Adrenergic receptors and signaling in yellow and silver European eel hepatocytes
European eels spend up to twenty years in fresh or brackish water as “yellow” eels. Then, part of the population change into migrant “silver” eels, moving towards the spawning grounds at the Sargasso sea. Migrating eels do not feed, and show higher levels of cortisol, causing lipolysis in muscle and...
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ftunibolognairis:oai:cris.unibo.it:11585/393723 2024-04-14T08:01:02+00:00 Adrenergic receptors and signaling in yellow and silver European eel hepatocytes KIWAN, ALISAR FRANZELLITTI, SILVIA VALBONESI, PAOLA FABBRI, ELENA Alisar Kiwan Silvia Franzellitti Paola Valbonesi Elena Fabbri 2013 ELETTRONICO http://hdl.handle.net/11585/393723 eng eng ispartofbook:64°Congresso Nazionale 2013 - Società Italiana di Fisiologia - Book of abstract Società Italiana di Fisiologia (SIF) firstpage:134 lastpage:134 numberofpages:1 http://hdl.handle.net/11585/393723 EUROPEAN EEL (ANGUILLA ANGUILLA) ADRENERGIC RECEPTORS Hepatocyte CATECHOLAMINES info:eu-repo/semantics/conferenceObject 2013 ftunibolognairis 2024-03-21T17:28:58Z European eels spend up to twenty years in fresh or brackish water as “yellow” eels. Then, part of the population change into migrant “silver” eels, moving towards the spawning grounds at the Sargasso sea. Migrating eels do not feed, and show higher levels of cortisol, causing lipolysis in muscle and liver and higher free fatty acids into the blood. Further hormones change their levels and modulate metabolism in migrating eels, including insulin, IGFs, leptin, and ghrelin. Our investigation compared for the first time catecholamine regulation of liver metabolism in yellow and silver eels. Expression of α1- and β2-adrenergic receptor (AR) mRNAs in eel hepatocytes was assessed by qRT-PCR. Levels of both α1- and β2-AR mRNAs were significantly higher in hepatocytes from silver than from yellow eels, with a 5-fold and a 2-fold increase, respectively. As a result, the mean ratio α1-AR/β2-AR transcripts was significantly higher in silver than in yellow eel. In a first approach, the β2-AR coupled signal transduction was investigated. Epinephrine dose-dependently increased cAMP levels in eel hepatocytes, an effect counteracted by propranolol. Under the same stimuli, the extent of cAMP increase was similar at the two life-stages; differently, the induced glucose release was significantly higher in silver than in yellow eels. Since α1-AR mediated pathway concurs to modulate liver glycogenolysis, further studies will evaluate the contribution of calcium signaling in silver eel response. Conference Object Anguilla anguilla IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
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IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
op_collection_id |
ftunibolognairis |
language |
English |
topic |
EUROPEAN EEL (ANGUILLA ANGUILLA) ADRENERGIC RECEPTORS Hepatocyte CATECHOLAMINES |
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EUROPEAN EEL (ANGUILLA ANGUILLA) ADRENERGIC RECEPTORS Hepatocyte CATECHOLAMINES KIWAN, ALISAR FRANZELLITTI, SILVIA VALBONESI, PAOLA FABBRI, ELENA Adrenergic receptors and signaling in yellow and silver European eel hepatocytes |
topic_facet |
EUROPEAN EEL (ANGUILLA ANGUILLA) ADRENERGIC RECEPTORS Hepatocyte CATECHOLAMINES |
description |
European eels spend up to twenty years in fresh or brackish water as “yellow” eels. Then, part of the population change into migrant “silver” eels, moving towards the spawning grounds at the Sargasso sea. Migrating eels do not feed, and show higher levels of cortisol, causing lipolysis in muscle and liver and higher free fatty acids into the blood. Further hormones change their levels and modulate metabolism in migrating eels, including insulin, IGFs, leptin, and ghrelin. Our investigation compared for the first time catecholamine regulation of liver metabolism in yellow and silver eels. Expression of α1- and β2-adrenergic receptor (AR) mRNAs in eel hepatocytes was assessed by qRT-PCR. Levels of both α1- and β2-AR mRNAs were significantly higher in hepatocytes from silver than from yellow eels, with a 5-fold and a 2-fold increase, respectively. As a result, the mean ratio α1-AR/β2-AR transcripts was significantly higher in silver than in yellow eel. In a first approach, the β2-AR coupled signal transduction was investigated. Epinephrine dose-dependently increased cAMP levels in eel hepatocytes, an effect counteracted by propranolol. Under the same stimuli, the extent of cAMP increase was similar at the two life-stages; differently, the induced glucose release was significantly higher in silver than in yellow eels. Since α1-AR mediated pathway concurs to modulate liver glycogenolysis, further studies will evaluate the contribution of calcium signaling in silver eel response. |
author2 |
Alisar Kiwan Silvia Franzellitti Paola Valbonesi Elena Fabbri |
format |
Conference Object |
author |
KIWAN, ALISAR FRANZELLITTI, SILVIA VALBONESI, PAOLA FABBRI, ELENA |
author_facet |
KIWAN, ALISAR FRANZELLITTI, SILVIA VALBONESI, PAOLA FABBRI, ELENA |
author_sort |
KIWAN, ALISAR |
title |
Adrenergic receptors and signaling in yellow and silver European eel hepatocytes |
title_short |
Adrenergic receptors and signaling in yellow and silver European eel hepatocytes |
title_full |
Adrenergic receptors and signaling in yellow and silver European eel hepatocytes |
title_fullStr |
Adrenergic receptors and signaling in yellow and silver European eel hepatocytes |
title_full_unstemmed |
Adrenergic receptors and signaling in yellow and silver European eel hepatocytes |
title_sort |
adrenergic receptors and signaling in yellow and silver european eel hepatocytes |
publishDate |
2013 |
url |
http://hdl.handle.net/11585/393723 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
ispartofbook:64°Congresso Nazionale 2013 - Società Italiana di Fisiologia - Book of abstract Società Italiana di Fisiologia (SIF) firstpage:134 lastpage:134 numberofpages:1 http://hdl.handle.net/11585/393723 |
_version_ |
1796306670256652288 |