Leishmania in skin of Rattus rattus from the urban area in the Corrientes city, Argentina
Objective: the objective of the present work was the detection of Leishmania sp. in Rattus rattus tail base skin through polymerase chain reaction (PCR) techniques. Material and Method: We analyzed 45 Rattus rattus skin samples from the urban area of the city of Corrientes in Argentina. The Leishman...
| Published in: | Revista MVZ Córdoba |
|---|---|
| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | Spanish English |
| Published: |
Universidad de Córdoba
2022
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| Subjects: | |
| Online Access: | https://revistamvz.unicordoba.edu.co/article/view/2546 |
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Universidad de Córdoba Portal de Revistas |
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| language |
Spanish English |
| topic |
Leishmania DNA epidemiological chain leishmaniasis prevalence black rat polymerase chain reaction ADN de Leishmania Cadena epidemiológica prevalencia rata negra reacción en cadena de la polimerasa |
| spellingShingle |
Leishmania DNA epidemiological chain leishmaniasis prevalence black rat polymerase chain reaction ADN de Leishmania Cadena epidemiológica prevalencia rata negra reacción en cadena de la polimerasa Ramirez, Gabriela Verónica Ruiz, Raquel Mónica Alegre, Elsa Agustina Leishmania in skin of Rattus rattus from the urban area in the Corrientes city, Argentina |
| topic_facet |
Leishmania DNA epidemiological chain leishmaniasis prevalence black rat polymerase chain reaction ADN de Leishmania Cadena epidemiológica prevalencia rata negra reacción en cadena de la polimerasa |
| description |
Objective: the objective of the present work was the detection of Leishmania sp. in Rattus rattus tail base skin through polymerase chain reaction (PCR) techniques. Material and Method: We analyzed 45 Rattus rattus skin samples from the urban area of the city of Corrientes in Argentina. The Leishmania spp detection was performed by nested PCR technique. Results: Leishmania DNA was detected in 22 samples out of 45 processed (49%) in the first round of amplification, and in 14 samples (31%) in the second round (31%). Conclusions: These results contribute to increase the existing information in our region on the possible relationship between Leishmania and Rattus rattus, considering the high prevalence found in skin added to the total absence of lesions. Other aspects should be further studied to establish the role of these animals in the epidemiological chain of the disease in an urban area endemic to leishmaniasis in other animal species. Objetivo: detectar Leishmania sp. en piel de cola de Rattus rattus a través de técnicas de reacción en cadena de la polimerasa. Material y Método: Se analizaron 45 muestras de piel de Rattus rattus del área urbana de la ciudad de Corrientes, Argentina. La detección de Leishmania sp se realizó mediante técnicas de PCR anidada. Resultados: En la primera ronda de amplificación se detectó ADN de Leishmania en 22 muestras de 45 procesadas (49%) y en 14 muestras en la segunda ronda (31%). Conclusiones: Estos resultados contribuyen a aumentar la información existente en nuestra región sobre la posible relación entre Leishmania y Rattus rattus, teniendo en cuenta la alta prevalencia encontrada en piel sumado a la total ausencia de lesiones. Otros aspectos deberán seguir estudiándose para establecer el rol de estos animales en la cadena epidemiológica de la enfermedad en una zona urbana endémica a leishmaniasis en otras especies animales. |
| format |
Article in Journal/Newspaper |
| author |
Ramirez, Gabriela Verónica Ruiz, Raquel Mónica Alegre, Elsa Agustina |
| author_facet |
Ramirez, Gabriela Verónica Ruiz, Raquel Mónica Alegre, Elsa Agustina |
| author_sort |
Ramirez, Gabriela Verónica |
| title |
Leishmania in skin of Rattus rattus from the urban area in the Corrientes city, Argentina |
| title_short |
Leishmania in skin of Rattus rattus from the urban area in the Corrientes city, Argentina |
| title_full |
Leishmania in skin of Rattus rattus from the urban area in the Corrientes city, Argentina |
| title_fullStr |
Leishmania in skin of Rattus rattus from the urban area in the Corrientes city, Argentina |
| title_full_unstemmed |
Leishmania in skin of Rattus rattus from the urban area in the Corrientes city, Argentina |
| title_sort |
leishmania in skin of rattus rattus from the urban area in the corrientes city, argentina |
| publisher |
Universidad de Córdoba |
| publishDate |
2022 |
| url |
https://revistamvz.unicordoba.edu.co/article/view/2546 |
| long_lat |
ENVELOPE(-67.600,-67.600,-67.450,-67.450) ENVELOPE(19.216,19.216,69.928,69.928) |
| geographic |
Alta Argentina Cadena Rata |
| geographic_facet |
Alta Argentina Cadena Rata |
| genre |
Rattus rattus |
| genre_facet |
Rattus rattus |
| op_source |
Journal MVZ Cordoba; Vol. 27 No. 3 (2022): Vol. 27 Núm. 3 (2022): Revista MVZ Córdoba Volumen 27(3) Septiembre-Diciembre 2022; e2546 Revista MVZ Córdoba; Vol. 27 Núm. 3 (2022): Vol. 27 Núm. 3 (2022): Revista MVZ Córdoba Volumen 27(3) Septiembre-Diciembre 2022; e2546 1909-0544 0122-0268 10.21897/rmvz.v27.n3.2022 |
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https://revistamvz.unicordoba.edu.co/article/view/2546/4860 https://revistamvz.unicordoba.edu.co/article/view/2546/4861 https://revistamvz.unicordoba.edu.co/article/view/2546/4865 https://revistamvz.unicordoba.edu.co/article/view/2546/4862 https://revistamvz.unicordoba.edu.co/article/view/2546/4863 https://revistamvz.unicordoba.edu.co/article/view/2546/4864 1. Germano M, Salomón M, Neira G, Lozano E, Mackern Oberti J, Cargnelutti D. Leishmaniasis in the Argentine Republic: Temporal and geographical distribution from 2013 to 2017. Asian Pac J Trop Med. 2019; 12(7):300-305. http://dx.doi.org/10.4103/1995-7645.262073 2. Roque A, Jansen A. Wild and synanthropic reservoirs of Leishmania species in the Americas. Int J Parasitol Parasites Wildl. 2014; 3(3):251-262. http://dx.doi.org/10.1016/j.ijppaw.2014.08.004 3. Caldart E, Freire R, Ferreira F, Ruffolo B, Sbeghen M, Mareze M, et al. Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis. Rev Bras Parasitol Vet. 2017; 26(1):17-27. https://doi.org/10.1590/S1984-29612017001 4. McFarlane R, Sleigh A, McMichael T. Synanthropy of wild mammals as a determinant of emerging infectious diseases in the Asian–Australasian region. EcoHealth. 2012; 9:24-35. https://doi.org/10.1007/s10393-012-0763-9 5. Faust C, Dobson A, Gottdenker N, Bloomfield L, McCallum H, Gillespie T, et al. Null expectations for disease dynamics in shrinking habitat: dilution or amplification? Phil Trans R Soc B. 2017; 372(1722):20160173. https://doi.org/10.1098/rstb.2016.0173 6. Ruiz R, Bastiani C, De Biasio M, Alegre E, Ramírez N. Detección de Leishmania sp. en Rattus rattus de la ciudad de Corrientes, Argentina. Arch Med Vet. 2015; 47(3):401-407. https://dx.doi.org/10.4067/S0301-732X2015000300020 7. Fernández M, Fraschina J, Acardi S, Liotta D, Lestani E, Giuliani M, et al. Assessment of the role of small mammals in the transmission cycle of tegumentary leishmaniasis and first report of natural infection with Leishmania braziliensis in two sigmodontines in northeastern Argentina. Parasitology Research. 2018; 117(2):405-412. https://doi.org/10.1007/s00436-017-5714-5 8. Corps B. The effect of graft thickness, donor site and graft-bed on graft shrinkaie in the hooded rat. Br J Plast Surg. 1969; 22:125-133. https://doi.org/10.1016/S0007-1226(69)80053-6 9. Alegre E, De Biasio M, Ramirez N, Ruiz R, Bastiani C. Detección y diferenciación molecular de Leptospiras sp. utilizando diferentes técnicas de extracción de ADN. Rev Vet. 2013; 24(1):53-55. http://dx.doi.org/10.30972/vet.2411151 10. Marcelino A, Ferreira E, Avendanha J, Costa C, Chiarelli D, Almeida G, et al. Molecular detection of Leishmania braziliensis in Rattus norvegicus in an area endemic for cutaneous leishmaniasis in Brazil. Veterinary Parasitology. 2011; 183(1-2):54-58. https://doi.org/10.1016/j.vetpar.2011.06.019 11. Akhavan A, Mirhendi H, Khamesipour A, Alimohammadian M, Rassi Y, Bates P, et al. Leishmania species: detection and identification by nested PCR assay from skin samples of rodent reservoirs. Exp Parasitol. 2010; 126:552–556. https://doi.org/10.1016/j.exppara.2010.06.003 12. de Castro Ferreira E, Cruz I, Cañavate C, de Melo L, Pereira A, Madeira F, et al. Mixed infection of Leishmania infantum and Leishmania braziliensis in rodents from endemic urban area of the New World. BMC Vet Res. 2015; 11(1): 1-7. https://doi.org/10.1186/s12917-015-0392-y 13. Quaresma P, Rêgo F, Botelho H, Silva S, Moura A Jr, Teixeira R. Wild, synanthropic and domestic hosts of Leishmania in an endemic area of cutaneous leishmaniasis in Minas Gerais State, Brazil. Trans R Soc Trop Med Hyg. 2011; 105(10):579-585. http://dx.doi.org/10.1016/j.trstmh.2011.07.005 14. Parhizkari M, Motazedian M, Asgari Q, Mehrabani D. The PCR-based detection of Leishmania major in Mus musculus and other rodents caught in Southern Iran: A guide to sample selection. Ann Trop Med Parasitol. 2011; 105:319–323. https://doi.org/10.1179/136485911X12987676649827 15. Pereira A, Ferreira E, Lima A, Tonelli G, Rêgo F, Paglia A, et al. Detection of Leishmania spp in silvatic mammals and isolation of Leishmania (Viannia) braziliensis from Rattus rattus in an endemic area for leishmaniasis in Minas Gerais State, Brazil. PLoS One. 2017; 12(11):e0187704. https://doi.org/10.1371/journal.pone.0187704 16. Lima B, Dantas-Torres F, Carvalho M, Marinho-Junior J, Almeida E, Brito M. Small mammals as hosts of Leishmania spp. in a highly endemic area for zoonotic leishmaniasis in north-eastern Brazil. Trans R Soc Trop Med Hyg. 2013; 107(9):592-597. http://dx.doi.org/10.1093/trstmh/trt062 17. de Oliveira Lara-Silva F, Andrade Barata R, Monteiro Michalsky E, de Castro Ferreira E, Garcia Lopes M, da Costa Pinheiro A, et al. Rattus norvegicus (Rodentia: Muridae) Infected by Leishmania (Leishmania) infantum (syn. Le. chagasi) in Brazil. Biomed Res Int. 2014; 2014: 1-7. https://doi.org/10.1155/2014/592986 18. Andrade M, Courtenay O, Brito M, Carvalho F, Carvalho A, Soares F, et al. Infectiousness of Sylvatic and Synanthropic Small Rodents Implicates a Multi-host Reservoir of Leishmania (Viannia) braziliensis. PLoS Negl Trop Dis. 2015. 9(10):e0004137. https://doi.org/10.1371/journal.pntd.0004137 https://revistamvz.unicordoba.edu.co/article/view/2546 |
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Derechos de autor 2022 Gabriela Verónica Ramirez, Raquel Mónica Ruiz, Elsa Agustina Alegre https://creativecommons.org/licenses/by-nc-sa/4.0 |
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ftunicordobacojs:oai:ojs.revistas.unicordoba.edu.co:article/2546 2023-05-15T18:04:37+02:00 Leishmania in skin of Rattus rattus from the urban area in the Corrientes city, Argentina Leishmania en piel de Rattus rattus de zona urbana de la Ciudad de Corrientes, Argentina Ramirez, Gabriela Verónica Ruiz, Raquel Mónica Alegre, Elsa Agustina 2022-09-01 application/pdf audio/mpeg https://revistamvz.unicordoba.edu.co/article/view/2546 spa eng spa eng Universidad de Córdoba https://revistamvz.unicordoba.edu.co/article/view/2546/4860 https://revistamvz.unicordoba.edu.co/article/view/2546/4861 https://revistamvz.unicordoba.edu.co/article/view/2546/4865 https://revistamvz.unicordoba.edu.co/article/view/2546/4862 https://revistamvz.unicordoba.edu.co/article/view/2546/4863 https://revistamvz.unicordoba.edu.co/article/view/2546/4864 1. Germano M, Salomón M, Neira G, Lozano E, Mackern Oberti J, Cargnelutti D. Leishmaniasis in the Argentine Republic: Temporal and geographical distribution from 2013 to 2017. Asian Pac J Trop Med. 2019; 12(7):300-305. http://dx.doi.org/10.4103/1995-7645.262073 2. Roque A, Jansen A. Wild and synanthropic reservoirs of Leishmania species in the Americas. Int J Parasitol Parasites Wildl. 2014; 3(3):251-262. http://dx.doi.org/10.1016/j.ijppaw.2014.08.004 3. Caldart E, Freire R, Ferreira F, Ruffolo B, Sbeghen M, Mareze M, et al. Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis. Rev Bras Parasitol Vet. 2017; 26(1):17-27. https://doi.org/10.1590/S1984-29612017001 4. McFarlane R, Sleigh A, McMichael T. Synanthropy of wild mammals as a determinant of emerging infectious diseases in the Asian–Australasian region. EcoHealth. 2012; 9:24-35. https://doi.org/10.1007/s10393-012-0763-9 5. Faust C, Dobson A, Gottdenker N, Bloomfield L, McCallum H, Gillespie T, et al. Null expectations for disease dynamics in shrinking habitat: dilution or amplification? Phil Trans R Soc B. 2017; 372(1722):20160173. https://doi.org/10.1098/rstb.2016.0173 6. Ruiz R, Bastiani C, De Biasio M, Alegre E, Ramírez N. Detección de Leishmania sp. en Rattus rattus de la ciudad de Corrientes, Argentina. Arch Med Vet. 2015; 47(3):401-407. https://dx.doi.org/10.4067/S0301-732X2015000300020 7. Fernández M, Fraschina J, Acardi S, Liotta D, Lestani E, Giuliani M, et al. Assessment of the role of small mammals in the transmission cycle of tegumentary leishmaniasis and first report of natural infection with Leishmania braziliensis in two sigmodontines in northeastern Argentina. Parasitology Research. 2018; 117(2):405-412. https://doi.org/10.1007/s00436-017-5714-5 8. Corps B. The effect of graft thickness, donor site and graft-bed on graft shrinkaie in the hooded rat. Br J Plast Surg. 1969; 22:125-133. https://doi.org/10.1016/S0007-1226(69)80053-6 9. Alegre E, De Biasio M, Ramirez N, Ruiz R, Bastiani C. Detección y diferenciación molecular de Leptospiras sp. utilizando diferentes técnicas de extracción de ADN. Rev Vet. 2013; 24(1):53-55. http://dx.doi.org/10.30972/vet.2411151 10. Marcelino A, Ferreira E, Avendanha J, Costa C, Chiarelli D, Almeida G, et al. Molecular detection of Leishmania braziliensis in Rattus norvegicus in an area endemic for cutaneous leishmaniasis in Brazil. Veterinary Parasitology. 2011; 183(1-2):54-58. https://doi.org/10.1016/j.vetpar.2011.06.019 11. Akhavan A, Mirhendi H, Khamesipour A, Alimohammadian M, Rassi Y, Bates P, et al. Leishmania species: detection and identification by nested PCR assay from skin samples of rodent reservoirs. Exp Parasitol. 2010; 126:552–556. https://doi.org/10.1016/j.exppara.2010.06.003 12. de Castro Ferreira E, Cruz I, Cañavate C, de Melo L, Pereira A, Madeira F, et al. Mixed infection of Leishmania infantum and Leishmania braziliensis in rodents from endemic urban area of the New World. BMC Vet Res. 2015; 11(1): 1-7. https://doi.org/10.1186/s12917-015-0392-y 13. Quaresma P, Rêgo F, Botelho H, Silva S, Moura A Jr, Teixeira R. Wild, synanthropic and domestic hosts of Leishmania in an endemic area of cutaneous leishmaniasis in Minas Gerais State, Brazil. Trans R Soc Trop Med Hyg. 2011; 105(10):579-585. http://dx.doi.org/10.1016/j.trstmh.2011.07.005 14. Parhizkari M, Motazedian M, Asgari Q, Mehrabani D. The PCR-based detection of Leishmania major in Mus musculus and other rodents caught in Southern Iran: A guide to sample selection. Ann Trop Med Parasitol. 2011; 105:319–323. https://doi.org/10.1179/136485911X12987676649827 15. Pereira A, Ferreira E, Lima A, Tonelli G, Rêgo F, Paglia A, et al. Detection of Leishmania spp in silvatic mammals and isolation of Leishmania (Viannia) braziliensis from Rattus rattus in an endemic area for leishmaniasis in Minas Gerais State, Brazil. PLoS One. 2017; 12(11):e0187704. https://doi.org/10.1371/journal.pone.0187704 16. Lima B, Dantas-Torres F, Carvalho M, Marinho-Junior J, Almeida E, Brito M. Small mammals as hosts of Leishmania spp. in a highly endemic area for zoonotic leishmaniasis in north-eastern Brazil. Trans R Soc Trop Med Hyg. 2013; 107(9):592-597. http://dx.doi.org/10.1093/trstmh/trt062 17. de Oliveira Lara-Silva F, Andrade Barata R, Monteiro Michalsky E, de Castro Ferreira E, Garcia Lopes M, da Costa Pinheiro A, et al. Rattus norvegicus (Rodentia: Muridae) Infected by Leishmania (Leishmania) infantum (syn. Le. chagasi) in Brazil. Biomed Res Int. 2014; 2014: 1-7. https://doi.org/10.1155/2014/592986 18. Andrade M, Courtenay O, Brito M, Carvalho F, Carvalho A, Soares F, et al. Infectiousness of Sylvatic and Synanthropic Small Rodents Implicates a Multi-host Reservoir of Leishmania (Viannia) braziliensis. PLoS Negl Trop Dis. 2015. 9(10):e0004137. https://doi.org/10.1371/journal.pntd.0004137 https://revistamvz.unicordoba.edu.co/article/view/2546 Derechos de autor 2022 Gabriela Verónica Ramirez, Raquel Mónica Ruiz, Elsa Agustina Alegre https://creativecommons.org/licenses/by-nc-sa/4.0 CC-BY-NC-SA Journal MVZ Cordoba; Vol. 27 No. 3 (2022): Vol. 27 Núm. 3 (2022): Revista MVZ Córdoba Volumen 27(3) Septiembre-Diciembre 2022; e2546 Revista MVZ Córdoba; Vol. 27 Núm. 3 (2022): Vol. 27 Núm. 3 (2022): Revista MVZ Córdoba Volumen 27(3) Septiembre-Diciembre 2022; e2546 1909-0544 0122-0268 10.21897/rmvz.v27.n3.2022 Leishmania DNA epidemiological chain leishmaniasis prevalence black rat polymerase chain reaction ADN de Leishmania Cadena epidemiológica prevalencia rata negra reacción en cadena de la polimerasa info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2022 ftunicordobacojs https://doi.org/10.21897/rmvz.v27.n3.2022 https://doi.org/10.4103/1995-7645.262073 https://doi.org/10.1016/j.ijppaw.2014.08.004 https://doi.org/10.1590/S1984-29612017001 https://doi.org/10.1007/s10393-012-0763-9 https://doi.org/10.4067/S0301-732 2022-11-27T18:57:26Z Objective: the objective of the present work was the detection of Leishmania sp. in Rattus rattus tail base skin through polymerase chain reaction (PCR) techniques. Material and Method: We analyzed 45 Rattus rattus skin samples from the urban area of the city of Corrientes in Argentina. The Leishmania spp detection was performed by nested PCR technique. Results: Leishmania DNA was detected in 22 samples out of 45 processed (49%) in the first round of amplification, and in 14 samples (31%) in the second round (31%). Conclusions: These results contribute to increase the existing information in our region on the possible relationship between Leishmania and Rattus rattus, considering the high prevalence found in skin added to the total absence of lesions. Other aspects should be further studied to establish the role of these animals in the epidemiological chain of the disease in an urban area endemic to leishmaniasis in other animal species. Objetivo: detectar Leishmania sp. en piel de cola de Rattus rattus a través de técnicas de reacción en cadena de la polimerasa. Material y Método: Se analizaron 45 muestras de piel de Rattus rattus del área urbana de la ciudad de Corrientes, Argentina. La detección de Leishmania sp se realizó mediante técnicas de PCR anidada. Resultados: En la primera ronda de amplificación se detectó ADN de Leishmania en 22 muestras de 45 procesadas (49%) y en 14 muestras en la segunda ronda (31%). Conclusiones: Estos resultados contribuyen a aumentar la información existente en nuestra región sobre la posible relación entre Leishmania y Rattus rattus, teniendo en cuenta la alta prevalencia encontrada en piel sumado a la total ausencia de lesiones. Otros aspectos deberán seguir estudiándose para establecer el rol de estos animales en la cadena epidemiológica de la enfermedad en una zona urbana endémica a leishmaniasis en otras especies animales. Article in Journal/Newspaper Rattus rattus Universidad de Córdoba Portal de Revistas Alta Argentina Cadena ENVELOPE(-67.600,-67.600,-67.450,-67.450) Rata ENVELOPE(19.216,19.216,69.928,69.928) Revista MVZ Córdoba 27 3 e2546 |