Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius)
Abstract: Old lead–zinc (Pb–Zn) mining sites in Greenland have increased the environmental concentration of Pb in local marine organisms, including the shorthorn sculpin. Organ metal concentrations and histopathology have been used in environmental monitoring programs to evaluate metal exposure and...
Published in: | Ecotoxicology |
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Format: | Article in Journal/Newspaper |
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2022
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Online Access: | https://pure.au.dk/portal/da/publications/effects-of-exposure-to-environmentally-relevant-concentrations-of-lead-pb-on-expression-of-stress-and-immunerelated-genes-and-micrornas-in-shorthorn-sculpins-myoxocephalus-scorpius(c146db35-03bc-4218-a68f-6cb5d5487ab2).html https://doi.org/10.1007/s10646-022-02575-x https://pure.au.dk/ws/files/333400204/s10646_022_02575_x.pdf http://www.scopus.com/inward/record.url?scp=85137253404&partnerID=8YFLogxK |
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ftuniaarhuspubl:oai:pure.atira.dk:publications/c146db35-03bc-4218-a68f-6cb5d5487ab2 2023-08-27T04:07:47+02:00 Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius) Jantawongsri, Khattapan Nørregaard, Rasmus Dyrmose Bach, Lis Dietz, Rune Sonne, Christian Jørgensen, Kasper Lierhagen, Syverin Ciesielski, Tomasz Maciej Jenssen, Bjørn Munro Waugh, Courtney Alice Eriksen, Ruth Nowak, Barbara Anderson, Kelli 2022-09 application/pdf https://pure.au.dk/portal/da/publications/effects-of-exposure-to-environmentally-relevant-concentrations-of-lead-pb-on-expression-of-stress-and-immunerelated-genes-and-micrornas-in-shorthorn-sculpins-myoxocephalus-scorpius(c146db35-03bc-4218-a68f-6cb5d5487ab2).html https://doi.org/10.1007/s10646-022-02575-x https://pure.au.dk/ws/files/333400204/s10646_022_02575_x.pdf http://www.scopus.com/inward/record.url?scp=85137253404&partnerID=8YFLogxK eng eng info:eu-repo/semantics/openAccess Jantawongsri , K , Nørregaard , R D , Bach , L , Dietz , R , Sonne , C , Jørgensen , K , Lierhagen , S , Ciesielski , T M , Jenssen , B M , Waugh , C A , Eriksen , R , Nowak , B & Anderson , K 2022 , ' Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius) ' , Ecotoxicology , vol. 31 , no. 7 , pp. 1068-1077 . https://doi.org/10.1007/s10646-022-02575-x Arctic lead–zinc mines Dissolved Pb exposure Gene expression Greenland sculpin Immune-related gene Metal stress-related gene article 2022 ftuniaarhuspubl https://doi.org/10.1007/s10646-022-02575-x 2023-08-02T22:58:24Z Abstract: Old lead–zinc (Pb–Zn) mining sites in Greenland have increased the environmental concentration of Pb in local marine organisms, including the shorthorn sculpin. Organ metal concentrations and histopathology have been used in environmental monitoring programs to evaluate metal exposure and subsequent effects in shorthorn sculpins. So far, no study has reported the impact of heavy metals on gene expression involved in metal-related stress and immune responses in sculpins. The aim of this study was to investigate the effect of exposure to environmentally relevant waterborne Pb (0.73 ± 0.35 μg/L) on hepatic gene expression of metallothionein (mt), immunoglobulin M (igm), and microRNAs (miRNAs; mir132 and mir155) associated with immune responses in the shorthorn sculpin compared to a control group. The mt and igm expression were upregulated in the Pb-exposed group compared to the control group. The transcripts of mir132 and mir155 were not different in sculpins between the Pb-exposed and control group; however, miRNA levels were significantly correlated with Pb liver concentrations. Furthermore, there was a positive correlation between liver Pb concentrations and igm, and a positive relationship between igm and mir155. The results indicate that exposure to Pb similar to those concentrations reported in in marine waters around Greenland Pb–Zn mine sites influences the mt and immune responses in shorthorn sculpins. This is the first study to identify candidate molecular markers in the shorthorn sculpins exposed to waterborne environmentally relevant Pb suggesting mt and igm as potential molecular markers of exposure to be applied in future assessments of the marine environment near Arctic mining sites. Article in Journal/Newspaper Arctic Greenland Aarhus University: Research Arctic Greenland Ecotoxicology 31 7 1068 1077 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
Arctic lead–zinc mines Dissolved Pb exposure Gene expression Greenland sculpin Immune-related gene Metal stress-related gene |
spellingShingle |
Arctic lead–zinc mines Dissolved Pb exposure Gene expression Greenland sculpin Immune-related gene Metal stress-related gene Jantawongsri, Khattapan Nørregaard, Rasmus Dyrmose Bach, Lis Dietz, Rune Sonne, Christian Jørgensen, Kasper Lierhagen, Syverin Ciesielski, Tomasz Maciej Jenssen, Bjørn Munro Waugh, Courtney Alice Eriksen, Ruth Nowak, Barbara Anderson, Kelli Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius) |
topic_facet |
Arctic lead–zinc mines Dissolved Pb exposure Gene expression Greenland sculpin Immune-related gene Metal stress-related gene |
description |
Abstract: Old lead–zinc (Pb–Zn) mining sites in Greenland have increased the environmental concentration of Pb in local marine organisms, including the shorthorn sculpin. Organ metal concentrations and histopathology have been used in environmental monitoring programs to evaluate metal exposure and subsequent effects in shorthorn sculpins. So far, no study has reported the impact of heavy metals on gene expression involved in metal-related stress and immune responses in sculpins. The aim of this study was to investigate the effect of exposure to environmentally relevant waterborne Pb (0.73 ± 0.35 μg/L) on hepatic gene expression of metallothionein (mt), immunoglobulin M (igm), and microRNAs (miRNAs; mir132 and mir155) associated with immune responses in the shorthorn sculpin compared to a control group. The mt and igm expression were upregulated in the Pb-exposed group compared to the control group. The transcripts of mir132 and mir155 were not different in sculpins between the Pb-exposed and control group; however, miRNA levels were significantly correlated with Pb liver concentrations. Furthermore, there was a positive correlation between liver Pb concentrations and igm, and a positive relationship between igm and mir155. The results indicate that exposure to Pb similar to those concentrations reported in in marine waters around Greenland Pb–Zn mine sites influences the mt and immune responses in shorthorn sculpins. This is the first study to identify candidate molecular markers in the shorthorn sculpins exposed to waterborne environmentally relevant Pb suggesting mt and igm as potential molecular markers of exposure to be applied in future assessments of the marine environment near Arctic mining sites. |
format |
Article in Journal/Newspaper |
author |
Jantawongsri, Khattapan Nørregaard, Rasmus Dyrmose Bach, Lis Dietz, Rune Sonne, Christian Jørgensen, Kasper Lierhagen, Syverin Ciesielski, Tomasz Maciej Jenssen, Bjørn Munro Waugh, Courtney Alice Eriksen, Ruth Nowak, Barbara Anderson, Kelli |
author_facet |
Jantawongsri, Khattapan Nørregaard, Rasmus Dyrmose Bach, Lis Dietz, Rune Sonne, Christian Jørgensen, Kasper Lierhagen, Syverin Ciesielski, Tomasz Maciej Jenssen, Bjørn Munro Waugh, Courtney Alice Eriksen, Ruth Nowak, Barbara Anderson, Kelli |
author_sort |
Jantawongsri, Khattapan |
title |
Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius) |
title_short |
Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius) |
title_full |
Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius) |
title_fullStr |
Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius) |
title_full_unstemmed |
Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius) |
title_sort |
effects of exposure to environmentally relevant concentrations of lead (pb) on expression of stress and immune-related genes, and micrornas in shorthorn sculpins (myoxocephalus scorpius) |
publishDate |
2022 |
url |
https://pure.au.dk/portal/da/publications/effects-of-exposure-to-environmentally-relevant-concentrations-of-lead-pb-on-expression-of-stress-and-immunerelated-genes-and-micrornas-in-shorthorn-sculpins-myoxocephalus-scorpius(c146db35-03bc-4218-a68f-6cb5d5487ab2).html https://doi.org/10.1007/s10646-022-02575-x https://pure.au.dk/ws/files/333400204/s10646_022_02575_x.pdf http://www.scopus.com/inward/record.url?scp=85137253404&partnerID=8YFLogxK |
geographic |
Arctic Greenland |
geographic_facet |
Arctic Greenland |
genre |
Arctic Greenland |
genre_facet |
Arctic Greenland |
op_source |
Jantawongsri , K , Nørregaard , R D , Bach , L , Dietz , R , Sonne , C , Jørgensen , K , Lierhagen , S , Ciesielski , T M , Jenssen , B M , Waugh , C A , Eriksen , R , Nowak , B & Anderson , K 2022 , ' Effects of exposure to environmentally relevant concentrations of lead (Pb) on expression of stress and immune-related genes, and microRNAs in shorthorn sculpins (Myoxocephalus scorpius) ' , Ecotoxicology , vol. 31 , no. 7 , pp. 1068-1077 . https://doi.org/10.1007/s10646-022-02575-x |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1007/s10646-022-02575-x |
container_title |
Ecotoxicology |
container_volume |
31 |
container_issue |
7 |
container_start_page |
1068 |
op_container_end_page |
1077 |
_version_ |
1775348501447704576 |