Bacterial gene expression at low temperatures
Under suboptimal environmental conditions such as low temperatures, many bacteria have an extended lag phase, altered cell structures, and composition such as a less fluid (more rigid) and leaky cytoplasmic membrane. As a result, cells may die, enter into a starvation mode of metabolism or a physiol...
Published in: | Extremophiles |
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Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2012
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Subjects: | |
Online Access: | https://hdl.handle.net/11370/1ff9ee5c-af65-4b69-883a-286157b6beec https://research.rug.nl/en/publications/1ff9ee5c-af65-4b69-883a-286157b6beec https://doi.org/10.1007/s00792-011-0423-y |
Summary: | Under suboptimal environmental conditions such as low temperatures, many bacteria have an extended lag phase, altered cell structures, and composition such as a less fluid (more rigid) and leaky cytoplasmic membrane. As a result, cells may die, enter into a starvation mode of metabolism or a physiologically viable but non-culturable (VBNC) state. In the latter state, the amount of gene expression per cell is virtually undetectable. In this article, gene expression under (suboptimal) low temperature conditions in non-psychrophilic environmental bacteria is examined. The pros and cons of some of the molecular methodologies for gene expression analysis are also discussed. |
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