Genome sequence analysis of Pseudomonas extremaustralis provides new insights into environmental adaptability and extreme conditions resistance

• Main Authors: Raiger Iustman, L.J., Tribelli, P.M., Ibarra, J.G., Catone, M.V., Solar Venero, E.C., López, N.I.
• Format: Journal/Newspaper
• Language: unknown
• Subjects: Environmental adaptability; Exopolysaccharides; Genome analysis; Horizontal transfer; Microaerobic metabolism; Pseudomonas; Bacteria (microorganisms); acetate kinase; adenosine triphosphatase; alginic acid; arginine; colanic acid; coumaric acid; ferulic acid; polysaccharide; pyruvic acid derivative; trehalose; adaptation; Antarctica; bacterial genome; biology; chemistry; cold; DNA sequence; environment; fermentation; genetics; metabolism; osmosis; phenotype; phylogeny; physiology; Biological; Adenosine Triphosphatases; Alginates; Antarctic Regions; Cold Temperature; Computational Biology; Coumaric Acids; Genome; Bacterial; Antarc*; Antarctic
• Online Access: https://hdl.handle.net/20.500.12110/paper_14310651_v19_n1_p207_RaigerIustman

The genome of the Antarctic bacterium Pseudomonas extremaustralis was analyzed searching for genes involved in environmental adaptability focusing on anaerobic metabolism, osmoregulation, cold adaptation, exopolysaccharide production and degradation of complex compounds. Experimental evidences demonstrated the functionality of several of these pathways, including arginine and pyruvate fermentation, alginate production and growth under cold conditions. Phylogenetic analysis along with genomic island prediction allowed the detection of genes with probable foreign origin such as those coding for acetate kinase, osmotic resistance and colanic acid biosynthesis. These findings suggest that in P. extremaustralis the horizontal transfer events and/or gene redundancy could play a key role in the survival under unfavorable conditions. Comparative genome analysis of these traits in other representative Pseudomonas species highlighted several similarities and differences with this extremophile bacterium. © 2014, Springer Japan. Fil:Raiger Iustman, L.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tribelli, P.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Catone, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.