Identification of bacteria from the Anaplasmataceae family in dog shelter of the municipality of Caldas, Antioquia

Objetive. It was to detect the circulation of microorganisms of the family Anaplasmataceae in a canine albergo of the municipality of Caldas. Materials and methods. In view of the above, a descriptive study was conducted in 46 dogs (Canis lupus familiaris) to evaluate the presence of Ehrlichia spp....

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Published in:Brazilian Journal of Biology
Main Authors: Posada-Zapata, John, Cabrera J, Azucena, González–Alvarez, Dubán, Rodas G, Juan, Monsalve B, Santiago, Londoño B, Andrés
Format: Article in Journal/Newspaper
Language:Spanish
Published: Universidad de Córdoba 2017
Subjects:
Anaplasma
diagnosis
Ehrlichia canis
PCR
serology
diagnóstico
serología
Canis lupus
Online Access:https://revistamvz.unicordoba.edu.co/article/view/1071
id ftunicordobacojs:oai:ojs.revistas.unicordoba.edu.co:article/1071
record_format openpolar
institution Open Polar
collection Universidad de Córdoba Portal de Revistas
op_collection_id ftunicordobacojs
language Spanish
topic Anaplasma
diagnosis
Ehrlichia canis
PCR
serology
diagnóstico
serología
spellingShingle Anaplasma
diagnosis
Ehrlichia canis
PCR
serology
diagnóstico
serología
Posada-Zapata, John
Cabrera J, Azucena
González–Alvarez, Dubán
Rodas G, Juan
Monsalve B, Santiago
Londoño B, Andrés
Identification of bacteria from the Anaplasmataceae family in dog shelter of the municipality of Caldas, Antioquia
topic_facet Anaplasma
diagnosis
Ehrlichia canis
PCR
serology
diagnóstico
serología
description Objetive. It was to detect the circulation of microorganisms of the family Anaplasmataceae in a canine albergo of the municipality of Caldas. Materials and methods. In view of the above, a descriptive study was conducted in 46 dogs (Canis lupus familiaris) to evaluate the presence of Ehrlichia spp. and Anaplasma spp. by molecular techniques or by immunodiagnostic, in a dog shelter of the municipality of Caldas. The DNA extraction was done in whole blood samples with EDTA. Primers were used against dsb and groEL genes to detect Ehrlichia and Anaplasma sequences respectively and phylogenetic analyzes of the sequences obtained were also carried out. On the other hand, SNAP 4DX® was used for serodiagnosis. Results. In the molecular testing, five positive samples were obtained for the Ehrlichia test and three for the Anaplasma test. In the serological tests, three individuals were positive for Ehrlichia spp., three for Anaplasma spp. and for the other trials measured by the SNAP (Dirofilaria immitis y Borrelia burgdorfe) the result was negative. Conclusions. It demonstrated the circulation of Ehrlichia canis and Anaplasma platys in a dog shelter of the municipality of Caldas, Antioquia. Objetivo. Fue detectar la circulación de microorganismos de la familia Anaplasmataceae en un alberge canino del municipio de Caldas. Materiales y métodos. Se realizó un estudio descriptivo en 46 perros (Canis lupus familiaris) para evaluar por técnicas moleculares o por inmunodiagnóstico la presencia de Ehrlichia spp. y Anaplasma spp., en un albergue canino del município de Caldas. Se hizo extracción de ADN en muestras de sangre entera con EDTA y se utilizaron cebadores contra los genes dsb y groEl, para detectar secuencias de Ehrlichia y Anaplasma respectivamente y análisis filogenéticos de las secuencias obtenidas. Por otro lado se usó el SNAP 4DX® para el serodiagnóstico. Resultados. En las pruebas moleculares se obtuvieron cinco muestras positivas para Ehrlichia y tres para Anamaplasma. En las pruebas serológicas, tres ...
format Article in Journal/Newspaper
author Posada-Zapata, John
Cabrera J, Azucena
González–Alvarez, Dubán
Rodas G, Juan
Monsalve B, Santiago
Londoño B, Andrés
author_facet Posada-Zapata, John
Cabrera J, Azucena
González–Alvarez, Dubán
Rodas G, Juan
Monsalve B, Santiago
Londoño B, Andrés
author_sort Posada-Zapata, John
title Identification of bacteria from the Anaplasmataceae family in dog shelter of the municipality of Caldas, Antioquia
title_short Identification of bacteria from the Anaplasmataceae family in dog shelter of the municipality of Caldas, Antioquia
title_full Identification of bacteria from the Anaplasmataceae family in dog shelter of the municipality of Caldas, Antioquia
title_fullStr Identification of bacteria from the Anaplasmataceae family in dog shelter of the municipality of Caldas, Antioquia
title_full_unstemmed Identification of bacteria from the Anaplasmataceae family in dog shelter of the municipality of Caldas, Antioquia
title_sort identification of bacteria from the anaplasmataceae family in dog shelter of the municipality of caldas, antioquia
publisher Universidad de Córdoba
publishDate 2017
url https://revistamvz.unicordoba.edu.co/article/view/1071
genre Canis lupus
genre_facet Canis lupus
op_source Journal MVZ Cordoba; Vol. 22 No. supl (2017): Revista MVZ Córdoba Volumen 22(Supl) Mayo 2017; 6014-6022
Revista MVZ Córdoba; Vol. 22 Núm. supl (2017): Revista MVZ Córdoba Volumen 22(Supl) Mayo 2017; 6014-6022
1909-0544
0122-0268
op_relation https://revistamvz.unicordoba.edu.co/article/view/1071/pdf
1. Hidrón BA, Mu-oz RF, Vega MJ. Primer caso de ehrlichiosis monocítica humana reportado en Colombia. Infectio 2014; 18(4):162-166. https://doi.org/10.1016/j.infect.2014.04.001
2. Openshaw JJ, Swerdlow DL. Human ehrlichiosis: clinical and ecological challenges. South Med J 2007; 100(8):769–70. https://doi.org/10.1097/SMJ.0b013e318063ebb5
3. Little SE. Ehrlichiosis. Arthropod Borne Diseases. Springer International Publishing; Switzerland: 2017.
4. Dolz G, Ábrego L, Romero LE, Campos-Calderón L, Bouza-Mora L, Jiménez-Rocha AE. Ehrlichiosis and anaplasmosis in Costa Rica. Acta Med Costarric 2013; 55(Supl).
5. Nair ADS, Cheng C, Ganta CK, Sanderson MW, Alleman R, Munderloh UG, et al. Comparative Experimental Infection Study in Dogs with Ehrlichia canis, E. chaffeensis, Anaplasma platys and A. phagocytophilum. PLoS One 2016; 11(2):1-21. https://doi.org/10.1371/journal.pone.0148239
6. Vargas-Hernandez G, André MR, Cendales DM, Sousa KC, Gonçalves MD, Rondelli LR, et al. Molecular detection of Anaplasma species in dogs in Colombia. Braz J Vet Parasitol 2016; 25(4):459–64. https://doi.org/10.1590/s1984-29612016066
7. Miranda J, Mattar S. Molecular detection of Anaplasma sp. and ehrlichia sp. in ticks collected in domestical animals, Colombia. Trop Biomed 2015; 32(4):726–35.
8. Lina María CY, Leonardo Alberto RO, Jaiberth Antonio CA. Seroprevalencia de Ehrlichia canis en perros con sospecha de infección por patógenos transmitidos por garrapatas en Medellín, 2012-2014. Rev Med Vet 2015; (29):51–62.
9. Labruna MB, McBride JW, Camargo LMA, Aguiar DM, Yabsley MJ, Davidson WR, et al. A preliminary investigation of Ehrlichia species in ticks, humans, dogs, and capybaras from Brazil. Vet Parasitol 2007; 143(2):189–95. https://doi.org/10.1016/j.vetpar.2006.08.005
10. Inokuma H, Fujii K, Okuda M, Onishi T, Beaufils J, Raoult D, et al. Determination of the Nucleotide Sequences of Heat Shock Operon. Clin Diagn Lab Immunol. 2002; 9(5):1132–1136.
11. Cicuttin GL, Tarragona EL, De Salvo MN, Mangold AJ, Nava S. Infection with Ehrlichia canis and Anaplasma platys (Rickettsiales: Anaplasmataceae) in two lineages of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) from Argentina. Ticks Tick Borne Dis 2015; 6(6):724–9. https://doi.org/10.1016/j.ttbdis.2015.06.006
12. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 2007; 24(8):1596–9. https://doi.org/10.1093/molbev/msm092
13. O'Connor TP. SNAP Assay Technology. Top Companion Anim Med 2015; 30(4):132–8. https://doi.org/10.1053/j.tcam.2015.12.002
14. Bossa-Miranda MA, Valencia-Celis V del C, Carvajal-Giraldo BA, Ríos-Osorio LA. Automated hemogram values for healthy dogs aged 1 to 6 years attended at the Veterinary Hospital - Universidad de Antioquia (Colombia), 2002-2009. Rev Colomb Ciencias Pecu. 2012; 25(3):409–16.
15. Rojas A, Rueda A, Díaz D, Mesa NC, Benavides J, Imbachi K, et al. Identificación de Ehrlichia canis (Donatien & Lestoquard) Moshkovski mediante PCR anidada. Vet Zootec 2013; 7(1):37–48.
16. Salazar H, Buriticá EF, Echeverry DF, Barbosa IX. Seroprevalencia de Ehrlichia canis y su relación con algunos parámetros clínicos y hematológicos en caninos admitidos en clínicas veterinarias de la ciudad de Ibagué (Colombia). Rev Colomb Cienc Anim 2014; 7(1):56-63.
17. Harrus S, Waner T. Diagnosis of canine monocytotropic ehrlichiosis (Ehrlichia canis): An overview. Vet J 2011; 187(3):292–6. DOI: http://dx.doi.org/10.1016/j.tvjl.2010.02.001
18. Wong SSY, Teng JLL, Poon RWS, Choi GKY, Chan K-H, Yeung ML, et al. Comparative Evaluation of a Point-of-Care Immunochromatographic Test SNAP 4Dx with Molecular Detection Tests for Vector-Borne Canine Pathogens in Hong Kong. Vector-Borne Zoonotic Dis 2011; 11(9):1269–77. https://doi.org/10.1089/vbz.2010.0265
https://revistamvz.unicordoba.edu.co/article/view/1071
op_doi https://doi.org/10.1016/j.infect.2014.04.001
https://doi.org/10.1097/SMJ.0b013e318063ebb5
https://doi.org/10.1371/journal.pone.0148239
https://doi.org/10.1590/s1984-29612016066
https://doi.org/10.1016/j.vetpar.2006.08.005
https://doi.org/10.1016
container_title Brazilian Journal of Biology
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container_issue 4
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spelling ftunicordobacojs:oai:ojs.revistas.unicordoba.edu.co:article/1071 2023-05-15T15:50:12+02:00 Identification of bacteria from the Anaplasmataceae family in dog shelter of the municipality of Caldas, Antioquia Identificación de bacterias de la familia Anaplasmataceae en un albergue canino del municipio de Caldas, Antioquia Posada-Zapata, John Cabrera J, Azucena González–Alvarez, Dubán Rodas G, Juan Monsalve B, Santiago Londoño B, Andrés 2017-05-25 application/pdf https://revistamvz.unicordoba.edu.co/article/view/1071 spa spa Universidad de Córdoba https://revistamvz.unicordoba.edu.co/article/view/1071/pdf 1. Hidrón BA, Mu-oz RF, Vega MJ. Primer caso de ehrlichiosis monocítica humana reportado en Colombia. Infectio 2014; 18(4):162-166. https://doi.org/10.1016/j.infect.2014.04.001 2. Openshaw JJ, Swerdlow DL. Human ehrlichiosis: clinical and ecological challenges. South Med J 2007; 100(8):769–70. https://doi.org/10.1097/SMJ.0b013e318063ebb5 3. Little SE. Ehrlichiosis. Arthropod Borne Diseases. Springer International Publishing; Switzerland: 2017. 4. Dolz G, Ábrego L, Romero LE, Campos-Calderón L, Bouza-Mora L, Jiménez-Rocha AE. Ehrlichiosis and anaplasmosis in Costa Rica. Acta Med Costarric 2013; 55(Supl). 5. Nair ADS, Cheng C, Ganta CK, Sanderson MW, Alleman R, Munderloh UG, et al. Comparative Experimental Infection Study in Dogs with Ehrlichia canis, E. chaffeensis, Anaplasma platys and A. phagocytophilum. PLoS One 2016; 11(2):1-21. https://doi.org/10.1371/journal.pone.0148239 6. Vargas-Hernandez G, André MR, Cendales DM, Sousa KC, Gonçalves MD, Rondelli LR, et al. Molecular detection of Anaplasma species in dogs in Colombia. Braz J Vet Parasitol 2016; 25(4):459–64. https://doi.org/10.1590/s1984-29612016066 7. Miranda J, Mattar S. Molecular detection of Anaplasma sp. and ehrlichia sp. in ticks collected in domestical animals, Colombia. Trop Biomed 2015; 32(4):726–35. 8. Lina María CY, Leonardo Alberto RO, Jaiberth Antonio CA. Seroprevalencia de Ehrlichia canis en perros con sospecha de infección por patógenos transmitidos por garrapatas en Medellín, 2012-2014. Rev Med Vet 2015; (29):51–62. 9. Labruna MB, McBride JW, Camargo LMA, Aguiar DM, Yabsley MJ, Davidson WR, et al. A preliminary investigation of Ehrlichia species in ticks, humans, dogs, and capybaras from Brazil. Vet Parasitol 2007; 143(2):189–95. https://doi.org/10.1016/j.vetpar.2006.08.005 10. Inokuma H, Fujii K, Okuda M, Onishi T, Beaufils J, Raoult D, et al. Determination of the Nucleotide Sequences of Heat Shock Operon. Clin Diagn Lab Immunol. 2002; 9(5):1132–1136. 11. Cicuttin GL, Tarragona EL, De Salvo MN, Mangold AJ, Nava S. Infection with Ehrlichia canis and Anaplasma platys (Rickettsiales: Anaplasmataceae) in two lineages of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) from Argentina. Ticks Tick Borne Dis 2015; 6(6):724–9. https://doi.org/10.1016/j.ttbdis.2015.06.006 12. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 2007; 24(8):1596–9. https://doi.org/10.1093/molbev/msm092 13. O'Connor TP. SNAP Assay Technology. Top Companion Anim Med 2015; 30(4):132–8. https://doi.org/10.1053/j.tcam.2015.12.002 14. Bossa-Miranda MA, Valencia-Celis V del C, Carvajal-Giraldo BA, Ríos-Osorio LA. Automated hemogram values for healthy dogs aged 1 to 6 years attended at the Veterinary Hospital - Universidad de Antioquia (Colombia), 2002-2009. Rev Colomb Ciencias Pecu. 2012; 25(3):409–16. 15. Rojas A, Rueda A, Díaz D, Mesa NC, Benavides J, Imbachi K, et al. Identificación de Ehrlichia canis (Donatien & Lestoquard) Moshkovski mediante PCR anidada. Vet Zootec 2013; 7(1):37–48. 16. Salazar H, Buriticá EF, Echeverry DF, Barbosa IX. Seroprevalencia de Ehrlichia canis y su relación con algunos parámetros clínicos y hematológicos en caninos admitidos en clínicas veterinarias de la ciudad de Ibagué (Colombia). Rev Colomb Cienc Anim 2014; 7(1):56-63. 17. Harrus S, Waner T. Diagnosis of canine monocytotropic ehrlichiosis (Ehrlichia canis): An overview. Vet J 2011; 187(3):292–6. DOI: http://dx.doi.org/10.1016/j.tvjl.2010.02.001 18. Wong SSY, Teng JLL, Poon RWS, Choi GKY, Chan K-H, Yeung ML, et al. Comparative Evaluation of a Point-of-Care Immunochromatographic Test SNAP 4Dx with Molecular Detection Tests for Vector-Borne Canine Pathogens in Hong Kong. Vector-Borne Zoonotic Dis 2011; 11(9):1269–77. https://doi.org/10.1089/vbz.2010.0265 https://revistamvz.unicordoba.edu.co/article/view/1071 Journal MVZ Cordoba; Vol. 22 No. supl (2017): Revista MVZ Córdoba Volumen 22(Supl) Mayo 2017; 6014-6022 Revista MVZ Córdoba; Vol. 22 Núm. supl (2017): Revista MVZ Córdoba Volumen 22(Supl) Mayo 2017; 6014-6022 1909-0544 0122-0268 Anaplasma diagnosis Ehrlichia canis PCR serology diagnóstico serología info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2017 ftunicordobacojs https://doi.org/10.1016/j.infect.2014.04.001 https://doi.org/10.1097/SMJ.0b013e318063ebb5 https://doi.org/10.1371/journal.pone.0148239 https://doi.org/10.1590/s1984-29612016066 https://doi.org/10.1016/j.vetpar.2006.08.005 https://doi.org/10.1016 2022-11-07T09:30:05Z Objetive. It was to detect the circulation of microorganisms of the family Anaplasmataceae in a canine albergo of the municipality of Caldas. Materials and methods. In view of the above, a descriptive study was conducted in 46 dogs (Canis lupus familiaris) to evaluate the presence of Ehrlichia spp. and Anaplasma spp. by molecular techniques or by immunodiagnostic, in a dog shelter of the municipality of Caldas. The DNA extraction was done in whole blood samples with EDTA. Primers were used against dsb and groEL genes to detect Ehrlichia and Anaplasma sequences respectively and phylogenetic analyzes of the sequences obtained were also carried out. On the other hand, SNAP 4DX® was used for serodiagnosis. Results. In the molecular testing, five positive samples were obtained for the Ehrlichia test and three for the Anaplasma test. In the serological tests, three individuals were positive for Ehrlichia spp., three for Anaplasma spp. and for the other trials measured by the SNAP (Dirofilaria immitis y Borrelia burgdorfe) the result was negative. Conclusions. It demonstrated the circulation of Ehrlichia canis and Anaplasma platys in a dog shelter of the municipality of Caldas, Antioquia. Objetivo. Fue detectar la circulación de microorganismos de la familia Anaplasmataceae en un alberge canino del municipio de Caldas. Materiales y métodos. Se realizó un estudio descriptivo en 46 perros (Canis lupus familiaris) para evaluar por técnicas moleculares o por inmunodiagnóstico la presencia de Ehrlichia spp. y Anaplasma spp., en un albergue canino del município de Caldas. Se hizo extracción de ADN en muestras de sangre entera con EDTA y se utilizaron cebadores contra los genes dsb y groEl, para detectar secuencias de Ehrlichia y Anaplasma respectivamente y análisis filogenéticos de las secuencias obtenidas. Por otro lado se usó el SNAP 4DX® para el serodiagnóstico. Resultados. En las pruebas moleculares se obtuvieron cinco muestras positivas para Ehrlichia y tres para Anamaplasma. En las pruebas serológicas, tres ... Article in Journal/Newspaper Canis lupus Universidad de Córdoba Portal de Revistas Brazilian Journal of Biology 68 4 771 779

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