Unravelling the changes during induced vitellogenesis in female European eel through RNA-Seq: What happens to the liver?
The life cycle of European eel (Anguilla anguilla), a catadromous species, is complex and enigmatic. In nature, during the silvering process prior to their long spawning migration, reproductive development is arrested, and they cease feeding. In studies of reproduction using hormonal induction, eels...
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ftunibolognairis:oai:cris.unibo.it:11585/900938 2024-04-14T08:01:03+00:00 Unravelling the changes during induced vitellogenesis in female European eel through RNA-Seq: What happens to the liver? Bertolini, Francesca Jørgensen, Michelle Grace Pinto Henkel, Christiaan Dufour, Sylvie Tomkiewicz, Jonna Bertolini, Francesca Jørgensen, Michelle Grace Pinto Henkel, Christiaan Dufour, Sylvie Tomkiewicz, Jonna 2020 ELETTRONICO https://hdl.handle.net/11585/900938 https://doi.org/10.1371/journal.pone.0236438 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0236438 eng eng info:eu-repo/semantics/altIdentifier/pmid/32790680 info:eu-repo/semantics/altIdentifier/wos/WOS:000562668300029 volume:15 issue:8 firstpage:1 lastpage:19 numberofpages:19 journal:PLOS ONE https://hdl.handle.net/11585/900938 doi:10.1371/journal.pone.0236438 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85089537670 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0236438 info:eu-repo/semantics/openAccess Anguilla Animal Female Liver RNA-Seq Reproduction Transcriptome Vitellogenesis info:eu-repo/semantics/article 2020 ftunibolognairis https://doi.org/10.1371/journal.pone.0236438 2024-03-21T17:08:10Z The life cycle of European eel (Anguilla anguilla), a catadromous species, is complex and enigmatic. In nature, during the silvering process prior to their long spawning migration, reproductive development is arrested, and they cease feeding. In studies of reproduction using hormonal induction, eels are equivalently not feed. Therefore, in female eels that undergo vitellogenesis, the liver plays different, essential roles being involved both in vitellogenins synthesis and in reallocating resources for the maintenance of vital functions, performing the transoceanic reproductive migration and completing reproductive development. The present work aimed at unravelling the major transcriptomic changes that occur in the liver during induced vitellogenesis in female eels. mRNA-Seq data from 16 animals (eight before induced vitellogenesis and eight after nine weeks of hormonal treatment) were generated and differential expression analysis was performed comparing the two groups. This analysis detected 1,328 upregulated and 1,490 downregulated transcripts. Overrepresentation analysis of the upregulated genes included biological processes related to biosynthesis, response to estrogens, mitochondrial activity and localization, while downregulated genes were enriched in processes related to morphogenesis and development of several organs and tissues, including liver and immune system. Among key genes, the upregulated ones included vitellogenin genes (VTG1andVTG2) that are central in vitellogenesis, together withESR1and two novel genes not previously investigated in European eel (LMAN1andNUPR1), which have been linked with reproduction in other species. Moreover, several upregulated genes, such asCYC1,ELOVL5,KARSandACSS1, are involved in the management of the effect of fasting andNOTCH,VEGFAandNCORare linked with development, autophagy and liver maintenance in other species. These results increase the understanding of the molecular changes that occur in the liver during vitellogenesis in this complex and distinctive fish ... Article in Journal/Newspaper Anguilla anguilla European eel IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) PLOS ONE 15 8 e0236438 |
institution |
Open Polar |
collection |
IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
op_collection_id |
ftunibolognairis |
language |
English |
topic |
Anguilla Animal Female Liver RNA-Seq Reproduction Transcriptome Vitellogenesis |
spellingShingle |
Anguilla Animal Female Liver RNA-Seq Reproduction Transcriptome Vitellogenesis Bertolini, Francesca Jørgensen, Michelle Grace Pinto Henkel, Christiaan Dufour, Sylvie Tomkiewicz, Jonna Unravelling the changes during induced vitellogenesis in female European eel through RNA-Seq: What happens to the liver? |
topic_facet |
Anguilla Animal Female Liver RNA-Seq Reproduction Transcriptome Vitellogenesis |
description |
The life cycle of European eel (Anguilla anguilla), a catadromous species, is complex and enigmatic. In nature, during the silvering process prior to their long spawning migration, reproductive development is arrested, and they cease feeding. In studies of reproduction using hormonal induction, eels are equivalently not feed. Therefore, in female eels that undergo vitellogenesis, the liver plays different, essential roles being involved both in vitellogenins synthesis and in reallocating resources for the maintenance of vital functions, performing the transoceanic reproductive migration and completing reproductive development. The present work aimed at unravelling the major transcriptomic changes that occur in the liver during induced vitellogenesis in female eels. mRNA-Seq data from 16 animals (eight before induced vitellogenesis and eight after nine weeks of hormonal treatment) were generated and differential expression analysis was performed comparing the two groups. This analysis detected 1,328 upregulated and 1,490 downregulated transcripts. Overrepresentation analysis of the upregulated genes included biological processes related to biosynthesis, response to estrogens, mitochondrial activity and localization, while downregulated genes were enriched in processes related to morphogenesis and development of several organs and tissues, including liver and immune system. Among key genes, the upregulated ones included vitellogenin genes (VTG1andVTG2) that are central in vitellogenesis, together withESR1and two novel genes not previously investigated in European eel (LMAN1andNUPR1), which have been linked with reproduction in other species. Moreover, several upregulated genes, such asCYC1,ELOVL5,KARSandACSS1, are involved in the management of the effect of fasting andNOTCH,VEGFAandNCORare linked with development, autophagy and liver maintenance in other species. These results increase the understanding of the molecular changes that occur in the liver during vitellogenesis in this complex and distinctive fish ... |
author2 |
Bertolini, Francesca Jørgensen, Michelle Grace Pinto Henkel, Christiaan Dufour, Sylvie Tomkiewicz, Jonna |
format |
Article in Journal/Newspaper |
author |
Bertolini, Francesca Jørgensen, Michelle Grace Pinto Henkel, Christiaan Dufour, Sylvie Tomkiewicz, Jonna |
author_facet |
Bertolini, Francesca Jørgensen, Michelle Grace Pinto Henkel, Christiaan Dufour, Sylvie Tomkiewicz, Jonna |
author_sort |
Bertolini, Francesca |
title |
Unravelling the changes during induced vitellogenesis in female European eel through RNA-Seq: What happens to the liver? |
title_short |
Unravelling the changes during induced vitellogenesis in female European eel through RNA-Seq: What happens to the liver? |
title_full |
Unravelling the changes during induced vitellogenesis in female European eel through RNA-Seq: What happens to the liver? |
title_fullStr |
Unravelling the changes during induced vitellogenesis in female European eel through RNA-Seq: What happens to the liver? |
title_full_unstemmed |
Unravelling the changes during induced vitellogenesis in female European eel through RNA-Seq: What happens to the liver? |
title_sort |
unravelling the changes during induced vitellogenesis in female european eel through rna-seq: what happens to the liver? |
publishDate |
2020 |
url |
https://hdl.handle.net/11585/900938 https://doi.org/10.1371/journal.pone.0236438 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0236438 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/32790680 info:eu-repo/semantics/altIdentifier/wos/WOS:000562668300029 volume:15 issue:8 firstpage:1 lastpage:19 numberofpages:19 journal:PLOS ONE https://hdl.handle.net/11585/900938 doi:10.1371/journal.pone.0236438 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85089537670 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0236438 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0236438 |
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PLOS ONE |
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