DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica

We assessed fungal diversity present in air and freshly deposited snow samples obtained from Livingston Island, Antarctica, using DNA metabarcoding through high throughput sequencing (HTS). A total of 740 m3 of air were pumped through a 0.22 µm membrane. Snow obtained shortly after deposition was ke...

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Published in:Scientific Reports
Main Authors: Rosa, Luiz Henrique, Pinto, Otávio Henrique Bezerra, Šantl-Temkiv, Tina, Convey, Peter, Carvalho-Silva, Micheline, Rosa, Carlos Augusto, Câmara, Paulo E A S
Format: Article in Journal/Newspaper
Language:English
Published: 2020
Subjects:
Online Access:https://pure.au.dk/portal/en/publications/b7a8e80a-d453-46cf-8d97-8f598a99e3d1
https://doi.org/10.1038/s41598-020-78630-6
http://www.scopus.com/inward/record.url?scp=85095595523&partnerID=8YFLogxK
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author Rosa, Luiz Henrique
Pinto, Otávio Henrique Bezerra
Šantl-Temkiv, Tina
Convey, Peter
Carvalho-Silva, Micheline
Rosa, Carlos Augusto
Câmara, Paulo E A S
author_facet Rosa, Luiz Henrique
Pinto, Otávio Henrique Bezerra
Šantl-Temkiv, Tina
Convey, Peter
Carvalho-Silva, Micheline
Rosa, Carlos Augusto
Câmara, Paulo E A S
author_sort Rosa, Luiz Henrique
collection Aarhus University: Research
container_issue 1
container_title Scientific Reports
container_volume 10
description We assessed fungal diversity present in air and freshly deposited snow samples obtained from Livingston Island, Antarctica, using DNA metabarcoding through high throughput sequencing (HTS). A total of 740 m3 of air were pumped through a 0.22 µm membrane. Snow obtained shortly after deposition was kept at room temperature and yielded 3.760 L of water, which was filtered using Sterivex membranes of 0.22 µm mesh size. The total DNA present was extracted and sequenced. We detected 171 fungal amplicon sequence variants (ASVs), 70 from the air and 142 from the snow. They were dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota and Mucoromycota. Pseudogymnoascus, Cladosporium, Mortierella and Penicillium sp. were the most dominant ASVs detected in the air in rank order. In snow, Cladosporium, Pseudogymnoascus, Penicillium, Meyerozyma, Lecidea, Malassezia, Hanseniaspora, Austroplaca, Mortierella, Rhodotorula, Penicillium, Thelebolus, Aspergillus, Poaceicola, Glarea and Lecanora were the dominant ASVs present. In general, the two fungal assemblages displayed high diversity, richness, and dominance indices, with the assemblage found in snow having the highest diversity indices. Of the total fungal ASVs detected, 29 were only present in the air sample and 101 in the snow sample, with only 41 present in both samples; however, when only the dominant taxa from both samples were compared none occurred only in the air and, among the rare portion, 26 taxa occurred in both air and snow. Application of HTS revealed the presence of a more diverse fungal community in the air and snow of Livingston Island in comparison with studies using traditional isolation methods. The assemblages were dominated by cold-adapted and cosmopolitan fungal taxa, including members of the genera Pseudogymnoascus, Malassezia and Rhodotorula, which include some taxa reported as opportunistic. Our results support the hypothesis that the presence of microbiota in the airspora indicates the possibility of dispersal around Antarctica in the air ...
format Article in Journal/Newspaper
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Antarctica
Livingston Island
South Shetland Islands
genre_facet Antarc*
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geographic Livingston Island
South Shetland Islands
geographic_facet Livingston Island
South Shetland Islands
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long_lat ENVELOPE(-60.500,-60.500,-62.600,-62.600)
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op_doi https://doi.org/10.1038/s41598-020-78630-6
op_rights info:eu-repo/semantics/openAccess
op_source Rosa , L H , Pinto , O H B , Šantl-Temkiv , T , Convey , P , Carvalho-Silva , M , Rosa , C A & Câmara , P E A S 2020 , ' DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica ' , Scientific Reports , vol. 10 , no. 1 , 21793 . https://doi.org/10.1038/s41598-020-78630-6
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spelling ftuniaarhuspubl:oai:pure.atira.dk:publications/b7a8e80a-d453-46cf-8d97-8f598a99e3d1 2025-03-02T15:14:11+00:00 DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica Rosa, Luiz Henrique Pinto, Otávio Henrique Bezerra Šantl-Temkiv, Tina Convey, Peter Carvalho-Silva, Micheline Rosa, Carlos Augusto Câmara, Paulo E A S 2020-12 https://pure.au.dk/portal/en/publications/b7a8e80a-d453-46cf-8d97-8f598a99e3d1 https://doi.org/10.1038/s41598-020-78630-6 http://www.scopus.com/inward/record.url?scp=85095595523&partnerID=8YFLogxK eng eng info:eu-repo/semantics/openAccess Rosa , L H , Pinto , O H B , Šantl-Temkiv , T , Convey , P , Carvalho-Silva , M , Rosa , C A & Câmara , P E A S 2020 , ' DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica ' , Scientific Reports , vol. 10 , no. 1 , 21793 . https://doi.org/10.1038/s41598-020-78630-6 article 2020 ftuniaarhuspubl https://doi.org/10.1038/s41598-020-78630-6 2025-02-10T03:54:40Z We assessed fungal diversity present in air and freshly deposited snow samples obtained from Livingston Island, Antarctica, using DNA metabarcoding through high throughput sequencing (HTS). A total of 740 m3 of air were pumped through a 0.22 µm membrane. Snow obtained shortly after deposition was kept at room temperature and yielded 3.760 L of water, which was filtered using Sterivex membranes of 0.22 µm mesh size. The total DNA present was extracted and sequenced. We detected 171 fungal amplicon sequence variants (ASVs), 70 from the air and 142 from the snow. They were dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota and Mucoromycota. Pseudogymnoascus, Cladosporium, Mortierella and Penicillium sp. were the most dominant ASVs detected in the air in rank order. In snow, Cladosporium, Pseudogymnoascus, Penicillium, Meyerozyma, Lecidea, Malassezia, Hanseniaspora, Austroplaca, Mortierella, Rhodotorula, Penicillium, Thelebolus, Aspergillus, Poaceicola, Glarea and Lecanora were the dominant ASVs present. In general, the two fungal assemblages displayed high diversity, richness, and dominance indices, with the assemblage found in snow having the highest diversity indices. Of the total fungal ASVs detected, 29 were only present in the air sample and 101 in the snow sample, with only 41 present in both samples; however, when only the dominant taxa from both samples were compared none occurred only in the air and, among the rare portion, 26 taxa occurred in both air and snow. Application of HTS revealed the presence of a more diverse fungal community in the air and snow of Livingston Island in comparison with studies using traditional isolation methods. The assemblages were dominated by cold-adapted and cosmopolitan fungal taxa, including members of the genera Pseudogymnoascus, Malassezia and Rhodotorula, which include some taxa reported as opportunistic. Our results support the hypothesis that the presence of microbiota in the airspora indicates the possibility of dispersal around Antarctica in the air ... Article in Journal/Newspaper Antarc* Antarctica Livingston Island South Shetland Islands Aarhus University: Research Livingston Island ENVELOPE(-60.500,-60.500,-62.600,-62.600) South Shetland Islands Scientific Reports 10 1
spellingShingle Rosa, Luiz Henrique
Pinto, Otávio Henrique Bezerra
Šantl-Temkiv, Tina
Convey, Peter
Carvalho-Silva, Micheline
Rosa, Carlos Augusto
Câmara, Paulo E A S
DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica
title DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica
title_full DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica
title_fullStr DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica
title_full_unstemmed DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica
title_short DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica
title_sort dna metabarcoding of fungal diversity in air and snow of livingston island, south shetland islands, antarctica
url https://pure.au.dk/portal/en/publications/b7a8e80a-d453-46cf-8d97-8f598a99e3d1
https://doi.org/10.1038/s41598-020-78630-6
http://www.scopus.com/inward/record.url?scp=85095595523&partnerID=8YFLogxK