Pseudomonas extremaustralis sp. nov., a poly(3-hydroxybutyrate) producer isolated from an antarctic environment

• Main Authors: Pettinari, María Julia, Tribelli, Paula María, Méndez, Beatriz Silvia
• Language: unknown
• Published: 2009
• Subjects: arginine deiminase; beta galactosidase; casein; esculin; gelatin; indole; poly(3 hydroxybutyric acid); RNA 16S; urease; acidification; Antarctica; article; bacterial growth; bacterial strain; controlled study; denitrification; DNA DNA hybridization; gene sequence; hydrolysis; nitrate reducing bacterium; nonhuman; nucleotide sequence; priority journal; Pseudomonas; pseudomonas extremaustralis; Pseudomonas sensu stricto; temperature sensitivity; Antarctic Regions; DNA; Bacterial; Ribosomal; Hydroxybutyrates; Molecular Sequence Data; Phylogeny; Polyesters; RNA; 16S; Bacteria (microorganisms); Negibacteria; Pseudomonas veronii; Antarctic; Argentina; Antarc*
• Online Access: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v59_n5_p514_Lopez; https://hdl.handle.net/20.500.12110/paper_03438651_v59_n5_p514_Lopez

A Gram-negative, mobile, rod-shaped, non-spore-forming bacterium (strain 14-3T) was isolated from a temporary pond in Antarctica. On the basis of 16S rRNA gene sequence similarity, strain 14-3T was shown to belong to the genus Pseudomonas sensu stricto. Physiological and biochemical tests supported the phylogenetic affiliation. Strain 14-3T is closely related to Pseudomonas veronii DSM 11331T, sharing 99.7% sequence similarity. DNA-DNA hybridization experiments between the two strains showed only moderate reassociation similarity (35.1%). Tests for arginine dihydrolase and nitrate reduction were positive, while those for denitrification, indol production, glucose acidification, urease, -galactosidase, esculin, caseine and gelatin hydrolysis were negative. Growth of this bacterium occurred in a range from 4 to 37°C but not at 42°C. It accumulated poly(3-hydroxybutyrate) when grown on sodium octanoate medium. Strain 14-3T therefore represents the type strain of a new species, for which the name Pseudomonas extremaustralis sp. nov. is proposed. The type strain 14-3T has been deposited as DSM 17835T and as CIP 109839T. © 2009 Springer Science+Business Media, LLC. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tribelli, P.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.