Sulfated polysaccharides from the red seaweed Georgiella confluens

• Language: unknown
• Published: 2002
• Subjects: Agarans; Galactans; sulfated; Polysaccharides; red algal; Structural analysis; Gas chromatography; Nuclear magnetic resonance spectroscopy; Sodium chloride; Solubility; Solutions; Xylose; Sulfated polysaccharides; 2 o methyl levo galactose; 3,6 anhydro 2 o methyl levo galactose; 6 o methyl dextro galactose; carbohydrate derivative; carbon 13; cetrimide; galactose; monosaccharide; polysaccharide sulfate; sulfur derivative; unclassified drug; water; amination; Antarctica; article; carbohydrate analysis; chemical reaction; concentration response; conformation; controlled study; degradation; diastereoisomer; ethylation; fractionation; methylation; nonhuman; priority journal; seaweed; Antarc*
• Online Access: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v337_n1_p57_Kolender; https://hdl.handle.net/20.500.12110/paper_00086215_v337_n1_p57_Kolender

The water-soluble polysaccharides from Georgiella confluens, collected in Antarctica, were fractionated with cetrimide. The complexed material was subjected to fractional solubilization in solutions of increasing sodium chloride concentration. The initially extracted polysaccharide and the major fraction isolated, soluble in 0.5 M NaCl, were studied. These are sulfated xylogalactans naturally rich in methylated sugar residues, comprising of 3,6-anhydro-2-O-methyl-L-galactose, 2-O-methyl-L-galactose and 6-O-methyl-D-galactose. Structural analysis was carried out by methylation, ethylation, desulfation-ethylation, desulfation-methylation, Smith degradation, 13C NMR spectroscopy and determination of the absolute configuration of monosaccharides by gas chromatography of diastereomeric derivatives produced by reductive amination. The results indicated the presence of an agaran backbone with an unusual substitution pattern: sulfation mainly at the 3-position of the α-L-galactose units and the presence of xylose side chains at the 4-position of the β-D-galactose residues. © 2002 Elsevier Science Ltd. All rights reserved.