Genetic subdivision and candidate genes under selection in North American grey wolves
Previous genetic studies of the highly mobile grey wolf (Canis lupus) found population structure that coincides with habitat and phenotype differences. We hypothesized that these ecologically distinct populations (ecotypes) should exhibit signatures of selection in genes related to morphology, coat...
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ftunibolognairis:oai:cris.unibo.it:11585/902893 2024-09-15T18:01:22+00:00 Genetic subdivision and candidate genes under selection in North American grey wolves Schweizer R. M. VonHoldt B. M. Harrigan R. Knowles J. C. Musiani M. Coltman D. Novembre J. Wayne R. K. Schweizer R.M. VonHoldt B.M. Harrigan R. Knowles J.C. Musiani M. Coltman D. Novembre J. Wayne R.K. 2016 ELETTRONICO https://hdl.handle.net/11585/902893 https://doi.org/10.1111/mec.13364 eng eng info:eu-repo/semantics/altIdentifier/pmid/26333947 info:eu-repo/semantics/altIdentifier/wos/WOS:000367908800025 volume:25 issue:1 firstpage:380 lastpage:402 numberofpages:23 journal:MOLECULAR ECOLOGY https://hdl.handle.net/11585/902893 doi:10.1111/mec.13364 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84954074556 adaptation Canis lupu ecological genetic genomic mammal population genetic SNP array info:eu-repo/semantics/article 2016 ftunibolognairis https://doi.org/10.1111/mec.13364 2024-07-01T14:08:29Z Previous genetic studies of the highly mobile grey wolf (Canis lupus) found population structure that coincides with habitat and phenotype differences. We hypothesized that these ecologically distinct populations (ecotypes) should exhibit signatures of selection in genes related to morphology, coat colour and metabolism. To test these predictions, we quantified population structure related to habitat using a genotyping array to assess variation in 42 036 single-nucleotide polymorphisms (SNPs) in 111 North American grey wolves. Using these SNP data and individual-level measurements of 12 environmental variables, we identified six ecotypes: West Forest, Boreal Forest, Arctic, High Arctic, British Columbia and Atlantic Forest. Next, we explored signals of selection across these wolf ecotypes through the use of three complementary methods to detect selection: FST/haplotype homozygosity bivariate percentilae, bayescan, and environmentally correlated directional selection with bayenv. Across all methods, we found consistent signals of selection on genes related to morphology, coat coloration, metabolism, as predicted, as well as vision and hearing. In several high-ranking candidate genes, including LEPR, TYR and SLC14A2, we found variation in allele frequencies that follow environmental changes in temperature and precipitation, a result that is consistent with local adaptation rather than genetic drift. Our findings show that local adaptation can occur despite gene flow in a highly mobile species and can be detected through a moderately dense genomic scan. These patterns of local adaptation revealed by SNP genotyping likely reflect high fidelity to natal habitats of dispersing wolves, strong ecological divergence among habitats, and moderate levels of linkage in the wolf genome. Article in Journal/Newspaper Canis lupus IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) Molecular Ecology 25 1 380 402 |
institution |
Open Polar |
collection |
IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
op_collection_id |
ftunibolognairis |
language |
English |
topic |
adaptation Canis lupu ecological genetic genomic mammal population genetic SNP array |
spellingShingle |
adaptation Canis lupu ecological genetic genomic mammal population genetic SNP array Schweizer R. M. VonHoldt B. M. Harrigan R. Knowles J. C. Musiani M. Coltman D. Novembre J. Wayne R. K. Genetic subdivision and candidate genes under selection in North American grey wolves |
topic_facet |
adaptation Canis lupu ecological genetic genomic mammal population genetic SNP array |
description |
Previous genetic studies of the highly mobile grey wolf (Canis lupus) found population structure that coincides with habitat and phenotype differences. We hypothesized that these ecologically distinct populations (ecotypes) should exhibit signatures of selection in genes related to morphology, coat colour and metabolism. To test these predictions, we quantified population structure related to habitat using a genotyping array to assess variation in 42 036 single-nucleotide polymorphisms (SNPs) in 111 North American grey wolves. Using these SNP data and individual-level measurements of 12 environmental variables, we identified six ecotypes: West Forest, Boreal Forest, Arctic, High Arctic, British Columbia and Atlantic Forest. Next, we explored signals of selection across these wolf ecotypes through the use of three complementary methods to detect selection: FST/haplotype homozygosity bivariate percentilae, bayescan, and environmentally correlated directional selection with bayenv. Across all methods, we found consistent signals of selection on genes related to morphology, coat coloration, metabolism, as predicted, as well as vision and hearing. In several high-ranking candidate genes, including LEPR, TYR and SLC14A2, we found variation in allele frequencies that follow environmental changes in temperature and precipitation, a result that is consistent with local adaptation rather than genetic drift. Our findings show that local adaptation can occur despite gene flow in a highly mobile species and can be detected through a moderately dense genomic scan. These patterns of local adaptation revealed by SNP genotyping likely reflect high fidelity to natal habitats of dispersing wolves, strong ecological divergence among habitats, and moderate levels of linkage in the wolf genome. |
author2 |
Schweizer R.M. VonHoldt B.M. Harrigan R. Knowles J.C. Musiani M. Coltman D. Novembre J. Wayne R.K. |
format |
Article in Journal/Newspaper |
author |
Schweizer R. M. VonHoldt B. M. Harrigan R. Knowles J. C. Musiani M. Coltman D. Novembre J. Wayne R. K. |
author_facet |
Schweizer R. M. VonHoldt B. M. Harrigan R. Knowles J. C. Musiani M. Coltman D. Novembre J. Wayne R. K. |
author_sort |
Schweizer R. M. |
title |
Genetic subdivision and candidate genes under selection in North American grey wolves |
title_short |
Genetic subdivision and candidate genes under selection in North American grey wolves |
title_full |
Genetic subdivision and candidate genes under selection in North American grey wolves |
title_fullStr |
Genetic subdivision and candidate genes under selection in North American grey wolves |
title_full_unstemmed |
Genetic subdivision and candidate genes under selection in North American grey wolves |
title_sort |
genetic subdivision and candidate genes under selection in north american grey wolves |
publishDate |
2016 |
url |
https://hdl.handle.net/11585/902893 https://doi.org/10.1111/mec.13364 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/26333947 info:eu-repo/semantics/altIdentifier/wos/WOS:000367908800025 volume:25 issue:1 firstpage:380 lastpage:402 numberofpages:23 journal:MOLECULAR ECOLOGY https://hdl.handle.net/11585/902893 doi:10.1111/mec.13364 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84954074556 |
op_doi |
https://doi.org/10.1111/mec.13364 |
container_title |
Molecular Ecology |
container_volume |
25 |
container_issue |
1 |
container_start_page |
380 |
op_container_end_page |
402 |
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1810438514611847168 |