Biocleaning of acrylic materials
In a restoration, conservation and enhancement of Cultural Heritage project, the cleanup phase is one of the earliest and most delicate operations for the removal of dirt, black crusts, consolidating, protective coatings used in past restorations or, finally, of spray paints. The goal of this study...
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Editorial Universitat Politécnica de València
2014
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Online Access: | http://hdl.handle.net/11584/91880 https://doi.org/10.4995/EMERGE2014.2014.539 |
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ftunicagliariris:oai:iris.unica.it:11584/91880 2024-02-11T09:58:09+01:00 Biocleaning of acrylic materials Germinario G van der Werf ID Montes Estellés RM Regidor Ros JL SABBATINI, LUIGIA M.V. Vivancos, M.T. Doménech, M. Sánchez Pons, J. Osca Pons Germinario, G van der Werf, Id Montes Estellés, Rm Regidor Ros, Jl Sabbatini, Luigia 2014 http://hdl.handle.net/11584/91880 https://doi.org/10.4995/EMERGE2014.2014.539 spa eng spa eng Editorial Universitat Politécnica de València place:Valencia info:eu-repo/semantics/altIdentifier/isbn/978-84-9048-317-6 ispartofbook:Jornadas de Investigación Emergente en Conservación y Restauración de Patrimonio EMERGE2014 firstpage:39 lastpage:46 numberofpages:8 http://hdl.handle.net/11584/91880 http://dx.doi.org/10.4995/EMERGE2014.2014.539 lipase micro-Raman acrylic biocleaning FTIR Py-GC/MS info:eu-repo/semantics/conferenceObject 2014 ftunicagliariris https://doi.org/10.4995/EMERGE2014.2014.539 2024-01-17T17:47:23Z In a restoration, conservation and enhancement of Cultural Heritage project, the cleanup phase is one of the earliest and most delicate operations for the removal of dirt, black crusts, consolidating, protective coatings used in past restorations or, finally, of spray paints. The goal of this study has been to verify the effectiveness of an environmentally sustainable biocleaning method using enzymes, such as lipases, for removing acrylic-based materials. The lipases purchased are extracted from fungi such as Candida rugosa (Type VII Sigma-Aldrich) and as Candida Antarctica (Sigma Aldrich), and various bacteria in a mix of lipase (CTS), and then applied on the specimens specifically prepared. The specimens are white ceramic substrates with a thin layer of different spray paints, markers and Paraloid, all based on acrylic. These materials were analyzed by Py-GC/MS, micro-Raman and FTIR spectroscopy in order to know the chemical composition of the formulations. Usually the spray paints and markers compositions are more complex because they consist of a binder, dyes and/or pigments and additives that confer to paint particular physico-chemical properties. The lipase action should hydrolyze the ester bond of the acrylic copolymers, transforming them from hydrophobic to hydrophilic compounds, facilitating their removal. The enzymes have been applied directly on the test specimens by employing both simple aqueous systems, or direct microemulsions in different conditions of time and temperature. The optimum activity of each lipase considered (pH and substrate concentration) was studied spectrophotometrically on a standard substrate. Conference Object Antarc* Antarctica Università degli Studi di Cagliari: UNICA IRIS Aldrich ENVELOPE(158.217,158.217,-80.117,-80.117) Rugosa ENVELOPE(-61.250,-61.250,-62.633,-62.633) Emerge2014. Jornadas de Investigación Emergente en Conservación y Restauración de Patrimonio 1 1076 |
institution |
Open Polar |
collection |
Università degli Studi di Cagliari: UNICA IRIS |
op_collection_id |
ftunicagliariris |
language |
Spanish English |
topic |
lipase micro-Raman acrylic biocleaning FTIR Py-GC/MS |
spellingShingle |
lipase micro-Raman acrylic biocleaning FTIR Py-GC/MS Germinario G van der Werf ID Montes Estellés RM Regidor Ros JL SABBATINI, LUIGIA Biocleaning of acrylic materials |
topic_facet |
lipase micro-Raman acrylic biocleaning FTIR Py-GC/MS |
description |
In a restoration, conservation and enhancement of Cultural Heritage project, the cleanup phase is one of the earliest and most delicate operations for the removal of dirt, black crusts, consolidating, protective coatings used in past restorations or, finally, of spray paints. The goal of this study has been to verify the effectiveness of an environmentally sustainable biocleaning method using enzymes, such as lipases, for removing acrylic-based materials. The lipases purchased are extracted from fungi such as Candida rugosa (Type VII Sigma-Aldrich) and as Candida Antarctica (Sigma Aldrich), and various bacteria in a mix of lipase (CTS), and then applied on the specimens specifically prepared. The specimens are white ceramic substrates with a thin layer of different spray paints, markers and Paraloid, all based on acrylic. These materials were analyzed by Py-GC/MS, micro-Raman and FTIR spectroscopy in order to know the chemical composition of the formulations. Usually the spray paints and markers compositions are more complex because they consist of a binder, dyes and/or pigments and additives that confer to paint particular physico-chemical properties. The lipase action should hydrolyze the ester bond of the acrylic copolymers, transforming them from hydrophobic to hydrophilic compounds, facilitating their removal. The enzymes have been applied directly on the test specimens by employing both simple aqueous systems, or direct microemulsions in different conditions of time and temperature. The optimum activity of each lipase considered (pH and substrate concentration) was studied spectrophotometrically on a standard substrate. |
author2 |
M.V. Vivancos, M.T. Doménech, M. Sánchez Pons, J. Osca Pons Germinario, G van der Werf, Id Montes Estellés, Rm Regidor Ros, Jl Sabbatini, Luigia |
format |
Conference Object |
author |
Germinario G van der Werf ID Montes Estellés RM Regidor Ros JL SABBATINI, LUIGIA |
author_facet |
Germinario G van der Werf ID Montes Estellés RM Regidor Ros JL SABBATINI, LUIGIA |
author_sort |
Germinario G |
title |
Biocleaning of acrylic materials |
title_short |
Biocleaning of acrylic materials |
title_full |
Biocleaning of acrylic materials |
title_fullStr |
Biocleaning of acrylic materials |
title_full_unstemmed |
Biocleaning of acrylic materials |
title_sort |
biocleaning of acrylic materials |
publisher |
Editorial Universitat Politécnica de València |
publishDate |
2014 |
url |
http://hdl.handle.net/11584/91880 https://doi.org/10.4995/EMERGE2014.2014.539 |
long_lat |
ENVELOPE(158.217,158.217,-80.117,-80.117) ENVELOPE(-61.250,-61.250,-62.633,-62.633) |
geographic |
Aldrich Rugosa |
geographic_facet |
Aldrich Rugosa |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/isbn/978-84-9048-317-6 ispartofbook:Jornadas de Investigación Emergente en Conservación y Restauración de Patrimonio EMERGE2014 firstpage:39 lastpage:46 numberofpages:8 http://hdl.handle.net/11584/91880 http://dx.doi.org/10.4995/EMERGE2014.2014.539 |
op_doi |
https://doi.org/10.4995/EMERGE2014.2014.539 |
container_title |
Emerge2014. Jornadas de Investigación Emergente en Conservación y Restauración de Patrimonio |
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