Heat tolerance and gene expression responses to heat stress in threespine sticklebacks from ecologically divergent environments
Ectothermic animals like fishes are extremely dependent on temperature, as they are not able to change body temperature physiologically. When populations are found in isolated water bodies such as small lakes they will have to respond to stressful high temperatures by behavioral avoidance, phenotypi...
Published in: | Journal of Thermal Biology |
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Online Access: | https://pure.au.dk/portal/da/publications/heat-tolerance-and-gene-expression-responses-to-heat-stress-in-threespine-sticklebacks-from-ecologically-divergent-environments(e4863151-5971-40a4-87b8-63218470d3c1).html https://doi.org/10.1016/j.jtherbio.2018.06.003 |
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ftuniaarhuspubl:oai:pure.atira.dk:publications/e4863151-5971-40a4-87b8-63218470d3c1 2023-05-15T15:32:34+02:00 Heat tolerance and gene expression responses to heat stress in threespine sticklebacks from ecologically divergent environments Dammark, Karin Brydso Ferchaud, Anne-Laure Hansen, Michael M. Sorensen, Jesper G. 2018-07 https://pure.au.dk/portal/da/publications/heat-tolerance-and-gene-expression-responses-to-heat-stress-in-threespine-sticklebacks-from-ecologically-divergent-environments(e4863151-5971-40a4-87b8-63218470d3c1).html https://doi.org/10.1016/j.jtherbio.2018.06.003 eng eng info:eu-repo/semantics/restrictedAccess Dammark , K B , Ferchaud , A-L , Hansen , M M & Sorensen , J G 2018 , ' Heat tolerance and gene expression responses to heat stress in threespine sticklebacks from ecologically divergent environments ' , Journal of Thermal Biology , vol. 75 , pp. 88-96 . https://doi.org/10.1016/j.jtherbio.2018.06.003 Climate change Thermal adaptation QPCR CTmax LIFE-HISTORY TRAITS CLIMATE-CHANGE THERMAL TOLERANCE GASTEROSTEUS-ACULEATUS FRESH-WATER ATLANTIC SALMON EVOLUTIONARY RESPONSES FUNDULUS-HETEROCLITUS ADAPTIVE EVOLUTION OXYGEN LIMITATION article 2018 ftuniaarhuspubl https://doi.org/10.1016/j.jtherbio.2018.06.003 2020-07-18T22:07:41Z Ectothermic animals like fishes are extremely dependent on temperature, as they are not able to change body temperature physiologically. When populations are found in isolated water bodies such as small lakes they will have to respond to stressful high temperatures by behavioral avoidance, phenotypic plasticity or microevolutionary change. We analyzed threespine sticklebacks from two large and two small lakes, representing different isolated populations. We determined maximum critical thermal limits (CTmax) and the associated gene expression responses in three heat shock (hsp60, hsp70, hsp90) and two key metabolic (idh2, fbp2) genes at ecologically relevant moderate heat stress (26 degrees C) as well as at the critical thermal limit (CTmax). CTmax showed slight variation across populations with no strong indication of local adaptation. Likewise, there was no strong evidence for local adaptation at the level of gene expression. The expression of the metabolic genes indicated a shift from aerobic towards anaerobic energy production with extreme heat stress. We conclude that threespine sticklebacks do not show severe stress during the warmest temperatures they are likely to encounter during current temperature regimes in Denmark, and following this show no sign of local adaptation even in small, isolated water bodies. Article in Journal/Newspaper Atlantic salmon Aarhus University: Research Journal of Thermal Biology 75 88 96 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
Climate change Thermal adaptation QPCR CTmax LIFE-HISTORY TRAITS CLIMATE-CHANGE THERMAL TOLERANCE GASTEROSTEUS-ACULEATUS FRESH-WATER ATLANTIC SALMON EVOLUTIONARY RESPONSES FUNDULUS-HETEROCLITUS ADAPTIVE EVOLUTION OXYGEN LIMITATION |
spellingShingle |
Climate change Thermal adaptation QPCR CTmax LIFE-HISTORY TRAITS CLIMATE-CHANGE THERMAL TOLERANCE GASTEROSTEUS-ACULEATUS FRESH-WATER ATLANTIC SALMON EVOLUTIONARY RESPONSES FUNDULUS-HETEROCLITUS ADAPTIVE EVOLUTION OXYGEN LIMITATION Dammark, Karin Brydso Ferchaud, Anne-Laure Hansen, Michael M. Sorensen, Jesper G. Heat tolerance and gene expression responses to heat stress in threespine sticklebacks from ecologically divergent environments |
topic_facet |
Climate change Thermal adaptation QPCR CTmax LIFE-HISTORY TRAITS CLIMATE-CHANGE THERMAL TOLERANCE GASTEROSTEUS-ACULEATUS FRESH-WATER ATLANTIC SALMON EVOLUTIONARY RESPONSES FUNDULUS-HETEROCLITUS ADAPTIVE EVOLUTION OXYGEN LIMITATION |
description |
Ectothermic animals like fishes are extremely dependent on temperature, as they are not able to change body temperature physiologically. When populations are found in isolated water bodies such as small lakes they will have to respond to stressful high temperatures by behavioral avoidance, phenotypic plasticity or microevolutionary change. We analyzed threespine sticklebacks from two large and two small lakes, representing different isolated populations. We determined maximum critical thermal limits (CTmax) and the associated gene expression responses in three heat shock (hsp60, hsp70, hsp90) and two key metabolic (idh2, fbp2) genes at ecologically relevant moderate heat stress (26 degrees C) as well as at the critical thermal limit (CTmax). CTmax showed slight variation across populations with no strong indication of local adaptation. Likewise, there was no strong evidence for local adaptation at the level of gene expression. The expression of the metabolic genes indicated a shift from aerobic towards anaerobic energy production with extreme heat stress. We conclude that threespine sticklebacks do not show severe stress during the warmest temperatures they are likely to encounter during current temperature regimes in Denmark, and following this show no sign of local adaptation even in small, isolated water bodies. |
format |
Article in Journal/Newspaper |
author |
Dammark, Karin Brydso Ferchaud, Anne-Laure Hansen, Michael M. Sorensen, Jesper G. |
author_facet |
Dammark, Karin Brydso Ferchaud, Anne-Laure Hansen, Michael M. Sorensen, Jesper G. |
author_sort |
Dammark, Karin Brydso |
title |
Heat tolerance and gene expression responses to heat stress in threespine sticklebacks from ecologically divergent environments |
title_short |
Heat tolerance and gene expression responses to heat stress in threespine sticklebacks from ecologically divergent environments |
title_full |
Heat tolerance and gene expression responses to heat stress in threespine sticklebacks from ecologically divergent environments |
title_fullStr |
Heat tolerance and gene expression responses to heat stress in threespine sticklebacks from ecologically divergent environments |
title_full_unstemmed |
Heat tolerance and gene expression responses to heat stress in threespine sticklebacks from ecologically divergent environments |
title_sort |
heat tolerance and gene expression responses to heat stress in threespine sticklebacks from ecologically divergent environments |
publishDate |
2018 |
url |
https://pure.au.dk/portal/da/publications/heat-tolerance-and-gene-expression-responses-to-heat-stress-in-threespine-sticklebacks-from-ecologically-divergent-environments(e4863151-5971-40a4-87b8-63218470d3c1).html https://doi.org/10.1016/j.jtherbio.2018.06.003 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Dammark , K B , Ferchaud , A-L , Hansen , M M & Sorensen , J G 2018 , ' Heat tolerance and gene expression responses to heat stress in threespine sticklebacks from ecologically divergent environments ' , Journal of Thermal Biology , vol. 75 , pp. 88-96 . https://doi.org/10.1016/j.jtherbio.2018.06.003 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.jtherbio.2018.06.003 |
container_title |
Journal of Thermal Biology |
container_volume |
75 |
container_start_page |
88 |
op_container_end_page |
96 |
_version_ |
1766363056769597440 |