Structure and Cell Type Distribution in the Pituitary Gland of Pejerrey Odontesthes bonariensis

• Main Authors: Vissio, P.G., Somoza, G.M., Maggese, M.C., Paz, D.A., Strüssmann, C.A.
• Format: Journal/Newspaper
• Language: unknown
• Subjects: Immunocytochemistry; Odontesthes bonariensis; Pejerrey; Pituitary gland; Pituitary hormones; Cyprinus carpio; Mammalia; Oncorhynchus keta; Pisces; Sciaenops ocellatus; Red drum
• Online Access: https://hdl.handle.net/20.500.12110/paper_09199268_v63_n1_p64_Vissio

Antisera raised against piscine pituitary hormones and heterologous antisera against mammalian pituitary hormones were used to analyze the types and distribution patterns of cells in the adenohypophysis of pejerrey Odontesthes bonariensis. Prolactin (PRL) cells were immunostained in the external border of the rostral pars distalis using anti-chum salmon PRL and anti-carp PRL. Immunoreactive (ir-)corticotrophic (ACTH) cells were identified in the same area using anti-human ACTH and cross-reactivity was observed in the pars intermedia (PI), corresponding to the presumptive melanocyte-stimulating hormone cells. Ir-growth hormone (GH) cells were identified in close contact with the neurohypophysis in the proximal pars distalis (PPD) using anti-chum salmon GH and anti-carp GH antisera. Ir-gonadotrophic (GtH) cells were distributed in the PPD and in the external area of the PI. Two forms of GtH were identified using antisera raised against chum salmon GtH Iβ and GtH IIβ and anti-croaker maturational GtH. Ir-thyroid-stimulating hormone (TSH) cells were revealed with an anti-human TSHβ antiserum in the PPD. Ir-somatolactin (SL) cells were detected in the PI using two antisera raised against chum salmon and red drum SL.