Condor |
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Language: | English |
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1999
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Online Access: | https://hdl.handle.net/11370/35e84bea-180f-402a-9875-ddceb3ee086f https://research.rug.nl/en/publications/35e84bea-180f-402a-9875-ddceb3ee086f https://doi.org/10.2307/1370083 https://pure.rug.nl/ws/files/6659024/1999CondorBaker.pdf |
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ftunigroningenpu:oai:pure.rug.nl:publications/35e84bea-180f-402a-9875-ddceb3ee086f 2024-06-23T07:51:55+00:00 Molecular vs. phenotypic sexing in Red Knots Baker, Allan J. Piersma, Theunis Greenslade, Annette D. 1999-11 application/pdf https://hdl.handle.net/11370/35e84bea-180f-402a-9875-ddceb3ee086f https://research.rug.nl/en/publications/35e84bea-180f-402a-9875-ddceb3ee086f https://doi.org/10.2307/1370083 https://pure.rug.nl/ws/files/6659024/1999CondorBaker.pdf eng eng https://research.rug.nl/en/publications/35e84bea-180f-402a-9875-ddceb3ee086f info:eu-repo/semantics/openAccess Baker , A J , Piersma , T & Greenslade , A D 1999 , ' Molecular vs. phenotypic sexing in Red Knots ' , Condor , vol. 101 , no. 4 , pp. 887-893 . https://doi.org/10.2307/1370083 Calidris canutus CHD genes molecular sexing morphometrics plumage Red Knots shorebirds BIRDS IDENTIFICATION MIGRATION GENE article 1999 ftunigroningenpu https://doi.org/10.2307/1370083 2024-06-03T16:11:09Z Using a reference collection of blood samples of Red Knots (Calidris canutus) from Florida and Alaska whose sex was determined by dissection, we demonstrate that the molecular method of sexing based on the size of polymerase chain reaction (PCR) products from the CHD genes after digestion with Hae III accurately identifies gender in this species. In males, the 110 basepair PCR product is cleaved into two smaller fragments, whereas in females this fragment remains uncut. Molecular sexing of other samples of Red Knots from the Dutch Wadden Sea, southern Brazil, and Delaware Bay revealed that methods using a discriminant function on size or plumage dimorphism are only partly effective in correctly assigning sex of birds. Given the low cost and accuracy of molecular sexing on very small blood samples, we anticipate that it will find increasing use in studies of the evolutionary ecology of shorebirds and other avian species. Article in Journal/Newspaper Calidris canutus Alaska University of Groningen research database The Condor 101 4 887 893 |
institution |
Open Polar |
collection |
University of Groningen research database |
op_collection_id |
ftunigroningenpu |
language |
English |
topic |
Calidris canutus CHD genes molecular sexing morphometrics plumage Red Knots shorebirds BIRDS IDENTIFICATION MIGRATION GENE |
spellingShingle |
Calidris canutus CHD genes molecular sexing morphometrics plumage Red Knots shorebirds BIRDS IDENTIFICATION MIGRATION GENE Baker, Allan J. Piersma, Theunis Greenslade, Annette D. Molecular vs. phenotypic sexing in Red Knots |
topic_facet |
Calidris canutus CHD genes molecular sexing morphometrics plumage Red Knots shorebirds BIRDS IDENTIFICATION MIGRATION GENE |
description |
Using a reference collection of blood samples of Red Knots (Calidris canutus) from Florida and Alaska whose sex was determined by dissection, we demonstrate that the molecular method of sexing based on the size of polymerase chain reaction (PCR) products from the CHD genes after digestion with Hae III accurately identifies gender in this species. In males, the 110 basepair PCR product is cleaved into two smaller fragments, whereas in females this fragment remains uncut. Molecular sexing of other samples of Red Knots from the Dutch Wadden Sea, southern Brazil, and Delaware Bay revealed that methods using a discriminant function on size or plumage dimorphism are only partly effective in correctly assigning sex of birds. Given the low cost and accuracy of molecular sexing on very small blood samples, we anticipate that it will find increasing use in studies of the evolutionary ecology of shorebirds and other avian species. |
format |
Article in Journal/Newspaper |
author |
Baker, Allan J. Piersma, Theunis Greenslade, Annette D. |
author_facet |
Baker, Allan J. Piersma, Theunis Greenslade, Annette D. |
author_sort |
Baker, Allan J. |
title |
Molecular vs. phenotypic sexing in Red Knots |
title_short |
Molecular vs. phenotypic sexing in Red Knots |
title_full |
Molecular vs. phenotypic sexing in Red Knots |
title_fullStr |
Molecular vs. phenotypic sexing in Red Knots |
title_full_unstemmed |
Molecular vs. phenotypic sexing in Red Knots |
title_sort |
molecular vs. phenotypic sexing in red knots |
publishDate |
1999 |
url |
https://hdl.handle.net/11370/35e84bea-180f-402a-9875-ddceb3ee086f https://research.rug.nl/en/publications/35e84bea-180f-402a-9875-ddceb3ee086f https://doi.org/10.2307/1370083 https://pure.rug.nl/ws/files/6659024/1999CondorBaker.pdf |
genre |
Calidris canutus Alaska |
genre_facet |
Calidris canutus Alaska |
op_source |
Baker , A J , Piersma , T & Greenslade , A D 1999 , ' Molecular vs. phenotypic sexing in Red Knots ' , Condor , vol. 101 , no. 4 , pp. 887-893 . https://doi.org/10.2307/1370083 |
op_relation |
https://research.rug.nl/en/publications/35e84bea-180f-402a-9875-ddceb3ee086f |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.2307/1370083 |
container_title |
The Condor |
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101 |
container_issue |
4 |
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887 |
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893 |
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1802643067789901824 |