VASA expression suggests shared germ line dynamics in bivalve molluscs
Germ line segregation can occur during embryogenesis or after embryogenesis completion, with multipotent cells able to give rise to both germ and somatic cells in the developing juvenile or even in adulthood. These undifferentiated cells, in some animals, are self-renewing stem cells. In all these c...
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Online Access: | http://hdl.handle.net/11585/607238 https://doi.org/10.1007/s00418-017-1560-x https://link.springer.com/article/10.1007/s00418-017-1560-x |
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ftunibolognairis:oai:cris.unibo.it:11585/607238 2024-04-14T08:10:45+00:00 VASA expression suggests shared germ line dynamics in bivalve molluscs MILANI, LILIANA PECCI, ANDREA GHISELLI, FABRIZIO PASSAMONTI, MARCO BETTINI, SIMONE FRANCESCHINI, VALERIA MAURIZII, MARIA GABRIELLA Milani, Liliana Pecci, Andrea Ghiselli, Fabrizio Passamonti, Marco Bettini, Simone Franceschini, Valeria Maurizii, Maria Gabriella 2017 ELETTRONICO http://hdl.handle.net/11585/607238 https://doi.org/10.1007/s00418-017-1560-x https://link.springer.com/article/10.1007/s00418-017-1560-x eng eng info:eu-repo/semantics/altIdentifier/pmid/28386635 info:eu-repo/semantics/altIdentifier/wos/WOS:000405559100006 volume:148 issue:2 firstpage:157 lastpage:171 numberofpages:15 journal:HISTOCHEMISTRY AND CELL BIOLOGY http://hdl.handle.net/11585/607238 doi:10.1007/s00418-017-1560-x info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85017173239 https://link.springer.com/article/10.1007/s00418-017-1560-x info:eu-repo/semantics/openAccess Anadara kagoshimensi Crassostrea giga Gonad Immunohistochemistry Multipotent cell Mya arenaria Histology Molecular Biology Medical Laboratory Technology Cell Biology info:eu-repo/semantics/article 2017 ftunibolognairis https://doi.org/10.1007/s00418-017-1560-x 2024-03-21T17:44:01Z Germ line segregation can occur during embryogenesis or after embryogenesis completion, with multipotent cells able to give rise to both germ and somatic cells in the developing juvenile or even in adulthood. These undifferentiated cells, in some animals, are self-renewing stem cells. In all these cell lineages, the same set of genes, among which vasa, appears to be expressed. We traced VASA expression during the peculiar gonad rebuilding of bivalves to verify its presence from undifferentiated germ cells to mature gametes in an animal taxon in which the mechanism of germ line establishment is still under investigation. We utilized antibodies produced against VASPH, VASA homolog of Ruditapes philippinarum (Subclass Heterodonta), to compare the known expression pattern of R. philippinarum to two species of the Subclass Pteriomorphia, Anadara kagoshimensis and Crassostrea gigas, and another species of the Subclass Heterodonta, Mya arenaria. The immunohistological data obtained support a conserved mechanism of proliferation of “primordial stem cells†among the simple columnar epithelium of the gut, as well as in the connective tissue, contributing to the seasonal gonad reconstitution. Given the taxonomic separation of the analyzed species, we suggest that the process could be shared in bivalve molluscs. The presence of germ cell precursors in the gut epithelium appears to be a feature in common with model organisms, such as mouse, fruit fly, and human. Thus, the comparative study of germ line establishment can add details on bivalve development, but can also help to clarify the role that VASA plays during germ cell specification. Article in Journal/Newspaper Crassostrea gigas IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) Vasa ENVELOPE(25.177,25.177,67.587,67.587) Histochemistry and Cell Biology 148 2 157 171 |
institution |
Open Polar |
collection |
IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
op_collection_id |
ftunibolognairis |
language |
English |
topic |
Anadara kagoshimensi Crassostrea giga Gonad Immunohistochemistry Multipotent cell Mya arenaria Histology Molecular Biology Medical Laboratory Technology Cell Biology |
spellingShingle |
Anadara kagoshimensi Crassostrea giga Gonad Immunohistochemistry Multipotent cell Mya arenaria Histology Molecular Biology Medical Laboratory Technology Cell Biology MILANI, LILIANA PECCI, ANDREA GHISELLI, FABRIZIO PASSAMONTI, MARCO BETTINI, SIMONE FRANCESCHINI, VALERIA MAURIZII, MARIA GABRIELLA VASA expression suggests shared germ line dynamics in bivalve molluscs |
topic_facet |
Anadara kagoshimensi Crassostrea giga Gonad Immunohistochemistry Multipotent cell Mya arenaria Histology Molecular Biology Medical Laboratory Technology Cell Biology |
description |
Germ line segregation can occur during embryogenesis or after embryogenesis completion, with multipotent cells able to give rise to both germ and somatic cells in the developing juvenile or even in adulthood. These undifferentiated cells, in some animals, are self-renewing stem cells. In all these cell lineages, the same set of genes, among which vasa, appears to be expressed. We traced VASA expression during the peculiar gonad rebuilding of bivalves to verify its presence from undifferentiated germ cells to mature gametes in an animal taxon in which the mechanism of germ line establishment is still under investigation. We utilized antibodies produced against VASPH, VASA homolog of Ruditapes philippinarum (Subclass Heterodonta), to compare the known expression pattern of R. philippinarum to two species of the Subclass Pteriomorphia, Anadara kagoshimensis and Crassostrea gigas, and another species of the Subclass Heterodonta, Mya arenaria. The immunohistological data obtained support a conserved mechanism of proliferation of “primordial stem cells†among the simple columnar epithelium of the gut, as well as in the connective tissue, contributing to the seasonal gonad reconstitution. Given the taxonomic separation of the analyzed species, we suggest that the process could be shared in bivalve molluscs. The presence of germ cell precursors in the gut epithelium appears to be a feature in common with model organisms, such as mouse, fruit fly, and human. Thus, the comparative study of germ line establishment can add details on bivalve development, but can also help to clarify the role that VASA plays during germ cell specification. |
author2 |
Milani, Liliana Pecci, Andrea Ghiselli, Fabrizio Passamonti, Marco Bettini, Simone Franceschini, Valeria Maurizii, Maria Gabriella |
format |
Article in Journal/Newspaper |
author |
MILANI, LILIANA PECCI, ANDREA GHISELLI, FABRIZIO PASSAMONTI, MARCO BETTINI, SIMONE FRANCESCHINI, VALERIA MAURIZII, MARIA GABRIELLA |
author_facet |
MILANI, LILIANA PECCI, ANDREA GHISELLI, FABRIZIO PASSAMONTI, MARCO BETTINI, SIMONE FRANCESCHINI, VALERIA MAURIZII, MARIA GABRIELLA |
author_sort |
MILANI, LILIANA |
title |
VASA expression suggests shared germ line dynamics in bivalve molluscs |
title_short |
VASA expression suggests shared germ line dynamics in bivalve molluscs |
title_full |
VASA expression suggests shared germ line dynamics in bivalve molluscs |
title_fullStr |
VASA expression suggests shared germ line dynamics in bivalve molluscs |
title_full_unstemmed |
VASA expression suggests shared germ line dynamics in bivalve molluscs |
title_sort |
vasa expression suggests shared germ line dynamics in bivalve molluscs |
publishDate |
2017 |
url |
http://hdl.handle.net/11585/607238 https://doi.org/10.1007/s00418-017-1560-x https://link.springer.com/article/10.1007/s00418-017-1560-x |
long_lat |
ENVELOPE(25.177,25.177,67.587,67.587) |
geographic |
Vasa |
geographic_facet |
Vasa |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/28386635 info:eu-repo/semantics/altIdentifier/wos/WOS:000405559100006 volume:148 issue:2 firstpage:157 lastpage:171 numberofpages:15 journal:HISTOCHEMISTRY AND CELL BIOLOGY http://hdl.handle.net/11585/607238 doi:10.1007/s00418-017-1560-x info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85017173239 https://link.springer.com/article/10.1007/s00418-017-1560-x |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s00418-017-1560-x |
container_title |
Histochemistry and Cell Biology |
container_volume |
148 |
container_issue |
2 |
container_start_page |
157 |
op_container_end_page |
171 |
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1796308416983990272 |