Sulfated polysaccharides from the red seaweed Georgiella confluens
The water-soluble polysaccharides from Georgiella confluens, collected in Antarctica, were fractionated with cetrimide. The complexed material was subjected to fractional solubilization in solutions of increasing sodium chloride concentration. The initially extracted polysaccharide and the major fra...
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ftunibueairesbd:todo:paper_00086215_v337_n1_p57_Kolender 2023-10-29T02:32:32+01:00 Sulfated polysaccharides from the red seaweed Georgiella confluens Kolender, A.A. Matulewicz, M.C. https://hdl.handle.net/20.500.12110/paper_00086215_v337_n1_p57_Kolender unknown http://hdl.handle.net/20.500.12110/paper_00086215_v337_n1_p57_Kolender info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Agarans Galactans sulfated Polysaccharides red algal Structural analysis Gas chromatography Nuclear magnetic resonance spectroscopy Sodium chloride Solubility Solutions Xylose Sulfated polysaccharides 2 o methyl levo galactose 3,6 anhydro 2 o methyl levo galactose 6 o methyl dextro galactose carbohydrate derivative carbon 13 cetrimide galactose monosaccharide polysaccharide sulfate sulfur derivative unclassified drug water amination Antarctica article carbohydrate analysis chemical reaction concentration response conformation controlled study degradation diastereoisomer ethylation fractionation methylation nonhuman priority journal seaweed JOUR ftunibueairesbd https://doi.org/20.500.12110/paper_00086215_v337_n1_p57_Kolender 2023-10-05T01:54:27Z The water-soluble polysaccharides from Georgiella confluens, collected in Antarctica, were fractionated with cetrimide. The complexed material was subjected to fractional solubilization in solutions of increasing sodium chloride concentration. The initially extracted polysaccharide and the major fraction isolated, soluble in 0.5 M NaCl, were studied. These are sulfated xylogalactans naturally rich in methylated sugar residues, comprising of 3,6-anhydro-2-O-methyl-L-galactose, 2-O-methyl-L-galactose and 6-O-methyl-D-galactose. Structural analysis was carried out by methylation, ethylation, desulfation-ethylation, desulfation-methylation, Smith degradation, 13C NMR spectroscopy and determination of the absolute configuration of monosaccharides by gas chromatography of diastereomeric derivatives produced by reductive amination. The results indicated the presence of an agaran backbone with an unusual substitution pattern: sulfation mainly at the 3-position of the α-L-galactose units and the presence of xylose side chains at the 4-position of the β-D-galactose residues. © 2002 Elsevier Science Ltd. All rights reserved. Journal/Newspaper Antarc* Antarctica Biblioteca Digital FCEN-UBA (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires) |
institution |
Open Polar |
collection |
Biblioteca Digital FCEN-UBA (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires) |
op_collection_id |
ftunibueairesbd |
language |
unknown |
topic |
Agarans Galactans sulfated Polysaccharides red algal Structural analysis Gas chromatography Nuclear magnetic resonance spectroscopy Sodium chloride Solubility Solutions Xylose Sulfated polysaccharides 2 o methyl levo galactose 3,6 anhydro 2 o methyl levo galactose 6 o methyl dextro galactose carbohydrate derivative carbon 13 cetrimide galactose monosaccharide polysaccharide sulfate sulfur derivative unclassified drug water amination Antarctica article carbohydrate analysis chemical reaction concentration response conformation controlled study degradation diastereoisomer ethylation fractionation methylation nonhuman priority journal seaweed |
spellingShingle |
Agarans Galactans sulfated Polysaccharides red algal Structural analysis Gas chromatography Nuclear magnetic resonance spectroscopy Sodium chloride Solubility Solutions Xylose Sulfated polysaccharides 2 o methyl levo galactose 3,6 anhydro 2 o methyl levo galactose 6 o methyl dextro galactose carbohydrate derivative carbon 13 cetrimide galactose monosaccharide polysaccharide sulfate sulfur derivative unclassified drug water amination Antarctica article carbohydrate analysis chemical reaction concentration response conformation controlled study degradation diastereoisomer ethylation fractionation methylation nonhuman priority journal seaweed Kolender, A.A. Matulewicz, M.C. Sulfated polysaccharides from the red seaweed Georgiella confluens |
topic_facet |
Agarans Galactans sulfated Polysaccharides red algal Structural analysis Gas chromatography Nuclear magnetic resonance spectroscopy Sodium chloride Solubility Solutions Xylose Sulfated polysaccharides 2 o methyl levo galactose 3,6 anhydro 2 o methyl levo galactose 6 o methyl dextro galactose carbohydrate derivative carbon 13 cetrimide galactose monosaccharide polysaccharide sulfate sulfur derivative unclassified drug water amination Antarctica article carbohydrate analysis chemical reaction concentration response conformation controlled study degradation diastereoisomer ethylation fractionation methylation nonhuman priority journal seaweed |
description |
The water-soluble polysaccharides from Georgiella confluens, collected in Antarctica, were fractionated with cetrimide. The complexed material was subjected to fractional solubilization in solutions of increasing sodium chloride concentration. The initially extracted polysaccharide and the major fraction isolated, soluble in 0.5 M NaCl, were studied. These are sulfated xylogalactans naturally rich in methylated sugar residues, comprising of 3,6-anhydro-2-O-methyl-L-galactose, 2-O-methyl-L-galactose and 6-O-methyl-D-galactose. Structural analysis was carried out by methylation, ethylation, desulfation-ethylation, desulfation-methylation, Smith degradation, 13C NMR spectroscopy and determination of the absolute configuration of monosaccharides by gas chromatography of diastereomeric derivatives produced by reductive amination. The results indicated the presence of an agaran backbone with an unusual substitution pattern: sulfation mainly at the 3-position of the α-L-galactose units and the presence of xylose side chains at the 4-position of the β-D-galactose residues. © 2002 Elsevier Science Ltd. All rights reserved. |
format |
Journal/Newspaper |
author |
Kolender, A.A. Matulewicz, M.C. |
author_facet |
Kolender, A.A. Matulewicz, M.C. |
author_sort |
Kolender, A.A. |
title |
Sulfated polysaccharides from the red seaweed Georgiella confluens |
title_short |
Sulfated polysaccharides from the red seaweed Georgiella confluens |
title_full |
Sulfated polysaccharides from the red seaweed Georgiella confluens |
title_fullStr |
Sulfated polysaccharides from the red seaweed Georgiella confluens |
title_full_unstemmed |
Sulfated polysaccharides from the red seaweed Georgiella confluens |
title_sort |
sulfated polysaccharides from the red seaweed georgiella confluens |
url |
https://hdl.handle.net/20.500.12110/paper_00086215_v337_n1_p57_Kolender |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://hdl.handle.net/20.500.12110/paper_00086215_v337_n1_p57_Kolender |
op_rights |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
op_doi |
https://doi.org/20.500.12110/paper_00086215_v337_n1_p57_Kolender |
_version_ |
1781054146412019712 |