In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content
We investigated the selective predation of mixotrophic and heterotrophic flagellates (MF and HF) on different heterotrophic prokaryote phylotypes (HPP; Bacteria + Archaea) living in natural assemblages from oligotrophic Antarctic lakes. In situ prey preference was analyzed for the first time on diff...
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ftunibueairesbd:todo:paper_01427873_v35_n1_p201_Gerea 2023-10-29T02:31:04+01:00 In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content Gerea, M. Queimaliños, C. Schiaffino, M.R. Izaguirre, I. Forn, I. Massana, R. Unrein, F. https://hdl.handle.net/20.500.12110/paper_01427873_v35_n1_p201_Gerea unknown http://hdl.handle.net/20.500.12110/paper_01427873_v35_n1_p201_Gerea info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar CARD-FISH Heterotrophic flagellates Mixotrophic flagellates Oligotrophic lakes Prey selection catalysis digestive system flagellate heterotrophy lake ecosystem mixotrophy morphology oligotrophic environment phylogenetics phytoplankton predation prokaryote protist Antarctica Actinobacteria Alphaproteobacteria Archaea Bacteria (microorganisms) Bacteroidetes Betaproteobacteria Chrysophyceae Mastigophora (flagellates) Ochromonas Prokaryota Protista Pseudopedinella JOUR ftunibueairesbd https://doi.org/20.500.12110/paper_01427873_v35_n1_p201_Gerea 2023-10-05T01:11:01Z We investigated the selective predation of mixotrophic and heterotrophic flagellates (MF and HF) on different heterotrophic prokaryote phylotypes (HPP; Bacteria + Archaea) living in natural assemblages from oligotrophic Antarctic lakes. In situ prey preference was analyzed for the first time on different mixotrophic taxa (Pseudopedinella sp., Ochromonas-like cells, Chrysophyceae >5 μm). The relative abundances of seven different HPP hybridized by CARD-FISH (catalyzed reporter deposition-fluorescent in situ hybridization) in natural community were compared with the proportions of hybridized cells inside digestive vacuoles. Our results showed some general trends to selectivity over some HPP. Alphaproteobacteria and Betaproteobacteria were the most abundant groups, and strikingly, a negative selection trend was detected in most samples by all bacterivorous protists. In contrast, for Actinobacteria a positive selection trend was observed in most samples, whereas Bacteroidetes seemed to be randomly preyed upon. Interestingly, similar prey preferences were observed in all bacterivorous flagellates. Our results suggest that phylogenetic affiliation determines part of the process of prey selection by protists in these lakes. Nevertheless, other features, such as cell size, morphology and the presence of the S-layer, might also significantly contribute to prey selectivity on the HPP. © 2012 The Author 2012. Published by Oxford University Press. All rights reserved. Fil:Queimaliños, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Schiaffino, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Izaguirre, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Unrein, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Journal/Newspaper Antarc* Antarctic Antarctica Biblioteca Digital FCEN-UBA (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires) |
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Open Polar |
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Biblioteca Digital FCEN-UBA (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires) |
op_collection_id |
ftunibueairesbd |
language |
unknown |
topic |
CARD-FISH Heterotrophic flagellates Mixotrophic flagellates Oligotrophic lakes Prey selection catalysis digestive system flagellate heterotrophy lake ecosystem mixotrophy morphology oligotrophic environment phylogenetics phytoplankton predation prokaryote protist Antarctica Actinobacteria Alphaproteobacteria Archaea Bacteria (microorganisms) Bacteroidetes Betaproteobacteria Chrysophyceae Mastigophora (flagellates) Ochromonas Prokaryota Protista Pseudopedinella |
spellingShingle |
CARD-FISH Heterotrophic flagellates Mixotrophic flagellates Oligotrophic lakes Prey selection catalysis digestive system flagellate heterotrophy lake ecosystem mixotrophy morphology oligotrophic environment phylogenetics phytoplankton predation prokaryote protist Antarctica Actinobacteria Alphaproteobacteria Archaea Bacteria (microorganisms) Bacteroidetes Betaproteobacteria Chrysophyceae Mastigophora (flagellates) Ochromonas Prokaryota Protista Pseudopedinella Gerea, M. Queimaliños, C. Schiaffino, M.R. Izaguirre, I. Forn, I. Massana, R. Unrein, F. In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content |
topic_facet |
CARD-FISH Heterotrophic flagellates Mixotrophic flagellates Oligotrophic lakes Prey selection catalysis digestive system flagellate heterotrophy lake ecosystem mixotrophy morphology oligotrophic environment phylogenetics phytoplankton predation prokaryote protist Antarctica Actinobacteria Alphaproteobacteria Archaea Bacteria (microorganisms) Bacteroidetes Betaproteobacteria Chrysophyceae Mastigophora (flagellates) Ochromonas Prokaryota Protista Pseudopedinella |
description |
We investigated the selective predation of mixotrophic and heterotrophic flagellates (MF and HF) on different heterotrophic prokaryote phylotypes (HPP; Bacteria + Archaea) living in natural assemblages from oligotrophic Antarctic lakes. In situ prey preference was analyzed for the first time on different mixotrophic taxa (Pseudopedinella sp., Ochromonas-like cells, Chrysophyceae >5 μm). The relative abundances of seven different HPP hybridized by CARD-FISH (catalyzed reporter deposition-fluorescent in situ hybridization) in natural community were compared with the proportions of hybridized cells inside digestive vacuoles. Our results showed some general trends to selectivity over some HPP. Alphaproteobacteria and Betaproteobacteria were the most abundant groups, and strikingly, a negative selection trend was detected in most samples by all bacterivorous protists. In contrast, for Actinobacteria a positive selection trend was observed in most samples, whereas Bacteroidetes seemed to be randomly preyed upon. Interestingly, similar prey preferences were observed in all bacterivorous flagellates. Our results suggest that phylogenetic affiliation determines part of the process of prey selection by protists in these lakes. Nevertheless, other features, such as cell size, morphology and the presence of the S-layer, might also significantly contribute to prey selectivity on the HPP. © 2012 The Author 2012. Published by Oxford University Press. All rights reserved. Fil:Queimaliños, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Schiaffino, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Izaguirre, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Unrein, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
format |
Journal/Newspaper |
author |
Gerea, M. Queimaliños, C. Schiaffino, M.R. Izaguirre, I. Forn, I. Massana, R. Unrein, F. |
author_facet |
Gerea, M. Queimaliños, C. Schiaffino, M.R. Izaguirre, I. Forn, I. Massana, R. Unrein, F. |
author_sort |
Gerea, M. |
title |
In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content |
title_short |
In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content |
title_full |
In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content |
title_fullStr |
In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content |
title_full_unstemmed |
In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content |
title_sort |
in situ prey selection of mixotrophic and heterotrophic flagellates in antarctic oligotrophic lakes: an analysis of the digestive vacuole content |
url |
https://hdl.handle.net/20.500.12110/paper_01427873_v35_n1_p201_Gerea |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
http://hdl.handle.net/20.500.12110/paper_01427873_v35_n1_p201_Gerea |
op_rights |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
op_doi |
https://doi.org/20.500.12110/paper_01427873_v35_n1_p201_Gerea |
_version_ |
1781065496700911616 |