Alkalizing reactions streamline cellular metabolism in acidogenic microorganisms
expression, gene regulation An understanding of the integrated relationships among the principal cellular functions that govern the bioenergetic reactions of an organism is necessary to determine how cells remain viable and optimise their fitness in the environment. Urease is a complex enzyme that c...
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ftunibolognairis:oai:cris.unibo.it:11585/848654 2024-05-19T07:38:53+00:00 Alkalizing reactions streamline cellular metabolism in acidogenic microorganisms Arioli S Ragg E Scaglioni L Fessas D Signorelli M Karp M Daffonchio D De Noni I Mulas L Oggioni M Guglielmetti S Mora D Arioli S Ragg E Scaglioni L Fessas D Signorelli M Karp M Daffonchio D De Noni I Mulas L Oggioni M Guglielmetti S Mora D 2010 ELETTRONICO http://hdl.handle.net/11585/848654 https://doi.org/10.1371/journal.pone.0015520 eng eng info:eu-repo/semantics/altIdentifier/pmid/21152088 info:eu-repo/semantics/altIdentifier/wos/WOS:000284755100091 volume:5 issue:11 firstpage:1 lastpage:8 numberofpages:8 journal:PLOS ONE http://hdl.handle.net/11585/848654 doi:10.1371/journal.pone.0015520 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-78649790948 Microbiology Metabolism pH regulation info:eu-repo/semantics/article 2010 ftunibolognairis https://doi.org/10.1371/journal.pone.0015520 2024-04-19T00:17:36Z expression, gene regulation An understanding of the integrated relationships among the principal cellular functions that govern the bioenergetic reactions of an organism is necessary to determine how cells remain viable and optimise their fitness in the environment. Urease is a complex enzyme that catalyzes the hydrolysis of urea to ammonia and carbonic acid. While the induction of urease activity by several microorganisms has been predominantly considered a stress-response that is initiated to generate a nitrogen source in response to a low environmental pH, here we demonstrate a new role of urease in the optimisation of cellular bioenergetics. We show that urea hydrolysis increases the catabolic efficiency of Streptococcus thermophilus, a lactic acid bacterium that is widely used in the industrial manufacture of dairy products. By modulating the intracellular pH and thereby increasing the activity of β-galactosidase, glycolytic enzymes and lactate dehydrogenase, urease increases the overall change in enthalpy generated by the bioenergetic reactions. A cooperative altruistic behaviour of urease-positive microorganisms on the urease-negative microorganisms within the same environment was also observed. The physiological role of a single enzymatic activity demonstrates a novel and unexpected view of the non-transcriptional regulatory mechanisms that govern the bioenergetics of a bacterial cell, highlighting a new role for cytosol-alkalizing biochemical pathways in acidogenic microorganisms. Article in Journal/Newspaper Carbonic acid IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) PLoS ONE 5 11 e15520 |
institution |
Open Polar |
collection |
IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
op_collection_id |
ftunibolognairis |
language |
English |
topic |
Microbiology Metabolism pH regulation |
spellingShingle |
Microbiology Metabolism pH regulation Arioli S Ragg E Scaglioni L Fessas D Signorelli M Karp M Daffonchio D De Noni I Mulas L Oggioni M Guglielmetti S Mora D Alkalizing reactions streamline cellular metabolism in acidogenic microorganisms |
topic_facet |
Microbiology Metabolism pH regulation |
description |
expression, gene regulation An understanding of the integrated relationships among the principal cellular functions that govern the bioenergetic reactions of an organism is necessary to determine how cells remain viable and optimise their fitness in the environment. Urease is a complex enzyme that catalyzes the hydrolysis of urea to ammonia and carbonic acid. While the induction of urease activity by several microorganisms has been predominantly considered a stress-response that is initiated to generate a nitrogen source in response to a low environmental pH, here we demonstrate a new role of urease in the optimisation of cellular bioenergetics. We show that urea hydrolysis increases the catabolic efficiency of Streptococcus thermophilus, a lactic acid bacterium that is widely used in the industrial manufacture of dairy products. By modulating the intracellular pH and thereby increasing the activity of β-galactosidase, glycolytic enzymes and lactate dehydrogenase, urease increases the overall change in enthalpy generated by the bioenergetic reactions. A cooperative altruistic behaviour of urease-positive microorganisms on the urease-negative microorganisms within the same environment was also observed. The physiological role of a single enzymatic activity demonstrates a novel and unexpected view of the non-transcriptional regulatory mechanisms that govern the bioenergetics of a bacterial cell, highlighting a new role for cytosol-alkalizing biochemical pathways in acidogenic microorganisms. |
author2 |
Arioli S Ragg E Scaglioni L Fessas D Signorelli M Karp M Daffonchio D De Noni I Mulas L Oggioni M Guglielmetti S Mora D |
format |
Article in Journal/Newspaper |
author |
Arioli S Ragg E Scaglioni L Fessas D Signorelli M Karp M Daffonchio D De Noni I Mulas L Oggioni M Guglielmetti S Mora D |
author_facet |
Arioli S Ragg E Scaglioni L Fessas D Signorelli M Karp M Daffonchio D De Noni I Mulas L Oggioni M Guglielmetti S Mora D |
author_sort |
Arioli S |
title |
Alkalizing reactions streamline cellular metabolism in acidogenic microorganisms |
title_short |
Alkalizing reactions streamline cellular metabolism in acidogenic microorganisms |
title_full |
Alkalizing reactions streamline cellular metabolism in acidogenic microorganisms |
title_fullStr |
Alkalizing reactions streamline cellular metabolism in acidogenic microorganisms |
title_full_unstemmed |
Alkalizing reactions streamline cellular metabolism in acidogenic microorganisms |
title_sort |
alkalizing reactions streamline cellular metabolism in acidogenic microorganisms |
publishDate |
2010 |
url |
http://hdl.handle.net/11585/848654 https://doi.org/10.1371/journal.pone.0015520 |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/21152088 info:eu-repo/semantics/altIdentifier/wos/WOS:000284755100091 volume:5 issue:11 firstpage:1 lastpage:8 numberofpages:8 journal:PLOS ONE http://hdl.handle.net/11585/848654 doi:10.1371/journal.pone.0015520 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-78649790948 |
op_doi |
https://doi.org/10.1371/journal.pone.0015520 |
container_title |
PLoS ONE |
container_volume |
5 |
container_issue |
11 |
container_start_page |
e15520 |
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1799478382685061120 |