Plant cell cultures of Nordic berry species: Phenolic and carotenoid profiling and biological assessments

• Published in: Food Chemistry
• Main Authors: Rocchetti G., Lucini L.
• Other Authors: Rischer, H., Nohynek, L., Puupponen-Pimia, R., Aguiar, J., Rocchetti, Gabriele, Lucini, Luigi, Camara, J. S., Mendanha Cruz, T., Boscacci Marques, M., Granato, D.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier Ltd 2022
• Subjects: Antiproliferative activity; Food innovation; Food toxicology; Free radical scavengers; Functional foods; LC-MS; Phenolic composition; Antioxidants; Carotenoids; Cell Culture Techniques; Humans; Plant Extracts; Fruit; Phenols; Settore AGR/13 - CHIMICA AGRARIA; Settore CHIM/10 - CHIMICA DEGLI ALIMENTI; Arctic
• Online Access: http://hdl.handle.net/10807/197997; https://doi.org/10.1016/j.foodchem.2021.130571

Plant cell cultures from cloudberry (CL), lingonberry (LI), stone berry (ST), arctic bramble (AB), and strawberry (SB) were studied in terms of their polyphenol and carotenoid composition, antioxidant activity, antihemolytic activity and cytotoxicity effects on cancerous cells. High-resolution mass spectrometry data showed that LI, presented the highest antioxidant activity, contained the highest contents of flavones, phenolic acids, lignans, and total carotenoids, while CL, ST and SB presented the opposite behavior. AB and SB presented the lowest FRAP and CUPRAC values, while AB and CL presented the lowest reducing power. SB presented the lowest antioxidant activity measured by single electron transfer assays and the lowest content of lignans, phenolic acids, and flavones. CL and LI decreased the viability of in vitro mammary gland adenocarcinoma while only LI decreased the viability of in vitro lung carcinoma and showed protective effects of human erythrocytes against mechanical hemolysis.