Gut content metabarcoding of six crustaceans provides detailed diet description and insights into their roles as predators and scavengers
International audience While many studies have analyzed the diet of marine consumers using traditional morphology-based methods, the integral diet of many species of functionally important groups such as benthic invertebrates remains poorly resolved. This hinders our ability to precisely establish t...
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ftunigrenoble:oai:HAL:hal-04646798v1 2024-09-15T18:24:45+00:00 Gut content metabarcoding of six crustaceans provides detailed diet description and insights into their roles as predators and scavengers Boët, Pauline Kopp, Dorothée Méhault, Sonia Morfin, Marie Bonin, Aurélie Lejeune, Benjamin Dynamique et durabilité des écosystèmes : de la source à l’océan (DECOD) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut Agro Rennes Angers Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) Laboratoire d'Ecologie Alpine (LECA ) Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA) Centre d'Ecologie et des Sciences de la COnservation (CESCO) Muséum national d'Histoire naturelle (MNHN)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) 2024-08 https://hal.inrae.fr/hal-04646798 https://doi.org/10.1016/j.seares.2024.102509 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.seares.2024.102509 hal-04646798 https://hal.inrae.fr/hal-04646798 doi:10.1016/j.seares.2024.102509 WOS: 001250051900001 http://creativecommons.org/licenses/by/ ISSN: 1385-1101 EISSN: 1873-1414 Journal of Sea Research (JSR) https://hal.inrae.fr/hal-04646798 Journal of Sea Research (JSR), 2024, 200, pp.102509. ⟨10.1016/j.seares.2024.102509⟩ North-East Atlantic DNA molecular analysis Diet composition Benthic invertebrates Crustacean decapods Trophic ecology [SDV.SA.STP]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of fishery info:eu-repo/semantics/article Journal articles 2024 ftunigrenoble https://doi.org/10.1016/j.seares.2024.102509 2024-07-29T23:39:57Z International audience While many studies have analyzed the diet of marine consumers using traditional morphology-based methods, the integral diet of many species of functionally important groups such as benthic invertebrates remains poorly resolved. This hinders our ability to precisely establish trophic links among species, which is necessary to understand food webs and ecosystem dynamics. Here, we applied DNA metabarcoding to obtain a high resolution picture of the animal fraction of the diet of six species of crustaceans in the Bay of Biscay; the edible crab Cancer pagurus, the velvet crab Necora puber, the circular crab Atelecyclus undecimdentatus, the spider crab Maja brachydactyla, the shrimp Crangon crangon, and the hermit crab Pagurus bernhardus. We used two pairs of primers targeting the 18S rDNA (generalist) and 12S rDNA (fish-specific) regions to identify prey that makes up the animal fraction of the diet. Based on occurrence data, 54 prey taxa belonging to 5 different phylum mostly represented by teleost fishes and malacostracans were identified. Through a high-level of prey detection, results indicate a scavenging strategy across all consumers mostly based on the consumption of preys likely originating from fishing discards. This study also reveals patterns of niche overlaps among consumers differentiating C. crangon from others and suggesting high overlap in animal resource use between N. puber and M. brachydactyla as well as C. pagurus. High resolution diet description revealed the complexity of trophic interactions on a local scale. Article in Journal/Newspaper North East Atlantic Université Grenoble Alpes: HAL Journal of Sea Research 200 102509 |
institution |
Open Polar |
collection |
Université Grenoble Alpes: HAL |
op_collection_id |
ftunigrenoble |
language |
English |
topic |
North-East Atlantic DNA molecular analysis Diet composition Benthic invertebrates Crustacean decapods Trophic ecology [SDV.SA.STP]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of fishery |
spellingShingle |
North-East Atlantic DNA molecular analysis Diet composition Benthic invertebrates Crustacean decapods Trophic ecology [SDV.SA.STP]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of fishery Boët, Pauline Kopp, Dorothée Méhault, Sonia Morfin, Marie Bonin, Aurélie Lejeune, Benjamin Gut content metabarcoding of six crustaceans provides detailed diet description and insights into their roles as predators and scavengers |
topic_facet |
North-East Atlantic DNA molecular analysis Diet composition Benthic invertebrates Crustacean decapods Trophic ecology [SDV.SA.STP]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of fishery |
description |
International audience While many studies have analyzed the diet of marine consumers using traditional morphology-based methods, the integral diet of many species of functionally important groups such as benthic invertebrates remains poorly resolved. This hinders our ability to precisely establish trophic links among species, which is necessary to understand food webs and ecosystem dynamics. Here, we applied DNA metabarcoding to obtain a high resolution picture of the animal fraction of the diet of six species of crustaceans in the Bay of Biscay; the edible crab Cancer pagurus, the velvet crab Necora puber, the circular crab Atelecyclus undecimdentatus, the spider crab Maja brachydactyla, the shrimp Crangon crangon, and the hermit crab Pagurus bernhardus. We used two pairs of primers targeting the 18S rDNA (generalist) and 12S rDNA (fish-specific) regions to identify prey that makes up the animal fraction of the diet. Based on occurrence data, 54 prey taxa belonging to 5 different phylum mostly represented by teleost fishes and malacostracans were identified. Through a high-level of prey detection, results indicate a scavenging strategy across all consumers mostly based on the consumption of preys likely originating from fishing discards. This study also reveals patterns of niche overlaps among consumers differentiating C. crangon from others and suggesting high overlap in animal resource use between N. puber and M. brachydactyla as well as C. pagurus. High resolution diet description revealed the complexity of trophic interactions on a local scale. |
author2 |
Dynamique et durabilité des écosystèmes : de la source à l’océan (DECOD) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut Agro Rennes Angers Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) Laboratoire d'Ecologie Alpine (LECA ) Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA) Centre d'Ecologie et des Sciences de la COnservation (CESCO) Muséum national d'Histoire naturelle (MNHN)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Boët, Pauline Kopp, Dorothée Méhault, Sonia Morfin, Marie Bonin, Aurélie Lejeune, Benjamin |
author_facet |
Boët, Pauline Kopp, Dorothée Méhault, Sonia Morfin, Marie Bonin, Aurélie Lejeune, Benjamin |
author_sort |
Boët, Pauline |
title |
Gut content metabarcoding of six crustaceans provides detailed diet description and insights into their roles as predators and scavengers |
title_short |
Gut content metabarcoding of six crustaceans provides detailed diet description and insights into their roles as predators and scavengers |
title_full |
Gut content metabarcoding of six crustaceans provides detailed diet description and insights into their roles as predators and scavengers |
title_fullStr |
Gut content metabarcoding of six crustaceans provides detailed diet description and insights into their roles as predators and scavengers |
title_full_unstemmed |
Gut content metabarcoding of six crustaceans provides detailed diet description and insights into their roles as predators and scavengers |
title_sort |
gut content metabarcoding of six crustaceans provides detailed diet description and insights into their roles as predators and scavengers |
publisher |
HAL CCSD |
publishDate |
2024 |
url |
https://hal.inrae.fr/hal-04646798 https://doi.org/10.1016/j.seares.2024.102509 |
genre |
North East Atlantic |
genre_facet |
North East Atlantic |
op_source |
ISSN: 1385-1101 EISSN: 1873-1414 Journal of Sea Research (JSR) https://hal.inrae.fr/hal-04646798 Journal of Sea Research (JSR), 2024, 200, pp.102509. ⟨10.1016/j.seares.2024.102509⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.seares.2024.102509 hal-04646798 https://hal.inrae.fr/hal-04646798 doi:10.1016/j.seares.2024.102509 WOS: 001250051900001 |
op_rights |
http://creativecommons.org/licenses/by/ |
op_doi |
https://doi.org/10.1016/j.seares.2024.102509 |
container_title |
Journal of Sea Research |
container_volume |
200 |
container_start_page |
102509 |
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1810465177736314880 |