An efficient enzymatic preparation of 20-pregnane succinates: chemoenzymatic synthesis of 20β-hemisuccinyloxy-5αH-pregnan-3-one

• Main Authors: Monsalve, L.N., Machado Rada, M.Y., Ghini, A.A., Baldessari, A.
• Format: Journal/Newspaper
• Language: unknown
• Subjects: 20-Pregnane succinates; Candida antarctica lipase B; Enzymatic stereoselective transesterification; 20 pregnane succinate derivative; 20beta hemisuccinyloxy 5alphah pregnan 3 one; hydroxyl group; pregnane derivative; steroid; succinic acid derivative; triacylglycerol lipase; unclassified drug; article; catalysis; chemical structure; enzyme isolation; priority journal; synthesis; transesterification; Candida antarctica; Antarc*; Antarctica
• Online Access: https://hdl.handle.net/20.500.12110/paper_00404020_v64_n8_p1721_Monsalve

Lipase-catalyzed transesterification of the 20 hydroxyl group in a series of pregnanes afforded novel 20-ethyl succinates that are not possible to prepare following the traditional synthetic methods. The reaction is stereoselective. The enzyme reacts selectively with the 20β epimers therefore only the 20β-succinyloxy derivatives are obtained. These compounds are obtained in variable yield, depending on the substitution in the ring A. The enzymatic approach allowed, for the first time, the synthesis of 20β-hemisuccinyloxy-5αH-pregnan-3-one, novel compound useful as a precursor of steroid-protein conjugates. © 2007 Elsevier Ltd. All rights reserved. Fil:Monsalve, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ghini, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Baldessari, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.