Spirosoma spitsbergense sp. nov. and Spirosoma luteum sp. nov., isolated from a high Arctic permafrost soil, and emended description of the genus Spirosoma .

• Published in: INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
• Main Authors: Finster, Kai, Herbert, Rodney Andrew, Lomstein, Bente Aagaard
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2009
• Subjects: Adventdalen; Arctic; Norway; Northern Norway; permafrost; Spitsbergen
• Online Access: https://pure.au.dk/portal/en/publications/b3b174c0-3afa-11de-8dc9-000ea68e967b; https://doi.org/10.1099/ijs.0.002725-0

Udgivelsesdato: 2009 Two pigmented, Gram-negative, non-motile, pleomorphic rod-shaped bacteria (strains SPM-9 T and SPM-10 T ) were isolated from a permafrost soil collected from the Adventdalen valley, Spitsbergen, northern Norway. A third isolate (strain M5-H2) was recovered from the same soil sample after the sample had been exposed to simulated Martian environmental conditions. The three strains were characterized taxonomically by using a polyphasic approach. Phylogenetic, chemotaxonomic, physiological and morphological analyses demonstrated that the three isolates were most closely related to members of the genus Spirosoma . 16S rRNA gene sequence data indicated that the three isolates could be divided into two clusters: (i) strain SPM-9 T and (ii) strains SPM-10 T and M5-H2. This grouping was confirmed by DNA-DNA hybridization experiments. Strains SPM-9 T and SPM-10 T exhibited 92 % 16S rRNA gene sequence similarity to both Spirosoma linguale LMG 10896 T and Spirosoma rigui WPCB 118 T . The major fatty acids present in all three isolates were summed feature 3 (comprising iso-C 15:0 2-OH and/or C 16 : 1 7 c 43.0-48.2 % of the total), C 16 : 1 5 c (19.1-21.3 %), C 16 : 0 (6.7-7.3 %), iso-C 17 : 0 3-OH (4.7-6.0 %) and iso-C 15 : 0 (2.6-5.7 %). On the basis of their phenotypic and genotypic characteristics, the new strains are assigned to two novel species of the genus Spirosoma , for which the names Spirosoma spitsbergense sp. nov. and Spirosoma luteum sp. nov. are proposed. The type strain of Spirosoma spitsbergense is SPM-9 T (=NCIMB 14407 T =DSM 19989 T ) and the type strain of Spirosoma luteum is SPM-10 T (=NCIMB 14406 T =DSM 19990 T ). An emended description of the genus Spirosoma is also proposed.