Selection of best-performing reference gene products for investigating transcriptional regulation across silvering in the European eel (Anguilla anguilla)
The focus of the present study was to set a methodological approach for evaluating molecular mechanisms underlying silvering transformation in the European eel, Anguilla anguilla. Silvering is a tightly controlled process during which eels undergo significant morphological, physiological and behavio...
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Online Access: | http://hdl.handle.net/11585/523538 https://doi.org/10.1038/srep16966 |
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ftunibolognairis:oai:cris.unibo.it:11585/523538 2024-09-09T19:00:29+00:00 Selection of best-performing reference gene products for investigating transcriptional regulation across silvering in the European eel (Anguilla anguilla) FRANZELLITTI, SILVIA KIWAN, ALISAR VALBONESI, PAOLA FABBRI, ELENA Franzellitti, Silvia Kiwan, Alisar Valbonesi, Paola Fabbri, Elena 2015 STAMPA http://hdl.handle.net/11585/523538 https://doi.org/10.1038/srep16966 eng eng info:eu-repo/semantics/altIdentifier/pmid/26593703 info:eu-repo/semantics/altIdentifier/wos/WOS:000365239300002 volume:5:16966 firstpage:1 lastpage:14 numberofpages:14 journal:SCIENTIFIC REPORTS http://hdl.handle.net/11585/523538 doi:10.1038/srep16966 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84948171614 Anguilla anguilla gene expression silvering info:eu-repo/semantics/article 2015 ftunibolognairis https://doi.org/10.1038/srep16966 2024-07-08T14:06:46Z The focus of the present study was to set a methodological approach for evaluating molecular mechanisms underlying silvering transformation in the European eel, Anguilla anguilla. Silvering is a tightly controlled process during which eels undergo significant morphological, physiological and behavioral changes, pre-adapting for the oceanic spawning migration. Female eels showing different silver indexes were caught in different seasons in the Comacchio Lagoon (North Adriatic Sea, Italy). Isolated hepatocytes from these eels were selected as the experimental model given the relevant role of these cells in metabolic functions potentially altered during silvering. Expression profiles of 7 candidate reference transcripts were analyzed seeking the most viable and robust strategies for accurate qPCR data normalization during silvering. Stability analysis and further statistical validation identified transcripts encoding the ribosomal proteins L13 and ARP as the appropriate reference genes in studies on A. anguilla through silvering. The identified reference transcripts were further used to evaluate expression profiles of target transcripts encoding the thyroid hormone receptor β (THRβ) and vitellogenin (vtg), known to be involved in silvering processes. To the best of our knowledge, this is the first study comparing THRβ expression in European eels across silvering. Article in Journal/Newspaper Anguilla anguilla IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) Scientific Reports 5 1 |
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Open Polar |
collection |
IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
op_collection_id |
ftunibolognairis |
language |
English |
topic |
Anguilla anguilla gene expression silvering |
spellingShingle |
Anguilla anguilla gene expression silvering FRANZELLITTI, SILVIA KIWAN, ALISAR VALBONESI, PAOLA FABBRI, ELENA Selection of best-performing reference gene products for investigating transcriptional regulation across silvering in the European eel (Anguilla anguilla) |
topic_facet |
Anguilla anguilla gene expression silvering |
description |
The focus of the present study was to set a methodological approach for evaluating molecular mechanisms underlying silvering transformation in the European eel, Anguilla anguilla. Silvering is a tightly controlled process during which eels undergo significant morphological, physiological and behavioral changes, pre-adapting for the oceanic spawning migration. Female eels showing different silver indexes were caught in different seasons in the Comacchio Lagoon (North Adriatic Sea, Italy). Isolated hepatocytes from these eels were selected as the experimental model given the relevant role of these cells in metabolic functions potentially altered during silvering. Expression profiles of 7 candidate reference transcripts were analyzed seeking the most viable and robust strategies for accurate qPCR data normalization during silvering. Stability analysis and further statistical validation identified transcripts encoding the ribosomal proteins L13 and ARP as the appropriate reference genes in studies on A. anguilla through silvering. The identified reference transcripts were further used to evaluate expression profiles of target transcripts encoding the thyroid hormone receptor β (THRβ) and vitellogenin (vtg), known to be involved in silvering processes. To the best of our knowledge, this is the first study comparing THRβ expression in European eels across silvering. |
author2 |
Franzellitti, Silvia Kiwan, Alisar Valbonesi, Paola Fabbri, Elena |
format |
Article in Journal/Newspaper |
author |
FRANZELLITTI, SILVIA KIWAN, ALISAR VALBONESI, PAOLA FABBRI, ELENA |
author_facet |
FRANZELLITTI, SILVIA KIWAN, ALISAR VALBONESI, PAOLA FABBRI, ELENA |
author_sort |
FRANZELLITTI, SILVIA |
title |
Selection of best-performing reference gene products for investigating transcriptional regulation across silvering in the European eel (Anguilla anguilla) |
title_short |
Selection of best-performing reference gene products for investigating transcriptional regulation across silvering in the European eel (Anguilla anguilla) |
title_full |
Selection of best-performing reference gene products for investigating transcriptional regulation across silvering in the European eel (Anguilla anguilla) |
title_fullStr |
Selection of best-performing reference gene products for investigating transcriptional regulation across silvering in the European eel (Anguilla anguilla) |
title_full_unstemmed |
Selection of best-performing reference gene products for investigating transcriptional regulation across silvering in the European eel (Anguilla anguilla) |
title_sort |
selection of best-performing reference gene products for investigating transcriptional regulation across silvering in the european eel (anguilla anguilla) |
publishDate |
2015 |
url |
http://hdl.handle.net/11585/523538 https://doi.org/10.1038/srep16966 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/26593703 info:eu-repo/semantics/altIdentifier/wos/WOS:000365239300002 volume:5:16966 firstpage:1 lastpage:14 numberofpages:14 journal:SCIENTIFIC REPORTS http://hdl.handle.net/11585/523538 doi:10.1038/srep16966 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84948171614 |
op_doi |
https://doi.org/10.1038/srep16966 |
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Scientific Reports |
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1809939681030176768 |