Solution and crystal structure of BA42, a protein from the Antarctic bacterium Bizionia argentinensis comprised of a stand-alone TPM domain

• Main Authors: Aran, M., Smal, C., Pellizza, L., Gallo, M., Otero, L.H., Klinke, S., Goldbaum, F.A., Ithurralde, E.R., Bercovich, A., Mac Cormack, W.P., Turjanski, A.G., Cicero, D.O.
• Format: Journal/Newspaper
• Language: unknown
• Subjects: Antarctic bacteria; BA42; Bizionia argentinensis; Nuclear magnetic resonance; Protein structure; Structural genomics; X-ray crystallography; ba42 protein; bacterial protein; metal ion; unclassified drug; calcium; metal; Article; beta sheet; binding affinity; binding site; crystal structure; Flavobacterium; metal binding; molecular dynamics; nonhuman; priority journal; protein domain; protein family; protein stability; X ray crystallography; amino acid sequence; animal; Antarctica; chemical structure; chemistry; circular dichroism; Flavobacteriaceae; genetics; metabolism; molecular genetics; protein conformation; protein tertiary structure; sequence homology; Bacteria (microorganisms); Bizionia; Animals; Antarctic Regions; Bacterial Proteins; Antarc*; Antarctic
• Online Access: https://hdl.handle.net/20.500.12110/paper_08873585_v82_n11_p3062_Aran

The structure of the BA42 protein belonging to the Antarctic flavobacterium Bizionia argentinensis was determined by nuclear magnetic resonance and X-ray crystallography. This is the first structure of a member of the PF04536 family comprised of a stand-alone TPM domain. The structure reveals a new topological variant of the four β-strands constituting the central β-sheet of the αβα architecture and a double metal binding site stabilizing a pair of crossing loops, not observed in previous structures of proteins belonging to this family. BA42 shows differences in structure and dynamics in the presence or absence of bound metals. The affinity for divalent metal ions is close to that observed in proteins that modulate their activity as a function of metal concentration, anticipating a possible role for BA42. © 2014 Wiley Periodicals, Inc. Fil:Aran, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Smal, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gallo, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Klinke, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mac Cormack, W.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Turjanski, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cicero, D.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.