Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status

The bacterioplankton assemblages of eight maritime Antarctic lakes with a wide range of trophic status and geographic span (six lakes from Hope Bay, Antarctic Peninsula and two from Potter Peninsula, King George Island) were described using denaturing gradient gel electrophoresis and band sequencing...

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Bibliographic Details
Main Authors: Schiaffino, M.R., Unrein, F., Gasol, J.M., Farias, M.E., Estevez, C., Balagué, V., Izaguirre, I.
Format: Journal/Newspaper
Language:unknown
Subjects:
gel
RNA
Online Access:https://hdl.handle.net/20.500.12110/paper_07224060_v32_n6_p923_Schiaffino
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record_format openpolar
spelling ftunibueairesbd:todo:paper_07224060_v32_n6_p923_Schiaffino 2023-10-29T02:32:28+01:00 Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status Schiaffino, M.R. Unrein, F. Gasol, J.M. Farias, M.E. Estevez, C. Balagué, V. Izaguirre, I. https://hdl.handle.net/20.500.12110/paper_07224060_v32_n6_p923_Schiaffino unknown http://hdl.handle.net/20.500.12110/paper_07224060_v32_n6_p923_Schiaffino info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar 16S rRNA gene Bacterioplankton Denaturing gradient gel electrophoresis Hope Bay (Antarctic Peninsula) Maritime Antarctic lakes Potter Peninsula (King George Island) bacterium cluster analysis comparative study cyanobacterium dominance electrokinesis gel gene microbial community RNA trophic status Antarctic Peninsula Antarctica Hope Bay King George Island Potter Peninsula South Shetland Islands West Antarctica Actinobacteria Bacteroidetes Betaproteobacteria Cyanobacteria JOUR ftunibueairesbd https://doi.org/20.500.12110/paper_07224060_v32_n6_p923_Schiaffino 2023-10-05T01:48:55Z The bacterioplankton assemblages of eight maritime Antarctic lakes with a wide range of trophic status and geographic span (six lakes from Hope Bay, Antarctic Peninsula and two from Potter Peninsula, King George Island) were described using denaturing gradient gel electrophoresis and band sequencing during two consecutive austral summers (2003-2004). Analyses of the gels identified a total of 230 bands spread across 57 different positions. Among those bands, 14 were shared between lakes from Hope Bay and Potter Peninsula, 17 were observed only in particular lakes, and 17 were registered both years in the same lake. We successfully reamplified and sequenced 43 bands located in 36 different positions belonging to Bacteroidetes, Actinobacteria, Betaproteobacteria and Cyanobacteria. The closest matches for 63% of the sequenced bands were from Antarctic or from other cold environment clones and sequences already in the databases, suggesting the widespread dominance of microbial communities adapted to cold habitats. The results of the multivariate analyses (Cluster Analysis and CCA) indicated that the nutrient status of the lake influences the bacterioplankton assemblages. © 2009 Springer-Verlag. Fil:Schiaffino, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Unrein, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Izaguirre, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica King George Island South Shetland Islands West Antarctica Biblioteca Digital FCEN-UBA (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires)
institution Open Polar
collection Biblioteca Digital FCEN-UBA (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires)
op_collection_id ftunibueairesbd
language unknown
topic 16S rRNA gene
Bacterioplankton
Denaturing gradient gel electrophoresis
Hope Bay (Antarctic Peninsula)
Maritime Antarctic lakes
Potter Peninsula (King George Island)
bacterium
cluster analysis
comparative study
cyanobacterium
dominance
electrokinesis
gel
gene
microbial community
RNA
trophic status
Antarctic Peninsula
Antarctica
Hope Bay
King George Island
Potter Peninsula
South Shetland Islands
West Antarctica
Actinobacteria
Bacteroidetes
Betaproteobacteria
Cyanobacteria
spellingShingle 16S rRNA gene
Bacterioplankton
Denaturing gradient gel electrophoresis
Hope Bay (Antarctic Peninsula)
Maritime Antarctic lakes
Potter Peninsula (King George Island)
bacterium
cluster analysis
comparative study
cyanobacterium
dominance
electrokinesis
gel
gene
microbial community
RNA
trophic status
Antarctic Peninsula
Antarctica
Hope Bay
King George Island
Potter Peninsula
South Shetland Islands
West Antarctica
Actinobacteria
Bacteroidetes
Betaproteobacteria
Cyanobacteria
Schiaffino, M.R.
Unrein, F.
Gasol, J.M.
Farias, M.E.
Estevez, C.
Balagué, V.
Izaguirre, I.
Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status
topic_facet 16S rRNA gene
Bacterioplankton
Denaturing gradient gel electrophoresis
Hope Bay (Antarctic Peninsula)
Maritime Antarctic lakes
Potter Peninsula (King George Island)
bacterium
cluster analysis
comparative study
cyanobacterium
dominance
electrokinesis
gel
gene
microbial community
RNA
trophic status
Antarctic Peninsula
Antarctica
Hope Bay
King George Island
Potter Peninsula
South Shetland Islands
West Antarctica
Actinobacteria
Bacteroidetes
Betaproteobacteria
Cyanobacteria
description The bacterioplankton assemblages of eight maritime Antarctic lakes with a wide range of trophic status and geographic span (six lakes from Hope Bay, Antarctic Peninsula and two from Potter Peninsula, King George Island) were described using denaturing gradient gel electrophoresis and band sequencing during two consecutive austral summers (2003-2004). Analyses of the gels identified a total of 230 bands spread across 57 different positions. Among those bands, 14 were shared between lakes from Hope Bay and Potter Peninsula, 17 were observed only in particular lakes, and 17 were registered both years in the same lake. We successfully reamplified and sequenced 43 bands located in 36 different positions belonging to Bacteroidetes, Actinobacteria, Betaproteobacteria and Cyanobacteria. The closest matches for 63% of the sequenced bands were from Antarctic or from other cold environment clones and sequences already in the databases, suggesting the widespread dominance of microbial communities adapted to cold habitats. The results of the multivariate analyses (Cluster Analysis and CCA) indicated that the nutrient status of the lake influences the bacterioplankton assemblages. © 2009 Springer-Verlag. Fil:Schiaffino, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Unrein, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Izaguirre, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
format Journal/Newspaper
author Schiaffino, M.R.
Unrein, F.
Gasol, J.M.
Farias, M.E.
Estevez, C.
Balagué, V.
Izaguirre, I.
author_facet Schiaffino, M.R.
Unrein, F.
Gasol, J.M.
Farias, M.E.
Estevez, C.
Balagué, V.
Izaguirre, I.
author_sort Schiaffino, M.R.
title Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status
title_short Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status
title_full Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status
title_fullStr Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status
title_full_unstemmed Comparative analysis of bacterioplankton assemblages from maritime Antarctic freshwater lakes with contrasting trophic status
title_sort comparative analysis of bacterioplankton assemblages from maritime antarctic freshwater lakes with contrasting trophic status
url https://hdl.handle.net/20.500.12110/paper_07224060_v32_n6_p923_Schiaffino
genre Antarc*
Antarctic
Antarctic Peninsula
Antarctica
King George Island
South Shetland Islands
West Antarctica
genre_facet Antarc*
Antarctic
Antarctic Peninsula
Antarctica
King George Island
South Shetland Islands
West Antarctica
op_relation http://hdl.handle.net/20.500.12110/paper_07224060_v32_n6_p923_Schiaffino
op_rights info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
op_doi https://doi.org/20.500.12110/paper_07224060_v32_n6_p923_Schiaffino
_version_ 1781053946866958336