The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia
International audience A mercury-resistant bacterial strain has been isolated from a rock of the Idrija mercury mine in Slovenia. The rock had 19 g carbon and 2952 mg mercury (Hg) per kg. Mass spectrometry and DNA sequencing showed that the bacterium belongs to the Pseudomonas genus. It is called Ps...
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ftunigrenoble:oai:HAL:hal-02469580v1 2024-04-28T08:36:05+00:00 The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia Bourdineaud, Jean-Paul Durn, Goran Režun, Bojan Manceau, Alain Hrenović, Jasna Microbiologie Fondamentale et Pathogénicité (MFP) Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS) University of Zagreb Institut des Sciences de la Terre (ISTerre) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Université Gustave Eiffel-Université Grenoble Alpes (UGA) Croatian Science Foundation (project no. IP-2014-09-5656).The French National Research Agency (ANR). 2020 https://hal.science/hal-02469580 https://hal.science/hal-02469580/document https://hal.science/hal-02469580/file/Article%20Chemosphere%202020-version%20Hal.pdf https://doi.org/10.1016/j.chemosphere.2020.126002 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemosphere.2020.126002 hal-02469580 https://hal.science/hal-02469580 https://hal.science/hal-02469580/document https://hal.science/hal-02469580/file/Article%20Chemosphere%202020-version%20Hal.pdf doi:10.1016/j.chemosphere.2020.126002 info:eu-repo/semantics/OpenAccess ISSN: 0045-6535 EISSN: 1879-1298 Chemosphere https://hal.science/hal-02469580 Chemosphere, 2020, 248, pp.126002. ⟨10.1016/j.chemosphere.2020.126002⟩ Mercury mer operon Pseudomonas idrijaensis Tn5041 Idrija mine XANES spectroscopy [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2020 ftunigrenoble https://doi.org/10.1016/j.chemosphere.2020.126002 2024-04-18T03:37:33Z International audience A mercury-resistant bacterial strain has been isolated from a rock of the Idrija mercury mine in Slovenia. The rock had 19 g carbon and 2952 mg mercury (Hg) per kg. Mass spectrometry and DNA sequencing showed that the bacterium belongs to the Pseudomonas genus. It is called Pseudomonas idrijaensis. This bacterial strain is sensitive to methylmercury (MeHg) like the reference P. aeruginosa strain PAO1, and is resistant to divalent mercury (Hg(II)) in contrast to PAO1. This difference could be attributed to the presence of the mer operon yet deprived of the merB gene encoding the organomercurial lyase, on the basis of whole genome sequencing. The P. idrijaensis mer operon displays the RTPCADE organization and is contained in the Tn5041 transposon. This transposon identified here occurs in other Gram-negative Hg-resistant strains isolated from mercury ores, aquatic systems and soils, including Pseudomonas strains from 15,000 to 40,000 years old Siberian permafrost. When P. idrijaensis was exposed to mercury chloride, two intracellular Hg species were identified by high energy-resolution XANES spectroscopy, a dithiolate Hg(SR)2 and a tetrathiolate Hg(SR)4 complex. P. idrijaensis had a much higher [Hg(SR)2]/[Hg(SR)4] molar ratio than bacteria lacking the mer operon when exposed to 4 µg Hg2+/L - resulting in an intracellular accumulation of 4.3 µg Hg/g dw. A higher amount of the Hg(SR)2 complex provides a chemical signature for the expression of the dicysteinate Mer proteins in response to mercury toxicity. Article in Journal/Newspaper permafrost Université Grenoble Alpes: HAL Chemosphere 248 126002 |
institution |
Open Polar |
collection |
Université Grenoble Alpes: HAL |
op_collection_id |
ftunigrenoble |
language |
English |
topic |
Mercury mer operon Pseudomonas idrijaensis Tn5041 Idrija mine XANES spectroscopy [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDE]Environmental Sciences |
spellingShingle |
Mercury mer operon Pseudomonas idrijaensis Tn5041 Idrija mine XANES spectroscopy [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDE]Environmental Sciences Bourdineaud, Jean-Paul Durn, Goran Režun, Bojan Manceau, Alain Hrenović, Jasna The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia |
topic_facet |
Mercury mer operon Pseudomonas idrijaensis Tn5041 Idrija mine XANES spectroscopy [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDE]Environmental Sciences |
description |
International audience A mercury-resistant bacterial strain has been isolated from a rock of the Idrija mercury mine in Slovenia. The rock had 19 g carbon and 2952 mg mercury (Hg) per kg. Mass spectrometry and DNA sequencing showed that the bacterium belongs to the Pseudomonas genus. It is called Pseudomonas idrijaensis. This bacterial strain is sensitive to methylmercury (MeHg) like the reference P. aeruginosa strain PAO1, and is resistant to divalent mercury (Hg(II)) in contrast to PAO1. This difference could be attributed to the presence of the mer operon yet deprived of the merB gene encoding the organomercurial lyase, on the basis of whole genome sequencing. The P. idrijaensis mer operon displays the RTPCADE organization and is contained in the Tn5041 transposon. This transposon identified here occurs in other Gram-negative Hg-resistant strains isolated from mercury ores, aquatic systems and soils, including Pseudomonas strains from 15,000 to 40,000 years old Siberian permafrost. When P. idrijaensis was exposed to mercury chloride, two intracellular Hg species were identified by high energy-resolution XANES spectroscopy, a dithiolate Hg(SR)2 and a tetrathiolate Hg(SR)4 complex. P. idrijaensis had a much higher [Hg(SR)2]/[Hg(SR)4] molar ratio than bacteria lacking the mer operon when exposed to 4 µg Hg2+/L - resulting in an intracellular accumulation of 4.3 µg Hg/g dw. A higher amount of the Hg(SR)2 complex provides a chemical signature for the expression of the dicysteinate Mer proteins in response to mercury toxicity. |
author2 |
Microbiologie Fondamentale et Pathogénicité (MFP) Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS) University of Zagreb Institut des Sciences de la Terre (ISTerre) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)-Université Gustave Eiffel-Université Grenoble Alpes (UGA) Croatian Science Foundation (project no. IP-2014-09-5656).The French National Research Agency (ANR). |
format |
Article in Journal/Newspaper |
author |
Bourdineaud, Jean-Paul Durn, Goran Režun, Bojan Manceau, Alain Hrenović, Jasna |
author_facet |
Bourdineaud, Jean-Paul Durn, Goran Režun, Bojan Manceau, Alain Hrenović, Jasna |
author_sort |
Bourdineaud, Jean-Paul |
title |
The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia |
title_short |
The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia |
title_full |
The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia |
title_fullStr |
The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia |
title_full_unstemmed |
The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia |
title_sort |
chemical species of mercury accumulated by pseudomonas idrijaensis, a bacterium from a rock of the idrija mercury mine, slovenia |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.science/hal-02469580 https://hal.science/hal-02469580/document https://hal.science/hal-02469580/file/Article%20Chemosphere%202020-version%20Hal.pdf https://doi.org/10.1016/j.chemosphere.2020.126002 |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
ISSN: 0045-6535 EISSN: 1879-1298 Chemosphere https://hal.science/hal-02469580 Chemosphere, 2020, 248, pp.126002. ⟨10.1016/j.chemosphere.2020.126002⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemosphere.2020.126002 hal-02469580 https://hal.science/hal-02469580 https://hal.science/hal-02469580/document https://hal.science/hal-02469580/file/Article%20Chemosphere%202020-version%20Hal.pdf doi:10.1016/j.chemosphere.2020.126002 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.chemosphere.2020.126002 |
container_title |
Chemosphere |
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248 |
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126002 |
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