Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches
P>1. Understanding ecological phenomena often requires an accurate assessment of the timing of events. To estimate the time since a diet shift in animals without knowledge on the isotope ratios of either the old or the new diet, isotope ratio measurements in two different tissues (e.g. blood plas...
Published in: | Functional Ecology |
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2010
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Online Access: | https://hdl.handle.net/11370/1f8b304b-c294-4e57-94d0-49421327449d https://research.rug.nl/en/publications/1f8b304b-c294-4e57-94d0-49421327449d https://doi.org/10.1111/j.1365-2435.2010.01689.x |
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ftunigroningenpu:oai:pure.rug.nl:publications/1f8b304b-c294-4e57-94d0-49421327449d 2024-06-23T07:51:56+00:00 Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches Klaassen, Marcel Piersma, Theunis Korthals, Harry Dekinga, Anne Dietz, Maurine W. 2010-08 https://hdl.handle.net/11370/1f8b304b-c294-4e57-94d0-49421327449d https://research.rug.nl/en/publications/1f8b304b-c294-4e57-94d0-49421327449d https://doi.org/10.1111/j.1365-2435.2010.01689.x eng eng https://research.rug.nl/en/publications/1f8b304b-c294-4e57-94d0-49421327449d info:eu-repo/semantics/closedAccess Klaassen , M , Piersma , T , Korthals , H , Dekinga , A & Dietz , M W 2010 , ' Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches ' , Functional Ecology , vol. 24 , no. 4 , pp. 796-804 . https://doi.org/10.1111/j.1365-2435.2010.01689.x isotopic clock mathematical model sensitivity analysis stable carbon isotope timing of events CLIMATE-CHANGE N-15 INCORPORATION STABLE-ISOTOPES CARBON ISOTOPES ANIMAL-TISSUES TURNOVER C-13 REPRODUCTION DELTA-C-13 NITROGEN article 2010 ftunigroningenpu https://doi.org/10.1111/j.1365-2435.2010.01689.x 2024-06-10T16:06:18Z P>1. Understanding ecological phenomena often requires an accurate assessment of the timing of events. To estimate the time since a diet shift in animals without knowledge on the isotope ratios of either the old or the new diet, isotope ratio measurements in two different tissues (e.g. blood plasma and blood cells) at a single point in time can be used. For this 'isotopic-clock' principle, we present here a mathematical model that yields an analytical and easily calculated outcome. 2. Compared with a previously published model, our model assumes the isotopic difference between the old and new diets to be constant if multiple measurements are taken on the same subject at different points in time. Furthermore, to estimate the time since diet switch, no knowledge of the isotopic signature of tissues under the old diet, but only under the new diet is required. 3. The two models are compared using three calibration data sets including a novel one based on a diet shift experiment in a shorebird (red knot Calidris canutus); sensitivity analyses were conducted. The two models behaved differently and each may prove rather unsatisfactory depending on the system under investigation. A single-tissue model, requiring knowledge of both the old and new diets, generally behaved quite reliably. 4. As blood (cells) and plasma are particularly useful tissues for isotopic-clock research, we trawled the literature on turnover rates in whole blood, cells and plasma. Unfortunately, turnover rate predictions using allometric relations are too unreliable to be used directly in isotopic-clock calculations. 5. We advocate that before applying the isotopic-clock methodology, the propagation of error in the 'time-since-diet-shift' estimation is carefully assessed for the system under scrutiny using a sensitivity analysis as proposed here. Article in Journal/Newspaper Calidris canutus Red Knot University of Groningen research database Functional Ecology 24 4 796 804 |
institution |
Open Polar |
collection |
University of Groningen research database |
op_collection_id |
ftunigroningenpu |
language |
English |
topic |
isotopic clock mathematical model sensitivity analysis stable carbon isotope timing of events CLIMATE-CHANGE N-15 INCORPORATION STABLE-ISOTOPES CARBON ISOTOPES ANIMAL-TISSUES TURNOVER C-13 REPRODUCTION DELTA-C-13 NITROGEN |
spellingShingle |
isotopic clock mathematical model sensitivity analysis stable carbon isotope timing of events CLIMATE-CHANGE N-15 INCORPORATION STABLE-ISOTOPES CARBON ISOTOPES ANIMAL-TISSUES TURNOVER C-13 REPRODUCTION DELTA-C-13 NITROGEN Klaassen, Marcel Piersma, Theunis Korthals, Harry Dekinga, Anne Dietz, Maurine W. Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
topic_facet |
isotopic clock mathematical model sensitivity analysis stable carbon isotope timing of events CLIMATE-CHANGE N-15 INCORPORATION STABLE-ISOTOPES CARBON ISOTOPES ANIMAL-TISSUES TURNOVER C-13 REPRODUCTION DELTA-C-13 NITROGEN |
description |
P>1. Understanding ecological phenomena often requires an accurate assessment of the timing of events. To estimate the time since a diet shift in animals without knowledge on the isotope ratios of either the old or the new diet, isotope ratio measurements in two different tissues (e.g. blood plasma and blood cells) at a single point in time can be used. For this 'isotopic-clock' principle, we present here a mathematical model that yields an analytical and easily calculated outcome. 2. Compared with a previously published model, our model assumes the isotopic difference between the old and new diets to be constant if multiple measurements are taken on the same subject at different points in time. Furthermore, to estimate the time since diet switch, no knowledge of the isotopic signature of tissues under the old diet, but only under the new diet is required. 3. The two models are compared using three calibration data sets including a novel one based on a diet shift experiment in a shorebird (red knot Calidris canutus); sensitivity analyses were conducted. The two models behaved differently and each may prove rather unsatisfactory depending on the system under investigation. A single-tissue model, requiring knowledge of both the old and new diets, generally behaved quite reliably. 4. As blood (cells) and plasma are particularly useful tissues for isotopic-clock research, we trawled the literature on turnover rates in whole blood, cells and plasma. Unfortunately, turnover rate predictions using allometric relations are too unreliable to be used directly in isotopic-clock calculations. 5. We advocate that before applying the isotopic-clock methodology, the propagation of error in the 'time-since-diet-shift' estimation is carefully assessed for the system under scrutiny using a sensitivity analysis as proposed here. |
format |
Article in Journal/Newspaper |
author |
Klaassen, Marcel Piersma, Theunis Korthals, Harry Dekinga, Anne Dietz, Maurine W. |
author_facet |
Klaassen, Marcel Piersma, Theunis Korthals, Harry Dekinga, Anne Dietz, Maurine W. |
author_sort |
Klaassen, Marcel |
title |
Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
title_short |
Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
title_full |
Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
title_fullStr |
Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
title_full_unstemmed |
Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
title_sort |
single-point isotope measurements in blood cells and plasma to estimate the time since diet switches |
publishDate |
2010 |
url |
https://hdl.handle.net/11370/1f8b304b-c294-4e57-94d0-49421327449d https://research.rug.nl/en/publications/1f8b304b-c294-4e57-94d0-49421327449d https://doi.org/10.1111/j.1365-2435.2010.01689.x |
genre |
Calidris canutus Red Knot |
genre_facet |
Calidris canutus Red Knot |
op_source |
Klaassen , M , Piersma , T , Korthals , H , Dekinga , A & Dietz , M W 2010 , ' Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches ' , Functional Ecology , vol. 24 , no. 4 , pp. 796-804 . https://doi.org/10.1111/j.1365-2435.2010.01689.x |
op_relation |
https://research.rug.nl/en/publications/1f8b304b-c294-4e57-94d0-49421327449d |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1111/j.1365-2435.2010.01689.x |
container_title |
Functional Ecology |
container_volume |
24 |
container_issue |
4 |
container_start_page |
796 |
op_container_end_page |
804 |
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1802643079086211072 |