Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions
The Moon is characterised by extremely harsh conditions due to ultraviolet irradiation, wide temperature extremes, vacuum resulting from the absence of an atmosphere, and high ionising radiation. Therefore, its surface may provide a unique platform to investigate the effects of such conditions. For...
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ftunibolognairis:oai:cris.unibo.it:11585/880731 2024-09-15T17:44:44+00:00 Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions Cassaro A. Pacelli C. Baqué M. Cavalazzi B. Gasparotto G. Saladino R. Botta L. Böttger U. Rabbow E. de Vera J. P. Onofri S. Cassaro A., Pacelli C., Baqué M., Cavalazzi B., Gasparotto G., Saladino R., Botta L., Böttger U., Rabbow E., de Vera J.P., Onofri S. 2022 ELETTRONICO http://hdl.handle.net/11585/880731 https://doi.org/10.1111/1462-2920.15995 https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.15995 eng eng info:eu-repo/semantics/altIdentifier/pmid/35437941 info:eu-repo/semantics/altIdentifier/wos/WOS:000785887700001 volume:24 issue:7 firstpage:2938 lastpage:2950 numberofpages:13 journal:ENVIRONMENTAL MICROBIOLOGY info:eu-repo/grantAgreement/EC/H2020/871149 http://hdl.handle.net/11585/880731 doi:10.1111/1462-2920.15995 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85128706153 https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.15995 info:eu-repo/semantics/openAccess Biomarkers Deep Space Gateway Moon Lunar soil Apollo 17 info:eu-repo/semantics/article 2022 ftunibolognairis https://doi.org/10.1111/1462-2920.15995 2024-07-01T14:08:31Z The Moon is characterised by extremely harsh conditions due to ultraviolet irradiation, wide temperature extremes, vacuum resulting from the absence of an atmosphere, and high ionising radiation. Therefore, its surface may provide a unique platform to investigate the effects of such conditions. For lunar exploration with the Lunar Gateway platform, exposure experiments in Low Earth Orbit are useful testbeds to prepare for lunar space experiments and to understand how and if potential biomarkers are influenced by extra-terrestrial conditions. During the BIOMEX (BIOlogy and Mars EXperiment) project, dried colonies of the fungus Cryomyces antarcticus grown on Lunar Regolith Analogue (LRA) were exposed to space conditions for 16 months aboard the EXPOSE-R2 payload outside the International Space Station. In this study, we investigated the stability/degradation of fungal biomarkers in LRA after exposure to i) simulated space and ii) real space conditions, using Raman spectroscopy, gas chromatography-mass spectrometry, and DNA amplification. The results demonstrated that fungal biomarkers were detectable after 16 months of real space exposure. This work will contribute to the interpretation of data from future biological experiments in the Cislunar orbit with the Lunar Gateway platform and/or on the lunar surface, in preparation for the next step of human exploration Article in Journal/Newspaper Antarc* antarcticus IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) Environmental Microbiology 24 7 2938 2950 |
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Open Polar |
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IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
op_collection_id |
ftunibolognairis |
language |
English |
topic |
Biomarkers Deep Space Gateway Moon Lunar soil Apollo 17 |
spellingShingle |
Biomarkers Deep Space Gateway Moon Lunar soil Apollo 17 Cassaro A. Pacelli C. Baqué M. Cavalazzi B. Gasparotto G. Saladino R. Botta L. Böttger U. Rabbow E. de Vera J. P. Onofri S. Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions |
topic_facet |
Biomarkers Deep Space Gateway Moon Lunar soil Apollo 17 |
description |
The Moon is characterised by extremely harsh conditions due to ultraviolet irradiation, wide temperature extremes, vacuum resulting from the absence of an atmosphere, and high ionising radiation. Therefore, its surface may provide a unique platform to investigate the effects of such conditions. For lunar exploration with the Lunar Gateway platform, exposure experiments in Low Earth Orbit are useful testbeds to prepare for lunar space experiments and to understand how and if potential biomarkers are influenced by extra-terrestrial conditions. During the BIOMEX (BIOlogy and Mars EXperiment) project, dried colonies of the fungus Cryomyces antarcticus grown on Lunar Regolith Analogue (LRA) were exposed to space conditions for 16 months aboard the EXPOSE-R2 payload outside the International Space Station. In this study, we investigated the stability/degradation of fungal biomarkers in LRA after exposure to i) simulated space and ii) real space conditions, using Raman spectroscopy, gas chromatography-mass spectrometry, and DNA amplification. The results demonstrated that fungal biomarkers were detectable after 16 months of real space exposure. This work will contribute to the interpretation of data from future biological experiments in the Cislunar orbit with the Lunar Gateway platform and/or on the lunar surface, in preparation for the next step of human exploration |
author2 |
Cassaro A., Pacelli C., Baqué M., Cavalazzi B., Gasparotto G., Saladino R., Botta L., Böttger U., Rabbow E., de Vera J.P., Onofri S. |
format |
Article in Journal/Newspaper |
author |
Cassaro A. Pacelli C. Baqué M. Cavalazzi B. Gasparotto G. Saladino R. Botta L. Böttger U. Rabbow E. de Vera J. P. Onofri S. |
author_facet |
Cassaro A. Pacelli C. Baqué M. Cavalazzi B. Gasparotto G. Saladino R. Botta L. Böttger U. Rabbow E. de Vera J. P. Onofri S. |
author_sort |
Cassaro A. |
title |
Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions |
title_short |
Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions |
title_full |
Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions |
title_fullStr |
Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions |
title_full_unstemmed |
Investigation of fungal biomolecules after Low Earth Orbit exposure: a testbed for the next Moon missions |
title_sort |
investigation of fungal biomolecules after low earth orbit exposure: a testbed for the next moon missions |
publishDate |
2022 |
url |
http://hdl.handle.net/11585/880731 https://doi.org/10.1111/1462-2920.15995 https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.15995 |
genre |
Antarc* antarcticus |
genre_facet |
Antarc* antarcticus |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/35437941 info:eu-repo/semantics/altIdentifier/wos/WOS:000785887700001 volume:24 issue:7 firstpage:2938 lastpage:2950 numberofpages:13 journal:ENVIRONMENTAL MICROBIOLOGY info:eu-repo/grantAgreement/EC/H2020/871149 http://hdl.handle.net/11585/880731 doi:10.1111/1462-2920.15995 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85128706153 https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.15995 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1111/1462-2920.15995 |
container_title |
Environmental Microbiology |
container_volume |
24 |
container_issue |
7 |
container_start_page |
2938 |
op_container_end_page |
2950 |
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1810492398858403840 |