Methyl mercury (MeHg) in vitro exposure alters mitogen-induced lymphocyte proliferation and cytokine expression in Steller sea lion (Eumetopias jubatus) pups
Steller sea lions (Eumetopias jubatus, SSLs) are managed as two distinct population segments within U.S. waters: the endangered western distinct population segment and the recently delisted eastern distinct population segment. Recent studies reported concentrations of mercury in several tissues coll...
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2020
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Online Access: | https://pure.au.dk/portal/da/publications/methyl-mercury-mehg-in-vitro-exposure-alters-mitogeninduced-lymphocyte-proliferation-and-cytokine-expression-in-steller-sea-lion-eumetopias-jubatus-pups(c20acace-f3b4-4fc7-a404-2cb8e594a91f).html https://doi.org/10.1016/j.scitotenv.2020.138308 http://www.scopus.com/inward/record.url?scp=85083090771&partnerID=8YFLogxK |
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ftuniaarhuspubl:oai:pure.atira.dk:publications/c20acace-f3b4-4fc7-a404-2cb8e594a91f 2023-12-24T10:07:39+01:00 Methyl mercury (MeHg) in vitro exposure alters mitogen-induced lymphocyte proliferation and cytokine expression in Steller sea lion (Eumetopias jubatus) pups Levin, Milton Jasperse, Lindsay Desforges, Jean Pierre O'Hara, Todd Rea, Lorrie Castellini, J. Margaret Maniscalco, John M. Fadely, Brian Keogh, Mandy 2020-07 https://pure.au.dk/portal/da/publications/methyl-mercury-mehg-in-vitro-exposure-alters-mitogeninduced-lymphocyte-proliferation-and-cytokine-expression-in-steller-sea-lion-eumetopias-jubatus-pups(c20acace-f3b4-4fc7-a404-2cb8e594a91f).html https://doi.org/10.1016/j.scitotenv.2020.138308 http://www.scopus.com/inward/record.url?scp=85083090771&partnerID=8YFLogxK eng eng https://pure.au.dk/portal/da/publications/methyl-mercury-mehg-in-vitro-exposure-alters-mitogeninduced-lymphocyte-proliferation-and-cytokine-expression-in-steller-sea-lion-eumetopias-jubatus-pups(c20acace-f3b4-4fc7-a404-2cb8e594a91f).html info:eu-repo/semantics/restrictedAccess Levin , M , Jasperse , L , Desforges , J P , O'Hara , T , Rea , L , Castellini , J M , Maniscalco , J M , Fadely , B & Keogh , M 2020 , ' Methyl mercury (MeHg) in vitro exposure alters mitogen-induced lymphocyte proliferation and cytokine expression in Steller sea lion (Eumetopias jubatus) pups ' , Science of the total Environment , vol. 725 , 138308 . https://doi.org/10.1016/j.scitotenv.2020.138308 Cytokine Immune Lymphocyte proliferation Methyl mercury Steller sea lion article 2020 ftuniaarhuspubl https://doi.org/10.1016/j.scitotenv.2020.138308 2023-11-30T00:00:32Z Steller sea lions (Eumetopias jubatus, SSLs) are managed as two distinct population segments within U.S. waters: the endangered western distinct population segment and the recently delisted eastern distinct population segment. Recent studies reported concentrations of mercury in several tissues collected from young SSLs in the Aleutian Islands that were at or above concentrations found to negatively impact health in other fish-eating mammals. However, there are limited studies which have investigated the range of mercury concentrations that may negatively influence the SSL immune system. This study assessed relationships between methyl mercury (MeHg + ) concentrations and two immune functions, lymphocyte proliferation and cytokine expression. Peripheral blood mononuclear cells (PBMCs) were isolated and cryopreserved from pups on three rookeries within the western distinct population segment: Chiswell Island, Ulak, and Agattu Islands. Lymphocyte proliferation and cytokine expression were assessed in vitro using thawed PBMCs with exposure to MeHg + (unexposed control, 0.001, 0.01, and 0.1 μg/ml). Lymphocyte proliferation was measured without and with stimulation with a T cell mitogen (ConA) and B cell mitogen (LPS) and the concentration of cytokines was measured in the cell culture supernatant (with and without ConA or LPS). Spontaneous lymphocyte proliferation was significantly increased at 0.01 and 0.1 μg/ml. T lymphocyte proliferation was significantly increased at 0.001 μg/ml and 0.1 μg/ml, while B lymphocyte proliferation was decreased at 0.1 μg/ml. Cytokine concentrations for INFγ, IL-10, IL-6, and TNFα were reduced at 0.1 μg/ml upon either T or B cell mitogen stimulation, with the exception for IL-10, where 0.1 μg/ml reduced IL-10 concentration compared to unstimulated cells. These data suggest immune functions were affected by MeHg + exposure requiring in vivo follow up investigations. The observed modulation of immune functions is of concern as any toxicant-induced modulation may adversely affect the ... Article in Journal/Newspaper Agattu Aleutian Islands Aarhus University: Research Science of The Total Environment 725 138308 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
Cytokine Immune Lymphocyte proliferation Methyl mercury Steller sea lion |
spellingShingle |
Cytokine Immune Lymphocyte proliferation Methyl mercury Steller sea lion Levin, Milton Jasperse, Lindsay Desforges, Jean Pierre O'Hara, Todd Rea, Lorrie Castellini, J. Margaret Maniscalco, John M. Fadely, Brian Keogh, Mandy Methyl mercury (MeHg) in vitro exposure alters mitogen-induced lymphocyte proliferation and cytokine expression in Steller sea lion (Eumetopias jubatus) pups |
topic_facet |
Cytokine Immune Lymphocyte proliferation Methyl mercury Steller sea lion |
description |
Steller sea lions (Eumetopias jubatus, SSLs) are managed as two distinct population segments within U.S. waters: the endangered western distinct population segment and the recently delisted eastern distinct population segment. Recent studies reported concentrations of mercury in several tissues collected from young SSLs in the Aleutian Islands that were at or above concentrations found to negatively impact health in other fish-eating mammals. However, there are limited studies which have investigated the range of mercury concentrations that may negatively influence the SSL immune system. This study assessed relationships between methyl mercury (MeHg + ) concentrations and two immune functions, lymphocyte proliferation and cytokine expression. Peripheral blood mononuclear cells (PBMCs) were isolated and cryopreserved from pups on three rookeries within the western distinct population segment: Chiswell Island, Ulak, and Agattu Islands. Lymphocyte proliferation and cytokine expression were assessed in vitro using thawed PBMCs with exposure to MeHg + (unexposed control, 0.001, 0.01, and 0.1 μg/ml). Lymphocyte proliferation was measured without and with stimulation with a T cell mitogen (ConA) and B cell mitogen (LPS) and the concentration of cytokines was measured in the cell culture supernatant (with and without ConA or LPS). Spontaneous lymphocyte proliferation was significantly increased at 0.01 and 0.1 μg/ml. T lymphocyte proliferation was significantly increased at 0.001 μg/ml and 0.1 μg/ml, while B lymphocyte proliferation was decreased at 0.1 μg/ml. Cytokine concentrations for INFγ, IL-10, IL-6, and TNFα were reduced at 0.1 μg/ml upon either T or B cell mitogen stimulation, with the exception for IL-10, where 0.1 μg/ml reduced IL-10 concentration compared to unstimulated cells. These data suggest immune functions were affected by MeHg + exposure requiring in vivo follow up investigations. The observed modulation of immune functions is of concern as any toxicant-induced modulation may adversely affect the ... |
format |
Article in Journal/Newspaper |
author |
Levin, Milton Jasperse, Lindsay Desforges, Jean Pierre O'Hara, Todd Rea, Lorrie Castellini, J. Margaret Maniscalco, John M. Fadely, Brian Keogh, Mandy |
author_facet |
Levin, Milton Jasperse, Lindsay Desforges, Jean Pierre O'Hara, Todd Rea, Lorrie Castellini, J. Margaret Maniscalco, John M. Fadely, Brian Keogh, Mandy |
author_sort |
Levin, Milton |
title |
Methyl mercury (MeHg) in vitro exposure alters mitogen-induced lymphocyte proliferation and cytokine expression in Steller sea lion (Eumetopias jubatus) pups |
title_short |
Methyl mercury (MeHg) in vitro exposure alters mitogen-induced lymphocyte proliferation and cytokine expression in Steller sea lion (Eumetopias jubatus) pups |
title_full |
Methyl mercury (MeHg) in vitro exposure alters mitogen-induced lymphocyte proliferation and cytokine expression in Steller sea lion (Eumetopias jubatus) pups |
title_fullStr |
Methyl mercury (MeHg) in vitro exposure alters mitogen-induced lymphocyte proliferation and cytokine expression in Steller sea lion (Eumetopias jubatus) pups |
title_full_unstemmed |
Methyl mercury (MeHg) in vitro exposure alters mitogen-induced lymphocyte proliferation and cytokine expression in Steller sea lion (Eumetopias jubatus) pups |
title_sort |
methyl mercury (mehg) in vitro exposure alters mitogen-induced lymphocyte proliferation and cytokine expression in steller sea lion (eumetopias jubatus) pups |
publishDate |
2020 |
url |
https://pure.au.dk/portal/da/publications/methyl-mercury-mehg-in-vitro-exposure-alters-mitogeninduced-lymphocyte-proliferation-and-cytokine-expression-in-steller-sea-lion-eumetopias-jubatus-pups(c20acace-f3b4-4fc7-a404-2cb8e594a91f).html https://doi.org/10.1016/j.scitotenv.2020.138308 http://www.scopus.com/inward/record.url?scp=85083090771&partnerID=8YFLogxK |
genre |
Agattu Aleutian Islands |
genre_facet |
Agattu Aleutian Islands |
op_source |
Levin , M , Jasperse , L , Desforges , J P , O'Hara , T , Rea , L , Castellini , J M , Maniscalco , J M , Fadely , B & Keogh , M 2020 , ' Methyl mercury (MeHg) in vitro exposure alters mitogen-induced lymphocyte proliferation and cytokine expression in Steller sea lion (Eumetopias jubatus) pups ' , Science of the total Environment , vol. 725 , 138308 . https://doi.org/10.1016/j.scitotenv.2020.138308 |
op_relation |
https://pure.au.dk/portal/da/publications/methyl-mercury-mehg-in-vitro-exposure-alters-mitogeninduced-lymphocyte-proliferation-and-cytokine-expression-in-steller-sea-lion-eumetopias-jubatus-pups(c20acace-f3b4-4fc7-a404-2cb8e594a91f).html |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.scitotenv.2020.138308 |
container_title |
Science of The Total Environment |
container_volume |
725 |
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138308 |
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1786211007384780800 |