Exploring bacterial community composition and immune gene expression of European eel larvae (Anguilla anguilla) in relation to first-feeding diets
: European eel (Anguilla anguilla) is a commercially important species for fisheries and aquaculture in Europe and the attempt to close the lifecycle in captivity is still at pioneering stage. The first feeding stage of this species is characterized by a critical period between 20 to 24 days post ha...
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Online Access: | https://hdl.handle.net/11585/942974 https://doi.org/10.1371/journal.pone.0288734 |
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ftunibolognairis:oai:cris.unibo.it:11585/942974 2024-04-14T08:00:59+00:00 Exploring bacterial community composition and immune gene expression of European eel larvae (Anguilla anguilla) in relation to first-feeding diets Bandara, Kasun A Benini, Elisa Politis, Sebastian N Conceição, Luis E C Santos, André Sørensen, Sune Riis Tomkiewicz, Jonna Vadstein, Olav Bandara, Kasun A Benini, Elisa Politis, Sebastian N Conceição, Luis E C Santos, André Sørensen, Sune Rii Tomkiewicz, Jonna Vadstein, Olav 2023 STAMPA https://hdl.handle.net/11585/942974 https://doi.org/10.1371/journal.pone.0288734 eng eng info:eu-repo/semantics/altIdentifier/pmid/37498931 info:eu-repo/semantics/altIdentifier/wos/WOS:001066222400054 volume:18 issue:7 firstpage:1 lastpage:24 numberofpages:24 journal:PLOS ONE https://hdl.handle.net/11585/942974 doi:10.1371/journal.pone.0288734 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85165917883 info:eu-repo/semantics/openAccess amplicon Anguilla anguilla Article diet feeding female gene expression regulation gene sequence genetic variability host bacterium interaction hsp90 gene immune response immunocompetence larval stage male microbial community mortality rate nonhuman physiological stre survival rate upregulation animal aquaculture gene expression genetic larva veterinary medicine info:eu-repo/semantics/article 2023 ftunibolognairis https://doi.org/10.1371/journal.pone.0288734 2024-03-21T16:59:58Z : European eel (Anguilla anguilla) is a commercially important species for fisheries and aquaculture in Europe and the attempt to close the lifecycle in captivity is still at pioneering stage. The first feeding stage of this species is characterized by a critical period between 20 to 24 days post hatch (dph), which is associated with mortalities, indicating the point of no return. We hypothesized that this critical period might also be associated with larvae-bacterial interactions and the larval immune status. To test this, bacterial community composition and expression of immune and stress-related genes of hatchery-produced larvae were explored from the end of endogenous feeding (9 dph) until 28 dph, in response to three experimental first-feeding diets (Diet 1, Diet 2 and Diet 3). Changes in the water bacterial community composition were also followed. Results revealed that the larval stress/repair mechanism was activated during this critical period, marked by an upregulated expression of the hsp90 gene, independent of the diet fed. At the same time, a shift towards a potentially detrimental larval bacterial community was observed in all dietary groups. Here, a significant reduction in evenness of the larval bacterial community was observed, and several amplicon sequence variants belonging to potentially harmful bacterial genera were more abundant. This indicates that detrimental larvae-bacteria interactions were likely involved in the mortality observed. Beyond the critical period, the highest survival was registered for larvae fed Diet 3. Interestingly, genes encoding for pathogen recognition receptor TLR18 and complement component C1QC were upregulated in this group, potentially indicating a higher immunocompetency that facilitated a more successful handling of the harmful bacteria that dominated the bacterial community of larvae on 22 dph, ultimately leading to better survival, compared to the other two groups. Article in Journal/Newspaper Anguilla anguilla European eel IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) PLOS ONE 18 7 e0288734 |
institution |
Open Polar |
collection |
IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
op_collection_id |
ftunibolognairis |
language |
English |
topic |
amplicon Anguilla anguilla Article diet feeding female gene expression regulation gene sequence genetic variability host bacterium interaction hsp90 gene immune response immunocompetence larval stage male microbial community mortality rate nonhuman physiological stre survival rate upregulation animal aquaculture gene expression genetic larva veterinary medicine |
spellingShingle |
amplicon Anguilla anguilla Article diet feeding female gene expression regulation gene sequence genetic variability host bacterium interaction hsp90 gene immune response immunocompetence larval stage male microbial community mortality rate nonhuman physiological stre survival rate upregulation animal aquaculture gene expression genetic larva veterinary medicine Bandara, Kasun A Benini, Elisa Politis, Sebastian N Conceição, Luis E C Santos, André Sørensen, Sune Riis Tomkiewicz, Jonna Vadstein, Olav Exploring bacterial community composition and immune gene expression of European eel larvae (Anguilla anguilla) in relation to first-feeding diets |
topic_facet |
amplicon Anguilla anguilla Article diet feeding female gene expression regulation gene sequence genetic variability host bacterium interaction hsp90 gene immune response immunocompetence larval stage male microbial community mortality rate nonhuman physiological stre survival rate upregulation animal aquaculture gene expression genetic larva veterinary medicine |
description |
: European eel (Anguilla anguilla) is a commercially important species for fisheries and aquaculture in Europe and the attempt to close the lifecycle in captivity is still at pioneering stage. The first feeding stage of this species is characterized by a critical period between 20 to 24 days post hatch (dph), which is associated with mortalities, indicating the point of no return. We hypothesized that this critical period might also be associated with larvae-bacterial interactions and the larval immune status. To test this, bacterial community composition and expression of immune and stress-related genes of hatchery-produced larvae were explored from the end of endogenous feeding (9 dph) until 28 dph, in response to three experimental first-feeding diets (Diet 1, Diet 2 and Diet 3). Changes in the water bacterial community composition were also followed. Results revealed that the larval stress/repair mechanism was activated during this critical period, marked by an upregulated expression of the hsp90 gene, independent of the diet fed. At the same time, a shift towards a potentially detrimental larval bacterial community was observed in all dietary groups. Here, a significant reduction in evenness of the larval bacterial community was observed, and several amplicon sequence variants belonging to potentially harmful bacterial genera were more abundant. This indicates that detrimental larvae-bacteria interactions were likely involved in the mortality observed. Beyond the critical period, the highest survival was registered for larvae fed Diet 3. Interestingly, genes encoding for pathogen recognition receptor TLR18 and complement component C1QC were upregulated in this group, potentially indicating a higher immunocompetency that facilitated a more successful handling of the harmful bacteria that dominated the bacterial community of larvae on 22 dph, ultimately leading to better survival, compared to the other two groups. |
author2 |
Bandara, Kasun A Benini, Elisa Politis, Sebastian N Conceição, Luis E C Santos, André Sørensen, Sune Rii Tomkiewicz, Jonna Vadstein, Olav |
format |
Article in Journal/Newspaper |
author |
Bandara, Kasun A Benini, Elisa Politis, Sebastian N Conceição, Luis E C Santos, André Sørensen, Sune Riis Tomkiewicz, Jonna Vadstein, Olav |
author_facet |
Bandara, Kasun A Benini, Elisa Politis, Sebastian N Conceição, Luis E C Santos, André Sørensen, Sune Riis Tomkiewicz, Jonna Vadstein, Olav |
author_sort |
Bandara, Kasun A |
title |
Exploring bacterial community composition and immune gene expression of European eel larvae (Anguilla anguilla) in relation to first-feeding diets |
title_short |
Exploring bacterial community composition and immune gene expression of European eel larvae (Anguilla anguilla) in relation to first-feeding diets |
title_full |
Exploring bacterial community composition and immune gene expression of European eel larvae (Anguilla anguilla) in relation to first-feeding diets |
title_fullStr |
Exploring bacterial community composition and immune gene expression of European eel larvae (Anguilla anguilla) in relation to first-feeding diets |
title_full_unstemmed |
Exploring bacterial community composition and immune gene expression of European eel larvae (Anguilla anguilla) in relation to first-feeding diets |
title_sort |
exploring bacterial community composition and immune gene expression of european eel larvae (anguilla anguilla) in relation to first-feeding diets |
publishDate |
2023 |
url |
https://hdl.handle.net/11585/942974 https://doi.org/10.1371/journal.pone.0288734 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/37498931 info:eu-repo/semantics/altIdentifier/wos/WOS:001066222400054 volume:18 issue:7 firstpage:1 lastpage:24 numberofpages:24 journal:PLOS ONE https://hdl.handle.net/11585/942974 doi:10.1371/journal.pone.0288734 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85165917883 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0288734 |
container_title |
PLOS ONE |
container_volume |
18 |
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container_start_page |
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