Proteomics and lipidomics analyses reveal modulation of lipid metabolism by perfluoroalkyl substances in liver of Atlantic cod ( Gadus morhua )
The aim of the present study was to investigate effects of defined mixtures of polycyclic aromatic hydrocarbons (PAHs) and perfluoroalkyl substances (PFASs), at low, environmentally relevant (1× = L), or high (20× = H) doses, on biological responses in Atlantic cod (Gadus morhua). To this end, farme...
Published in: | Aquatic Toxicology |
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Main Authors: | , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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2020
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Online Access: | https://pure.au.dk/portal/da/publications/proteomics-and-lipidomics-analyses-reveal-modulation-of-lipid-metabolism-by-perfluoroalkyl-substances-in-liver-of-atlantic-cod-gadus-morhua(c31cceac-9269-441e-951b-a24384d1e1b2).html https://doi.org/10.1016/j.aquatox.2020.105590 https://pure.au.dk/ws/files/220123746/1_s2.0_S0166445X20303404_main.pdf http://www.scopus.com/inward/record.url?scp=85090025252&partnerID=8YFLogxK |
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ftuniaarhuspubl:oai:pure.atira.dk:publications/c31cceac-9269-441e-951b-a24384d1e1b2 2023-12-24T10:14:52+01:00 Proteomics and lipidomics analyses reveal modulation of lipid metabolism by perfluoroalkyl substances in liver of Atlantic cod ( Gadus morhua ) Dale, Karina Yadetie, Fekadu Müller, Mette Bjørge Pampanin, Daniela M. Gilabert, Alejandra Zhang, Xiaokang Tairova, Zhanna Haarr, Ane Lille-Langøy, Roger Lyche, Jan Ludvig Porte, Cinta Karlsen, Odd André Goksøyr, Anders 2020-10 application/pdf https://pure.au.dk/portal/da/publications/proteomics-and-lipidomics-analyses-reveal-modulation-of-lipid-metabolism-by-perfluoroalkyl-substances-in-liver-of-atlantic-cod-gadus-morhua(c31cceac-9269-441e-951b-a24384d1e1b2).html https://doi.org/10.1016/j.aquatox.2020.105590 https://pure.au.dk/ws/files/220123746/1_s2.0_S0166445X20303404_main.pdf http://www.scopus.com/inward/record.url?scp=85090025252&partnerID=8YFLogxK eng eng https://pure.au.dk/portal/da/publications/proteomics-and-lipidomics-analyses-reveal-modulation-of-lipid-metabolism-by-perfluoroalkyl-substances-in-liver-of-atlantic-cod-gadus-morhua(c31cceac-9269-441e-951b-a24384d1e1b2).html info:eu-repo/semantics/openAccess Dale , K , Yadetie , F , Müller , M B , Pampanin , D M , Gilabert , A , Zhang , X , Tairova , Z , Haarr , A , Lille-Langøy , R , Lyche , J L , Porte , C , Karlsen , O A & Goksøyr , A 2020 , ' Proteomics and lipidomics analyses reveal modulation of lipid metabolism by perfluoroalkyl substances in liver of Atlantic cod ( Gadus morhua ) ' , Aquatic Toxicology , vol. 227 , 105590 . https://doi.org/10.1016/j.aquatox.2020.105590 biomarker Fish mixture toxicity peroxisome proliferator-activated receptor alpha (PPARA) PFAS polycyclic aromatic hydrocarbons (PAH) POLYCYCLIC AROMATIC-HYDROCARBONS PEROXISOME PROLIFERATOR PAH IN-VIVO GENE-EXPRESSION FISH BILE RECEPTORS BIOMARKER RESPONSES PFOS WATER article 2020 ftuniaarhuspubl https://doi.org/10.1016/j.aquatox.2020.105590 2023-11-30T00:00:41Z The aim of the present study was to investigate effects of defined mixtures of polycyclic aromatic hydrocarbons (PAHs) and perfluoroalkyl substances (PFASs), at low, environmentally relevant (1× = L), or high (20× = H) doses, on biological responses in Atlantic cod (Gadus morhua). To this end, farmed juvenile cod were exposed at day 0 and day 7 via intraperitoneal (i.p.) injections, in a two-week in vivo experiment. In total, there were 10 groups of fish (n = 21-22): two control groups, four separate exposure groups of PAH and PFAS mixtures (L, H), and four groups combining PAH and PFAS mixtures (L/L, H/L, L/H, H/H). Body burden analyses confirmed a dose-dependent accumulation of PFASs in cod liver and PAH metabolites in bile. The hepatosomatic index (HSI) was significantly reduced for three of the combined PAH/PFAS exposure groups (L-PAH/H-PFAS, H-PAH/L-PFAS, H-PAH/H-PFAS). Analysis of the hepatic proteome identified that pathways related to lipid degradation were significantly affected by PFAS exposure, including upregulation of enzymes in fatty acid degradation pathways, such as fatty acid β-oxidation. The increased abundances of enzymes in lipid catabolic pathways paralleled with decreasing levels of triacylglycerols (TGs) in the H-PFAS exposure group, suggest that PFAS increase lipid catabolism in Atlantic cod. Markers of oxidative stress, including catalase and glutathione S-transferase activities were also induced by PFAS exposure. Only minor and non-significant differences between exposure groups and control were found for cyp1a and acox1 gene expressions, vitellogenin concentrations in plasma, Cyp1a protein synthesis and DNA fragmentation. In summary, our combined proteomics and lipidomics analyses indicate that PFAS may disrupt lipid homeostasis in Atlantic cod. Article in Journal/Newspaper atlantic cod Gadus morhua Aarhus University: Research Aquatic Toxicology 227 105590 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
biomarker Fish mixture toxicity peroxisome proliferator-activated receptor alpha (PPARA) PFAS polycyclic aromatic hydrocarbons (PAH) POLYCYCLIC AROMATIC-HYDROCARBONS PEROXISOME PROLIFERATOR PAH IN-VIVO GENE-EXPRESSION FISH BILE RECEPTORS BIOMARKER RESPONSES PFOS WATER |
spellingShingle |
biomarker Fish mixture toxicity peroxisome proliferator-activated receptor alpha (PPARA) PFAS polycyclic aromatic hydrocarbons (PAH) POLYCYCLIC AROMATIC-HYDROCARBONS PEROXISOME PROLIFERATOR PAH IN-VIVO GENE-EXPRESSION FISH BILE RECEPTORS BIOMARKER RESPONSES PFOS WATER Dale, Karina Yadetie, Fekadu Müller, Mette Bjørge Pampanin, Daniela M. Gilabert, Alejandra Zhang, Xiaokang Tairova, Zhanna Haarr, Ane Lille-Langøy, Roger Lyche, Jan Ludvig Porte, Cinta Karlsen, Odd André Goksøyr, Anders Proteomics and lipidomics analyses reveal modulation of lipid metabolism by perfluoroalkyl substances in liver of Atlantic cod ( Gadus morhua ) |
topic_facet |
biomarker Fish mixture toxicity peroxisome proliferator-activated receptor alpha (PPARA) PFAS polycyclic aromatic hydrocarbons (PAH) POLYCYCLIC AROMATIC-HYDROCARBONS PEROXISOME PROLIFERATOR PAH IN-VIVO GENE-EXPRESSION FISH BILE RECEPTORS BIOMARKER RESPONSES PFOS WATER |
description |
The aim of the present study was to investigate effects of defined mixtures of polycyclic aromatic hydrocarbons (PAHs) and perfluoroalkyl substances (PFASs), at low, environmentally relevant (1× = L), or high (20× = H) doses, on biological responses in Atlantic cod (Gadus morhua). To this end, farmed juvenile cod were exposed at day 0 and day 7 via intraperitoneal (i.p.) injections, in a two-week in vivo experiment. In total, there were 10 groups of fish (n = 21-22): two control groups, four separate exposure groups of PAH and PFAS mixtures (L, H), and four groups combining PAH and PFAS mixtures (L/L, H/L, L/H, H/H). Body burden analyses confirmed a dose-dependent accumulation of PFASs in cod liver and PAH metabolites in bile. The hepatosomatic index (HSI) was significantly reduced for three of the combined PAH/PFAS exposure groups (L-PAH/H-PFAS, H-PAH/L-PFAS, H-PAH/H-PFAS). Analysis of the hepatic proteome identified that pathways related to lipid degradation were significantly affected by PFAS exposure, including upregulation of enzymes in fatty acid degradation pathways, such as fatty acid β-oxidation. The increased abundances of enzymes in lipid catabolic pathways paralleled with decreasing levels of triacylglycerols (TGs) in the H-PFAS exposure group, suggest that PFAS increase lipid catabolism in Atlantic cod. Markers of oxidative stress, including catalase and glutathione S-transferase activities were also induced by PFAS exposure. Only minor and non-significant differences between exposure groups and control were found for cyp1a and acox1 gene expressions, vitellogenin concentrations in plasma, Cyp1a protein synthesis and DNA fragmentation. In summary, our combined proteomics and lipidomics analyses indicate that PFAS may disrupt lipid homeostasis in Atlantic cod. |
format |
Article in Journal/Newspaper |
author |
Dale, Karina Yadetie, Fekadu Müller, Mette Bjørge Pampanin, Daniela M. Gilabert, Alejandra Zhang, Xiaokang Tairova, Zhanna Haarr, Ane Lille-Langøy, Roger Lyche, Jan Ludvig Porte, Cinta Karlsen, Odd André Goksøyr, Anders |
author_facet |
Dale, Karina Yadetie, Fekadu Müller, Mette Bjørge Pampanin, Daniela M. Gilabert, Alejandra Zhang, Xiaokang Tairova, Zhanna Haarr, Ane Lille-Langøy, Roger Lyche, Jan Ludvig Porte, Cinta Karlsen, Odd André Goksøyr, Anders |
author_sort |
Dale, Karina |
title |
Proteomics and lipidomics analyses reveal modulation of lipid metabolism by perfluoroalkyl substances in liver of Atlantic cod ( Gadus morhua ) |
title_short |
Proteomics and lipidomics analyses reveal modulation of lipid metabolism by perfluoroalkyl substances in liver of Atlantic cod ( Gadus morhua ) |
title_full |
Proteomics and lipidomics analyses reveal modulation of lipid metabolism by perfluoroalkyl substances in liver of Atlantic cod ( Gadus morhua ) |
title_fullStr |
Proteomics and lipidomics analyses reveal modulation of lipid metabolism by perfluoroalkyl substances in liver of Atlantic cod ( Gadus morhua ) |
title_full_unstemmed |
Proteomics and lipidomics analyses reveal modulation of lipid metabolism by perfluoroalkyl substances in liver of Atlantic cod ( Gadus morhua ) |
title_sort |
proteomics and lipidomics analyses reveal modulation of lipid metabolism by perfluoroalkyl substances in liver of atlantic cod ( gadus morhua ) |
publishDate |
2020 |
url |
https://pure.au.dk/portal/da/publications/proteomics-and-lipidomics-analyses-reveal-modulation-of-lipid-metabolism-by-perfluoroalkyl-substances-in-liver-of-atlantic-cod-gadus-morhua(c31cceac-9269-441e-951b-a24384d1e1b2).html https://doi.org/10.1016/j.aquatox.2020.105590 https://pure.au.dk/ws/files/220123746/1_s2.0_S0166445X20303404_main.pdf http://www.scopus.com/inward/record.url?scp=85090025252&partnerID=8YFLogxK |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Dale , K , Yadetie , F , Müller , M B , Pampanin , D M , Gilabert , A , Zhang , X , Tairova , Z , Haarr , A , Lille-Langøy , R , Lyche , J L , Porte , C , Karlsen , O A & Goksøyr , A 2020 , ' Proteomics and lipidomics analyses reveal modulation of lipid metabolism by perfluoroalkyl substances in liver of Atlantic cod ( Gadus morhua ) ' , Aquatic Toxicology , vol. 227 , 105590 . https://doi.org/10.1016/j.aquatox.2020.105590 |
op_relation |
https://pure.au.dk/portal/da/publications/proteomics-and-lipidomics-analyses-reveal-modulation-of-lipid-metabolism-by-perfluoroalkyl-substances-in-liver-of-atlantic-cod-gadus-morhua(c31cceac-9269-441e-951b-a24384d1e1b2).html |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.aquatox.2020.105590 |
container_title |
Aquatic Toxicology |
container_volume |
227 |
container_start_page |
105590 |
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1786199282016059392 |