Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience
Increases in Arctic temperatures have accelerated melting of the Greenland icesheet, exposing intertidal organisms, such as the blue mussel Mytilus edulis , to high air temperatures and low salinities in summer. However, the interaction of these combined stressors is poorly described at the transcri...
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Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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2022
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Online Access: | https://pure.au.dk/portal/en/publications/bd3551a3-fae0-4f2d-aa66-758436f13fa2 https://doi.org/10.3390/genes13010155 https://pure.au.dk/ws/files/230132930/genes_13_00155_v2.pdf http://www.scopus.com/inward/record.url?scp=85122981305&partnerID=8YFLogxK |
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ftuniaarhuspubl:oai:pure.atira.dk:publications/bd3551a3-fae0-4f2d-aa66-758436f13fa2 2024-09-09T19:15:17+00:00 Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience Barrett, Nicholas Thyrring, Jakob Harper , Elizabeth M. Sejr, Mikael Kristian Sørensen, Jesper Givskov Peck, Lloyd Clark, Melody S. 2022-01 application/pdf https://pure.au.dk/portal/en/publications/bd3551a3-fae0-4f2d-aa66-758436f13fa2 https://doi.org/10.3390/genes13010155 https://pure.au.dk/ws/files/230132930/genes_13_00155_v2.pdf http://www.scopus.com/inward/record.url?scp=85122981305&partnerID=8YFLogxK eng eng https://pure.au.dk/portal/en/publications/bd3551a3-fae0-4f2d-aa66-758436f13fa2 info:eu-repo/semantics/openAccess Barrett , N , Thyrring , J , Harper , E M , Sejr , M K , Sørensen , J G , Peck , L & Clark , M S 2022 , ' Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience ' , Genes , vol. 13 , no. 1 , 155 . https://doi.org/10.3390/genes13010155 Acclimation Aquaporines Blue mussel Cellular stress response Climate change Freshening Salinity Thermal tolerance Transcriptome article 2022 ftuniaarhuspubl https://doi.org/10.3390/genes13010155 2024-06-18T14:24:25Z Increases in Arctic temperatures have accelerated melting of the Greenland icesheet, exposing intertidal organisms, such as the blue mussel Mytilus edulis , to high air temperatures and low salinities in summer. However, the interaction of these combined stressors is poorly described at the transcriptional level. Comparing expression profiles of M. edulis from experimentally warmed (30 °C and 33 °C) animals kept at control (23‰) and low salinities (15‰) revealed a significant lack of enrichment for Gene Ontology terms (GO), indicating that similar processes were active under all conditions. However, there was a progressive increase in the abundance of upregulated genes as each stressor was applied, with synergistic increases at 33 °C and 15‰, suggesting combined stressors push the animal towards their tolerance thresholds. Further analyses comparing the effects of salinity alone (23‰, 15‰ and 5‰) showed high expression of stress and osmoregulatory marker genes at the lowest salinity, implying that the cell is carrying out intracellular osmoregulation to maintain the cytosol as hyperosmotic. Identification of aquaporins and vacuolar-type ATPase transcripts suggested the cell may use fluid-filled cavities to excrete excess intracellular water, as previously identified in embryonic freshwater mussels. These results indicate that M. edulis has considerable resilience to heat stress and highly efficient mechanisms to acclimatise to lowered salinity in a changing world Article in Journal/Newspaper Arctic Arctic Climate change Greenland Aarhus University: Research Arctic Greenland Genes 13 1 155 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
Acclimation Aquaporines Blue mussel Cellular stress response Climate change Freshening Salinity Thermal tolerance Transcriptome |
spellingShingle |
Acclimation Aquaporines Blue mussel Cellular stress response Climate change Freshening Salinity Thermal tolerance Transcriptome Barrett, Nicholas Thyrring, Jakob Harper , Elizabeth M. Sejr, Mikael Kristian Sørensen, Jesper Givskov Peck, Lloyd Clark, Melody S. Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience |
topic_facet |
Acclimation Aquaporines Blue mussel Cellular stress response Climate change Freshening Salinity Thermal tolerance Transcriptome |
description |
Increases in Arctic temperatures have accelerated melting of the Greenland icesheet, exposing intertidal organisms, such as the blue mussel Mytilus edulis , to high air temperatures and low salinities in summer. However, the interaction of these combined stressors is poorly described at the transcriptional level. Comparing expression profiles of M. edulis from experimentally warmed (30 °C and 33 °C) animals kept at control (23‰) and low salinities (15‰) revealed a significant lack of enrichment for Gene Ontology terms (GO), indicating that similar processes were active under all conditions. However, there was a progressive increase in the abundance of upregulated genes as each stressor was applied, with synergistic increases at 33 °C and 15‰, suggesting combined stressors push the animal towards their tolerance thresholds. Further analyses comparing the effects of salinity alone (23‰, 15‰ and 5‰) showed high expression of stress and osmoregulatory marker genes at the lowest salinity, implying that the cell is carrying out intracellular osmoregulation to maintain the cytosol as hyperosmotic. Identification of aquaporins and vacuolar-type ATPase transcripts suggested the cell may use fluid-filled cavities to excrete excess intracellular water, as previously identified in embryonic freshwater mussels. These results indicate that M. edulis has considerable resilience to heat stress and highly efficient mechanisms to acclimatise to lowered salinity in a changing world |
format |
Article in Journal/Newspaper |
author |
Barrett, Nicholas Thyrring, Jakob Harper , Elizabeth M. Sejr, Mikael Kristian Sørensen, Jesper Givskov Peck, Lloyd Clark, Melody S. |
author_facet |
Barrett, Nicholas Thyrring, Jakob Harper , Elizabeth M. Sejr, Mikael Kristian Sørensen, Jesper Givskov Peck, Lloyd Clark, Melody S. |
author_sort |
Barrett, Nicholas |
title |
Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience |
title_short |
Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience |
title_full |
Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience |
title_fullStr |
Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience |
title_full_unstemmed |
Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience |
title_sort |
molecular responses to thermal and osmotic stress in arctic intertidal mussels ( mytilus edulis ): the limits of resilience |
publishDate |
2022 |
url |
https://pure.au.dk/portal/en/publications/bd3551a3-fae0-4f2d-aa66-758436f13fa2 https://doi.org/10.3390/genes13010155 https://pure.au.dk/ws/files/230132930/genes_13_00155_v2.pdf http://www.scopus.com/inward/record.url?scp=85122981305&partnerID=8YFLogxK |
geographic |
Arctic Greenland |
geographic_facet |
Arctic Greenland |
genre |
Arctic Arctic Climate change Greenland |
genre_facet |
Arctic Arctic Climate change Greenland |
op_source |
Barrett , N , Thyrring , J , Harper , E M , Sejr , M K , Sørensen , J G , Peck , L & Clark , M S 2022 , ' Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience ' , Genes , vol. 13 , no. 1 , 155 . https://doi.org/10.3390/genes13010155 |
op_relation |
https://pure.au.dk/portal/en/publications/bd3551a3-fae0-4f2d-aa66-758436f13fa2 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/genes13010155 |
container_title |
Genes |
container_volume |
13 |
container_issue |
1 |
container_start_page |
155 |
_version_ |
1809755376053125120 |