Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment
Microorganisms in marine sediments play major roles in marine biogeochemical cycles by mineralizing substantial quantities of organic matter from decaying cells. Proteins and lipids are abundant components of necromass, yet the taxonomic identities of microorganisms that actively degrade them remain...
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2021
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Online Access: | https://pure.au.dk/portal/da/publications/anaerobic-bacterial-degradation-of-protein-and-lipid-macromolecules-in-subarctic-marine-sediment(d44011dc-c109-4f5b-8906-bf84763bdbe6).html https://doi.org/10.1038/s41396-020-00817-6 https://pure.au.dk/ws/files/285213589/s41396_020_00817_6.pdf http://www.scopus.com/inward/record.url?scp=85096238074&partnerID=8YFLogxK |
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ftuniaarhuspubl:oai:pure.atira.dk:publications/d44011dc-c109-4f5b-8906-bf84763bdbe6 2023-09-05T13:23:36+02:00 Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment Pelikan, Claus Wasmund, Kenneth Glombitza, Clemens Hausmann, Bela Herbold, Craig W. Flieder, Mathias Loy, Alexander 2021-03 application/pdf https://pure.au.dk/portal/da/publications/anaerobic-bacterial-degradation-of-protein-and-lipid-macromolecules-in-subarctic-marine-sediment(d44011dc-c109-4f5b-8906-bf84763bdbe6).html https://doi.org/10.1038/s41396-020-00817-6 https://pure.au.dk/ws/files/285213589/s41396_020_00817_6.pdf http://www.scopus.com/inward/record.url?scp=85096238074&partnerID=8YFLogxK eng eng info:eu-repo/semantics/openAccess Pelikan , C , Wasmund , K , Glombitza , C , Hausmann , B , Herbold , C W , Flieder , M & Loy , A 2021 , ' Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment ' , ISME Journal , vol. 15 , no. 3 , pp. 833-847 . https://doi.org/10.1038/s41396-020-00817-6 article 2021 ftuniaarhuspubl https://doi.org/10.1038/s41396-020-00817-6 2023-08-23T22:58:18Z Microorganisms in marine sediments play major roles in marine biogeochemical cycles by mineralizing substantial quantities of organic matter from decaying cells. Proteins and lipids are abundant components of necromass, yet the taxonomic identities of microorganisms that actively degrade them remain poorly resolved. Here, we revealed identities, trophic interactions, and genomic features of bacteria that degraded 13 C-labeled proteins and lipids in cold anoxic microcosms containing sulfidic subarctic marine sediment. Supplemented proteins and lipids were rapidly fermented to various volatile fatty acids within 5 days. DNA-stable isotope probing (SIP) suggested Psychrilyobacter atlanticus was an important primary degrader of proteins, and Psychromonas members were important primary degraders of both proteins and lipids. Closely related Psychromonas populations, as represented by distinct 16S rRNA gene variants, differentially utilized either proteins or lipids. DNA-SIP also showed 13 C-labeling of various Deltaproteobacteria within 10 days, indicating trophic transfer of carbon to putative sulfate-reducers. Metagenome-assembled genomes revealed the primary hydrolyzers encoded secreted peptidases or lipases, and enzymes for catabolism of protein or lipid degradation products. Psychromonas species are prevalent in diverse marine sediments, suggesting they are important players in organic carbon processing in situ. Together, this study provides new insights into the identities, functions, and genomes of bacteria that actively degrade abundant necromass macromolecules in the seafloor. Article in Journal/Newspaper Subarctic Aarhus University: Research The ISME Journal 15 3 833 847 |
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Aarhus University: Research |
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ftuniaarhuspubl |
language |
English |
description |
Microorganisms in marine sediments play major roles in marine biogeochemical cycles by mineralizing substantial quantities of organic matter from decaying cells. Proteins and lipids are abundant components of necromass, yet the taxonomic identities of microorganisms that actively degrade them remain poorly resolved. Here, we revealed identities, trophic interactions, and genomic features of bacteria that degraded 13 C-labeled proteins and lipids in cold anoxic microcosms containing sulfidic subarctic marine sediment. Supplemented proteins and lipids were rapidly fermented to various volatile fatty acids within 5 days. DNA-stable isotope probing (SIP) suggested Psychrilyobacter atlanticus was an important primary degrader of proteins, and Psychromonas members were important primary degraders of both proteins and lipids. Closely related Psychromonas populations, as represented by distinct 16S rRNA gene variants, differentially utilized either proteins or lipids. DNA-SIP also showed 13 C-labeling of various Deltaproteobacteria within 10 days, indicating trophic transfer of carbon to putative sulfate-reducers. Metagenome-assembled genomes revealed the primary hydrolyzers encoded secreted peptidases or lipases, and enzymes for catabolism of protein or lipid degradation products. Psychromonas species are prevalent in diverse marine sediments, suggesting they are important players in organic carbon processing in situ. Together, this study provides new insights into the identities, functions, and genomes of bacteria that actively degrade abundant necromass macromolecules in the seafloor. |
format |
Article in Journal/Newspaper |
author |
Pelikan, Claus Wasmund, Kenneth Glombitza, Clemens Hausmann, Bela Herbold, Craig W. Flieder, Mathias Loy, Alexander |
spellingShingle |
Pelikan, Claus Wasmund, Kenneth Glombitza, Clemens Hausmann, Bela Herbold, Craig W. Flieder, Mathias Loy, Alexander Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment |
author_facet |
Pelikan, Claus Wasmund, Kenneth Glombitza, Clemens Hausmann, Bela Herbold, Craig W. Flieder, Mathias Loy, Alexander |
author_sort |
Pelikan, Claus |
title |
Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment |
title_short |
Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment |
title_full |
Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment |
title_fullStr |
Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment |
title_full_unstemmed |
Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment |
title_sort |
anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment |
publishDate |
2021 |
url |
https://pure.au.dk/portal/da/publications/anaerobic-bacterial-degradation-of-protein-and-lipid-macromolecules-in-subarctic-marine-sediment(d44011dc-c109-4f5b-8906-bf84763bdbe6).html https://doi.org/10.1038/s41396-020-00817-6 https://pure.au.dk/ws/files/285213589/s41396_020_00817_6.pdf http://www.scopus.com/inward/record.url?scp=85096238074&partnerID=8YFLogxK |
genre |
Subarctic |
genre_facet |
Subarctic |
op_source |
Pelikan , C , Wasmund , K , Glombitza , C , Hausmann , B , Herbold , C W , Flieder , M & Loy , A 2021 , ' Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment ' , ISME Journal , vol. 15 , no. 3 , pp. 833-847 . https://doi.org/10.1038/s41396-020-00817-6 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1038/s41396-020-00817-6 |
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The ISME Journal |
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15 |
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3 |
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833 |
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847 |
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