Characterization of brain gonadotropin-releasing hormone (GnRH) molecular variants in brain extracts from different perciform fishes from Antarctic waters

• Main Authors: Miranda, L.A., Montaner, A.D., Ansaldo, M., Affanni, J.M., Somoza, G.M.
• Format: Journal/Newspaper
• Language: unknown
• Subjects: GnRH fish; Perciformes; Reproduction; Reverse phase HPLC Radioimmunoassay; Chaenocephalus aceratus; chromatography; gonadorelin; high performance liquid chromatography; Notothenia rossi; protein analysis; Pseudochaenichthys georgianus; radioimmunoassay; Antarc*; Antarctic
• Online Access: https://hdl.handle.net/20.500.12110/paper_07224060_v21_n2_p122_Miranda

Gonadotropin-releasing hormone (GnRH) is the hypothalamic hormone that regulates the reproductive system by stimulating release of gonadotropins from the anterior pituitary gland. The molecular variants of the reproductive neuropeptide GnRH were characterized from brain tissue of three perciform species from Antarctic waters: Pseudochaenichthys georgianus, Chaenocephalus aceratus, and Notothenia rossi. The study involved reverse phase high-performance liquid chromatography (RP-HPLC) followed by radioimmunoassay (RIA) with two antisera that recognize all GnRH variants already identified: PBL 45 and PBL 49. The results showed that brain extracts of P. georgianus, C. aceratus, and N: rossi contain, like those of other perciform fish, three forms of GnRH likely to be: sbGnRH (seabream GnRH), cGnRH-II (chicken GnRH II) and sGnRH (salmon GnRH). They also showed evidence for the presence of a fourth GnRH variant, chromatographically and immunologically different from the other known forms of the vertebrate hormone. Although final conclusions will require isolation, purification, and sequencing of these molecules, these results offer encouraging possibilities of further advances in the characterization of a multiplicity of GnRH molecular variants.