A simple route to single-nucleotide polymorphisms in a nonmodel species:identification and characterization of SNPs in the Artic ringed seal (Pusa hispida hispida)
Although single-nucleotide polymorphisms (SNPs) have become the marker of choice in the field of human genetics, these markers are only slowly emerging in ecological, evolutionary and conservation genetic analyses of nonmodel species. This is partly because of difficulties associated with the discov...
Published in: | Molecular Ecology Resources |
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2011
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Online Access: | https://hdl.handle.net/11370/11186b36-dcc5-415d-9539-7a247580c9ae https://research.rug.nl/en/publications/11186b36-dcc5-415d-9539-7a247580c9ae https://doi.org/10.1111/j.1755-0998.2010.02941.x |
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ftunigroningenpu:oai:pure.rug.nl:publications/11186b36-dcc5-415d-9539-7a247580c9ae 2024-06-02T08:02:18+00:00 A simple route to single-nucleotide polymorphisms in a nonmodel species:identification and characterization of SNPs in the Artic ringed seal (Pusa hispida hispida) Olsen, Morten Tange Volny, Veronica Hirsh Berube, Martine Dietz, Rune Lydersen, Christian Kovacs, Kit M. Dodd, Richard S. Palsboll, Per J. . 2011-03 https://hdl.handle.net/11370/11186b36-dcc5-415d-9539-7a247580c9ae https://research.rug.nl/en/publications/11186b36-dcc5-415d-9539-7a247580c9ae https://doi.org/10.1111/j.1755-0998.2010.02941.x eng eng https://research.rug.nl/en/publications/11186b36-dcc5-415d-9539-7a247580c9ae info:eu-repo/semantics/closedAccess Olsen , M T , Volny , V H , Berube , M , Dietz , R , Lydersen , C , Kovacs , K M , Dodd , R S & Palsboll , P J 2011 , ' A simple route to single-nucleotide polymorphisms in a nonmodel species : identification and characterization of SNPs in the Artic ringed seal (Pusa hispida hispida) ' , Molecular Ecology Resources , vol. 11 , pp. 9-19 . https://doi.org/10.1111/j.1755-0998.2010.02941.x Arctic discovery genomics natural populations single-nucleotide polymorphism ALLELE FREQUENCY-SPECTRUM HUMAN GENOME POPULATION PARAMETERS SEQUENCE VARIATION PHOCA-HISPIDA HAPLOTYPE RECONSTRUCTION DEMOGRAPHIC HISTORY REPRODUCTIVE RATES HUDSON-BAY MARKERS article 2011 ftunigroningenpu https://doi.org/10.1111/j.1755-0998.2010.02941.x 2024-05-07T19:28:23Z Although single-nucleotide polymorphisms (SNPs) have become the marker of choice in the field of human genetics, these markers are only slowly emerging in ecological, evolutionary and conservation genetic analyses of nonmodel species. This is partly because of difficulties associated with the discovery and characterization of SNP markers. Herein, we adopted a simple straightforward approach to identifying SNPs, based on screening of a random genomic library. In total, we identified 768 SNPs in the ringed seal, Pusa hispida hispida, in samples from Greenland and Svalbard. Using three seal samples, SNPs were discovered at a rate of one SNP per 402 bp, whereas re-sequencing of 96 seals increased the density to one SNP per 29 bp. Although applicable to any species of interest, the approach is especially well suited for SNP discovery in nonmodel organisms and is easily implemented in any standard genetics laboratory, circumventing the need for prior genomic data and use of next-generation sequencing facilities. Article in Journal/Newspaper Arctic Greenland Hudson Bay Phoca hispida Pusa hispida ringed seal Svalbard University of Groningen research database Arctic Greenland Hudson Hudson Bay Svalbard Molecular Ecology Resources 11 9 19 |
institution |
Open Polar |
collection |
University of Groningen research database |
op_collection_id |
ftunigroningenpu |
language |
English |
topic |
Arctic discovery genomics natural populations single-nucleotide polymorphism ALLELE FREQUENCY-SPECTRUM HUMAN GENOME POPULATION PARAMETERS SEQUENCE VARIATION PHOCA-HISPIDA HAPLOTYPE RECONSTRUCTION DEMOGRAPHIC HISTORY REPRODUCTIVE RATES HUDSON-BAY MARKERS |
spellingShingle |
Arctic discovery genomics natural populations single-nucleotide polymorphism ALLELE FREQUENCY-SPECTRUM HUMAN GENOME POPULATION PARAMETERS SEQUENCE VARIATION PHOCA-HISPIDA HAPLOTYPE RECONSTRUCTION DEMOGRAPHIC HISTORY REPRODUCTIVE RATES HUDSON-BAY MARKERS Olsen, Morten Tange Volny, Veronica Hirsh Berube, Martine Dietz, Rune Lydersen, Christian Kovacs, Kit M. Dodd, Richard S. Palsboll, Per J. . A simple route to single-nucleotide polymorphisms in a nonmodel species:identification and characterization of SNPs in the Artic ringed seal (Pusa hispida hispida) |
topic_facet |
Arctic discovery genomics natural populations single-nucleotide polymorphism ALLELE FREQUENCY-SPECTRUM HUMAN GENOME POPULATION PARAMETERS SEQUENCE VARIATION PHOCA-HISPIDA HAPLOTYPE RECONSTRUCTION DEMOGRAPHIC HISTORY REPRODUCTIVE RATES HUDSON-BAY MARKERS |
description |
Although single-nucleotide polymorphisms (SNPs) have become the marker of choice in the field of human genetics, these markers are only slowly emerging in ecological, evolutionary and conservation genetic analyses of nonmodel species. This is partly because of difficulties associated with the discovery and characterization of SNP markers. Herein, we adopted a simple straightforward approach to identifying SNPs, based on screening of a random genomic library. In total, we identified 768 SNPs in the ringed seal, Pusa hispida hispida, in samples from Greenland and Svalbard. Using three seal samples, SNPs were discovered at a rate of one SNP per 402 bp, whereas re-sequencing of 96 seals increased the density to one SNP per 29 bp. Although applicable to any species of interest, the approach is especially well suited for SNP discovery in nonmodel organisms and is easily implemented in any standard genetics laboratory, circumventing the need for prior genomic data and use of next-generation sequencing facilities. |
format |
Article in Journal/Newspaper |
author |
Olsen, Morten Tange Volny, Veronica Hirsh Berube, Martine Dietz, Rune Lydersen, Christian Kovacs, Kit M. Dodd, Richard S. Palsboll, Per J. . |
author_facet |
Olsen, Morten Tange Volny, Veronica Hirsh Berube, Martine Dietz, Rune Lydersen, Christian Kovacs, Kit M. Dodd, Richard S. Palsboll, Per J. . |
author_sort |
Olsen, Morten Tange |
title |
A simple route to single-nucleotide polymorphisms in a nonmodel species:identification and characterization of SNPs in the Artic ringed seal (Pusa hispida hispida) |
title_short |
A simple route to single-nucleotide polymorphisms in a nonmodel species:identification and characterization of SNPs in the Artic ringed seal (Pusa hispida hispida) |
title_full |
A simple route to single-nucleotide polymorphisms in a nonmodel species:identification and characterization of SNPs in the Artic ringed seal (Pusa hispida hispida) |
title_fullStr |
A simple route to single-nucleotide polymorphisms in a nonmodel species:identification and characterization of SNPs in the Artic ringed seal (Pusa hispida hispida) |
title_full_unstemmed |
A simple route to single-nucleotide polymorphisms in a nonmodel species:identification and characterization of SNPs in the Artic ringed seal (Pusa hispida hispida) |
title_sort |
simple route to single-nucleotide polymorphisms in a nonmodel species:identification and characterization of snps in the artic ringed seal (pusa hispida hispida) |
publishDate |
2011 |
url |
https://hdl.handle.net/11370/11186b36-dcc5-415d-9539-7a247580c9ae https://research.rug.nl/en/publications/11186b36-dcc5-415d-9539-7a247580c9ae https://doi.org/10.1111/j.1755-0998.2010.02941.x |
geographic |
Arctic Greenland Hudson Hudson Bay Svalbard |
geographic_facet |
Arctic Greenland Hudson Hudson Bay Svalbard |
genre |
Arctic Greenland Hudson Bay Phoca hispida Pusa hispida ringed seal Svalbard |
genre_facet |
Arctic Greenland Hudson Bay Phoca hispida Pusa hispida ringed seal Svalbard |
op_source |
Olsen , M T , Volny , V H , Berube , M , Dietz , R , Lydersen , C , Kovacs , K M , Dodd , R S & Palsboll , P J 2011 , ' A simple route to single-nucleotide polymorphisms in a nonmodel species : identification and characterization of SNPs in the Artic ringed seal (Pusa hispida hispida) ' , Molecular Ecology Resources , vol. 11 , pp. 9-19 . https://doi.org/10.1111/j.1755-0998.2010.02941.x |
op_relation |
https://research.rug.nl/en/publications/11186b36-dcc5-415d-9539-7a247580c9ae |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1111/j.1755-0998.2010.02941.x |
container_title |
Molecular Ecology Resources |
container_volume |
11 |
container_start_page |
9 |
op_container_end_page |
19 |
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1800746807086546944 |