Synthesis of lipase/silica biocatalysts through the immobilization of CALB on porous SBA-15 and their application on the resolution of pharmaceutical derivatives and on nutraceutical enrichment of natural oil
A recombinant lipase from Candida antarctica, LIPB, was obtained through fermentation using an alternative low-cost medium and immobilized on the distinct mesoporous silica particles. The performance and versatility of the new biocatalysts was evaluated, for the first time, on two biotechnological i...
Published in: | Molecular Catalysis |
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Language: | English |
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ftunifrgnorteir:oai:https://repositorio.ufrn.br:123456789/45062 2023-05-15T13:45:41+02:00 Synthesis of lipase/silica biocatalysts through the immobilization of CALB on porous SBA-15 and their application on the resolution of pharmaceutical derivatives and on nutraceutical enrichment of natural oil Cipolattia, Eliane Pereira Rios, Nathalia Saraiva Sousa, Joab Sampaio Robert, Julia de Macedo Silva, Angelo Amaro Theodoro Pinto, Martina C. C. Simas, Alessandro Bolis Costa García, Enrique Vilarrasa Lafuente, Roberto Fernandez Gonçalves, Luciana Rocha Barros Freire, Denise Maria Guimarães Manoel, Evelin Andrade 2021-04 https://repositorio.ufrn.br/handle/123456789/45062 https://doi.org/10.1016/j.mcat.2021.111529 en eng Elsevier CIPOLATTI, Eliane Pereira; RIOS, Nathalia Saraiva; SOUSA, Joab Sampaio; ROBERT, Julia de Macedo; SILVA, Angelo Amaro Theodoro da; PINTO, Martina C.C.; SIMAS, Alessandro Bolis Costa; VILARRASA-GARCÍA, Enrique; FERNANDEZ-LAFUENTE, Roberto; GONÇALVES, Luciana Rocha Barros. Synthesis of lipase/silica biocatalysts through the immobilization of CALB on porous SBA-15 and their application on the resolution of pharmaceutical derivatives and on nutraceutical enrichment of natural oil. Molecular Catalysis, [S.L.], v. 505, p. 111529-1, abr. 2021. Disponível em: https://www.sciencedirect.com/science/article/pii/S2468823121001462?casa_token=_J4dy4HY-8YAAAAA:CzTa73_HJppGDajuJK-a59aGAd7Ft8qe9SBRSV_sFL-YxqnDZq8pjYrQ4ivWKCCHbTL5cuj5-qs7#!. Acesso em: 29 nov. 2021. https://doi.org/10.1016/j.mcat.2021.111529. 2468-8231 https://repositorio.ufrn.br/handle/123456789/45062 doi:10.1016/j.mcat.2021.111529 Myo-inositol Coconut oil Lipase immobilization Mesoporous silica Funcionalized particles article 2021 ftunifrgnorteir https://doi.org/10.1016/j.mcat.2021.111529 2023-02-19T00:36:36Z A recombinant lipase from Candida antarctica, LIPB, was obtained through fermentation using an alternative low-cost medium and immobilized on the distinct mesoporous silica particles. The performance and versatility of the new biocatalysts was evaluated, for the first time, on two biotechnological interest reactions: resolution of racemic myo-inositol derivative and acidolysis reaction of coconut oil. For the racemate, the covalent biocatalyst SBA-15-F-APTES-GA-LIPB exhibited the highest conversions and enantiomeric excess of up to 99 %. It was the first time that covalent biocatalyst was used for resolution of racemic myo-inositol derivative. The most active biocatalyst (SBA-15-F-APTES-GA-LIPB) was used on ten different reaction cycles maintaining its activity, highlighting the operational stability of the developed biocatalyst. Moreover, it was possible to produce food oils enriched with polyunsaturated fatty acids (PUFA), obtaining lipids containing 181.6 mg of EPA and DHA in coconut oil. The preliminary economic analyses also indicate that the enzymatic production of such nutraceuticals and pharmaceutics compounds may be economically feasible, principally exploring low-cost strategies for enzyme production Article in Journal/Newspaper Antarc* Antarctica Universidade Federal do Rio Grande do Norte: Repositório Institucional (RI UFRN) Molecular Catalysis 505 111529 |
institution |
Open Polar |
collection |
Universidade Federal do Rio Grande do Norte: Repositório Institucional (RI UFRN) |
op_collection_id |
ftunifrgnorteir |
language |
English |
topic |
Myo-inositol Coconut oil Lipase immobilization Mesoporous silica Funcionalized particles |
spellingShingle |
Myo-inositol Coconut oil Lipase immobilization Mesoporous silica Funcionalized particles Cipolattia, Eliane Pereira Rios, Nathalia Saraiva Sousa, Joab Sampaio Robert, Julia de Macedo Silva, Angelo Amaro Theodoro Pinto, Martina C. C. Simas, Alessandro Bolis Costa García, Enrique Vilarrasa Lafuente, Roberto Fernandez Gonçalves, Luciana Rocha Barros Freire, Denise Maria Guimarães Manoel, Evelin Andrade Synthesis of lipase/silica biocatalysts through the immobilization of CALB on porous SBA-15 and their application on the resolution of pharmaceutical derivatives and on nutraceutical enrichment of natural oil |
topic_facet |
Myo-inositol Coconut oil Lipase immobilization Mesoporous silica Funcionalized particles |
description |
A recombinant lipase from Candida antarctica, LIPB, was obtained through fermentation using an alternative low-cost medium and immobilized on the distinct mesoporous silica particles. The performance and versatility of the new biocatalysts was evaluated, for the first time, on two biotechnological interest reactions: resolution of racemic myo-inositol derivative and acidolysis reaction of coconut oil. For the racemate, the covalent biocatalyst SBA-15-F-APTES-GA-LIPB exhibited the highest conversions and enantiomeric excess of up to 99 %. It was the first time that covalent biocatalyst was used for resolution of racemic myo-inositol derivative. The most active biocatalyst (SBA-15-F-APTES-GA-LIPB) was used on ten different reaction cycles maintaining its activity, highlighting the operational stability of the developed biocatalyst. Moreover, it was possible to produce food oils enriched with polyunsaturated fatty acids (PUFA), obtaining lipids containing 181.6 mg of EPA and DHA in coconut oil. The preliminary economic analyses also indicate that the enzymatic production of such nutraceuticals and pharmaceutics compounds may be economically feasible, principally exploring low-cost strategies for enzyme production |
format |
Article in Journal/Newspaper |
author |
Cipolattia, Eliane Pereira Rios, Nathalia Saraiva Sousa, Joab Sampaio Robert, Julia de Macedo Silva, Angelo Amaro Theodoro Pinto, Martina C. C. Simas, Alessandro Bolis Costa García, Enrique Vilarrasa Lafuente, Roberto Fernandez Gonçalves, Luciana Rocha Barros Freire, Denise Maria Guimarães Manoel, Evelin Andrade |
author_facet |
Cipolattia, Eliane Pereira Rios, Nathalia Saraiva Sousa, Joab Sampaio Robert, Julia de Macedo Silva, Angelo Amaro Theodoro Pinto, Martina C. C. Simas, Alessandro Bolis Costa García, Enrique Vilarrasa Lafuente, Roberto Fernandez Gonçalves, Luciana Rocha Barros Freire, Denise Maria Guimarães Manoel, Evelin Andrade |
author_sort |
Cipolattia, Eliane Pereira |
title |
Synthesis of lipase/silica biocatalysts through the immobilization of CALB on porous SBA-15 and their application on the resolution of pharmaceutical derivatives and on nutraceutical enrichment of natural oil |
title_short |
Synthesis of lipase/silica biocatalysts through the immobilization of CALB on porous SBA-15 and their application on the resolution of pharmaceutical derivatives and on nutraceutical enrichment of natural oil |
title_full |
Synthesis of lipase/silica biocatalysts through the immobilization of CALB on porous SBA-15 and their application on the resolution of pharmaceutical derivatives and on nutraceutical enrichment of natural oil |
title_fullStr |
Synthesis of lipase/silica biocatalysts through the immobilization of CALB on porous SBA-15 and their application on the resolution of pharmaceutical derivatives and on nutraceutical enrichment of natural oil |
title_full_unstemmed |
Synthesis of lipase/silica biocatalysts through the immobilization of CALB on porous SBA-15 and their application on the resolution of pharmaceutical derivatives and on nutraceutical enrichment of natural oil |
title_sort |
synthesis of lipase/silica biocatalysts through the immobilization of calb on porous sba-15 and their application on the resolution of pharmaceutical derivatives and on nutraceutical enrichment of natural oil |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://repositorio.ufrn.br/handle/123456789/45062 https://doi.org/10.1016/j.mcat.2021.111529 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
CIPOLATTI, Eliane Pereira; RIOS, Nathalia Saraiva; SOUSA, Joab Sampaio; ROBERT, Julia de Macedo; SILVA, Angelo Amaro Theodoro da; PINTO, Martina C.C.; SIMAS, Alessandro Bolis Costa; VILARRASA-GARCÍA, Enrique; FERNANDEZ-LAFUENTE, Roberto; GONÇALVES, Luciana Rocha Barros. Synthesis of lipase/silica biocatalysts through the immobilization of CALB on porous SBA-15 and their application on the resolution of pharmaceutical derivatives and on nutraceutical enrichment of natural oil. Molecular Catalysis, [S.L.], v. 505, p. 111529-1, abr. 2021. Disponível em: https://www.sciencedirect.com/science/article/pii/S2468823121001462?casa_token=_J4dy4HY-8YAAAAA:CzTa73_HJppGDajuJK-a59aGAd7Ft8qe9SBRSV_sFL-YxqnDZq8pjYrQ4ivWKCCHbTL5cuj5-qs7#!. Acesso em: 29 nov. 2021. https://doi.org/10.1016/j.mcat.2021.111529. 2468-8231 https://repositorio.ufrn.br/handle/123456789/45062 doi:10.1016/j.mcat.2021.111529 |
op_doi |
https://doi.org/10.1016/j.mcat.2021.111529 |
container_title |
Molecular Catalysis |
container_volume |
505 |
container_start_page |
111529 |
_version_ |
1766229813310259200 |