Biofilm lifestyle enhances diesel bioremediation and biosurfactant production in the Antarctic polyhydroxyalkanoate producer Pseudomonas extremaustralis

• Main Authors: Tribelli, P.M., Di Martino, C., López, N.I., Raiger Iustman, L.J.
• Format: Journal/Newspaper
• Language: unknown
• Subjects: Biofilm; Biosurfactant production; Diesel bioremediation; Polyhydroxyalkanoates; Pseudomonas; Antarctic bacteria; Biofilm cells; Branched alkanes; Contaminated environment; Extreme environment; Flask culture; Hydrocarbon bioremediation; Inocula; Poly-hydroxyalkanoate; Bacteria; Biomolecules; Bioremediation; Biotechnology; Bottles; Cell culture; Cell growth; Degradation; Growth kinetics; Paraffins; Pollution; Surface active agents; Biofilms; alkane; carbon; gasoline; polyhydroxyalkanoic acid; surfactant; bacterium; biogenic material; cytology; diesel; ester; industrial production; microbial activity; pollution tolerance; polymer; survivorship; Antarc*; Antarctic
• Online Access: https://hdl.handle.net/20.500.12110/paper_09239820_v23_n5_p645_Tribelli

Diesel is a widely distributed pollutant. Bioremediation of this kind of compounds requires the use of microorganisms able to survive and adapt to contaminated environments. Pseudomonas extremaustralis is an Antarctic bacterium with a remarkable survival capability associated to polyhydroxyalkanoates (PHAs) production. This strain was used to investigate the effect of cell growth conditions-in biofilm versus shaken flask cultures-as well as the inocula characteristics associated with PHAs accumulation, on diesel degradation. Biofilms showed increased cell growth, biosurfactant production and diesel degradation compared with that obtained in shaken flask cultures. PHA accumulation decreased biofilm cell attachment and enhanced biosurfactant production. Degradation of long-chain and branched alkanes was observed in biofilms, while in shaken flasks only medium-chain length alkanes were degraded. This work shows that the PHA accumulating bacterium P. extremaustralis can be a good candidate to be used as hydrocarbon bioremediation agent, especially in extreme environments. © 2012 Springer Science+Business Media B.V. Fil:Tribelli, P.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Raiger Iustman, L.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.