Integrated use of molecular techniques to detect and genetically characterise dna viruses in italian wolves (Canis lupus italicus)
In this study, internal organs (tongue, intestine, and spleen) of 23 free-ranging Italian wolves (Canis lupus italicus) found dead between 2017 and 2019 were tested for Carnivore protoparvovirus 1, Canine adenovirus (CAdV), and Canine circovirus (CanineCV) using real-time PCR assays. Genetic charact...
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ftunibolognairis:oai:cris.unibo.it:11585/829668 2024-04-14T08:10:07+00:00 Integrated use of molecular techniques to detect and genetically characterise dna viruses in italian wolves (Canis lupus italicus) Balboni A. Urbani L. Delogu M. Musto C. Fontana M. C. Merialdi G. Lucifora G. Terrusi A. Dondi F. Battilani M. Balboni A. Urbani L. Delogu M. Musto C. Fontana M.C. Merialdi G. Lucifora G. Terrusi A. Dondi F. Battilani M. 2021 ELETTRONICO http://hdl.handle.net/11585/829668 https://doi.org/10.3390/ani11082198 https://www.mdpi.com/2076-2615/11/8/2198/htm eng eng info:eu-repo/semantics/altIdentifier/pmid/34438655 info:eu-repo/semantics/altIdentifier/wos/WOS:000688741400001 volume:11 issue:8 firstpage:1 lastpage:15 numberofpages:15 journal:ANIMALS http://hdl.handle.net/11585/829668 doi:10.3390/ani11082198 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85111011673 https://www.mdpi.com/2076-2615/11/8/2198/htm info:eu-repo/semantics/openAccess Canine adenoviru Canine circoviru Carnivore protoparvovirus 1 Italy Phylogeny Wolf info:eu-repo/semantics/article 2021 ftunibolognairis https://doi.org/10.3390/ani11082198 2024-03-21T18:43:15Z In this study, internal organs (tongue, intestine, and spleen) of 23 free-ranging Italian wolves (Canis lupus italicus) found dead between 2017 and 2019 were tested for Carnivore protoparvovirus 1, Canine adenovirus (CAdV), and Canine circovirus (CanineCV) using real-time PCR assays. Genetic characterisation of the identified viruses was carried out by amplification, sequencing, and analysis of the complete viral genome or informative viral genes. All the wolves tested positive for at least one of the DNA viruses screened, and 11/23 were coinfected. Carnivore protoparvoviruses were the most frequently detected viruses (21/23), followed by CanineCV (11/23) and CAdV (4/23). From the analysis of the partial VP2 gene of 13 carnivore protoparvoviruses, 12 were canine parvovirus type 2b, closely related to the strains detected in dogs and wild carnivores from Italy, and one was a feline panleukopenia-like virus. Of the four CAdV identified, two were CAdV-1 and two were CAdV-2. The complete genome of seven CanineCVs was sequenced and related to the CanineCV identified in dogs, wolves, and foxes worldwide. Close correlations emerged between the viruses identified in wolves and those circulating in domestic dogs. Further studies are needed to investigate if these pathogens may be potentially cross-transmitted between the two species. Article in Journal/Newspaper Canis lupus IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) Animals 11 8 2198 |
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Open Polar |
collection |
IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
op_collection_id |
ftunibolognairis |
language |
English |
topic |
Canine adenoviru Canine circoviru Carnivore protoparvovirus 1 Italy Phylogeny Wolf |
spellingShingle |
Canine adenoviru Canine circoviru Carnivore protoparvovirus 1 Italy Phylogeny Wolf Balboni A. Urbani L. Delogu M. Musto C. Fontana M. C. Merialdi G. Lucifora G. Terrusi A. Dondi F. Battilani M. Integrated use of molecular techniques to detect and genetically characterise dna viruses in italian wolves (Canis lupus italicus) |
topic_facet |
Canine adenoviru Canine circoviru Carnivore protoparvovirus 1 Italy Phylogeny Wolf |
description |
In this study, internal organs (tongue, intestine, and spleen) of 23 free-ranging Italian wolves (Canis lupus italicus) found dead between 2017 and 2019 were tested for Carnivore protoparvovirus 1, Canine adenovirus (CAdV), and Canine circovirus (CanineCV) using real-time PCR assays. Genetic characterisation of the identified viruses was carried out by amplification, sequencing, and analysis of the complete viral genome or informative viral genes. All the wolves tested positive for at least one of the DNA viruses screened, and 11/23 were coinfected. Carnivore protoparvoviruses were the most frequently detected viruses (21/23), followed by CanineCV (11/23) and CAdV (4/23). From the analysis of the partial VP2 gene of 13 carnivore protoparvoviruses, 12 were canine parvovirus type 2b, closely related to the strains detected in dogs and wild carnivores from Italy, and one was a feline panleukopenia-like virus. Of the four CAdV identified, two were CAdV-1 and two were CAdV-2. The complete genome of seven CanineCVs was sequenced and related to the CanineCV identified in dogs, wolves, and foxes worldwide. Close correlations emerged between the viruses identified in wolves and those circulating in domestic dogs. Further studies are needed to investigate if these pathogens may be potentially cross-transmitted between the two species. |
author2 |
Balboni A. Urbani L. Delogu M. Musto C. Fontana M.C. Merialdi G. Lucifora G. Terrusi A. Dondi F. Battilani M. |
format |
Article in Journal/Newspaper |
author |
Balboni A. Urbani L. Delogu M. Musto C. Fontana M. C. Merialdi G. Lucifora G. Terrusi A. Dondi F. Battilani M. |
author_facet |
Balboni A. Urbani L. Delogu M. Musto C. Fontana M. C. Merialdi G. Lucifora G. Terrusi A. Dondi F. Battilani M. |
author_sort |
Balboni A. |
title |
Integrated use of molecular techniques to detect and genetically characterise dna viruses in italian wolves (Canis lupus italicus) |
title_short |
Integrated use of molecular techniques to detect and genetically characterise dna viruses in italian wolves (Canis lupus italicus) |
title_full |
Integrated use of molecular techniques to detect and genetically characterise dna viruses in italian wolves (Canis lupus italicus) |
title_fullStr |
Integrated use of molecular techniques to detect and genetically characterise dna viruses in italian wolves (Canis lupus italicus) |
title_full_unstemmed |
Integrated use of molecular techniques to detect and genetically characterise dna viruses in italian wolves (Canis lupus italicus) |
title_sort |
integrated use of molecular techniques to detect and genetically characterise dna viruses in italian wolves (canis lupus italicus) |
publishDate |
2021 |
url |
http://hdl.handle.net/11585/829668 https://doi.org/10.3390/ani11082198 https://www.mdpi.com/2076-2615/11/8/2198/htm |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/34438655 info:eu-repo/semantics/altIdentifier/wos/WOS:000688741400001 volume:11 issue:8 firstpage:1 lastpage:15 numberofpages:15 journal:ANIMALS http://hdl.handle.net/11585/829668 doi:10.3390/ani11082198 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85111011673 https://www.mdpi.com/2076-2615/11/8/2198/htm |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/ani11082198 |
container_title |
Animals |
container_volume |
11 |
container_issue |
8 |
container_start_page |
2198 |
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1796307624063401984 |