Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry:comparison with two immunoassays and application for longitudinal monitoring in zoos
Analysis of hair cortisol concentrations (HCCs) is a promising method for monitoring long-term stress in mammals. However, previous measurements of HCCs in polar bears (Ursus maritimus) have yielded highly variable results, which are likely due to different methodological approaches. In this study,...
Published in: | General and Comparative Endocrinology |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2021
|
Subjects: | |
Online Access: | https://pure.au.dk/portal/da/publications/analysis-of-hair-steroid-hormones-in-polar-bears-ursus-maritimus-via-liquid-chromatographytandem-mass-spectrometry(a1be359a-d617-4cab-a136-9d8b8401dfe3).html https://doi.org/10.1016/j.ygcen.2021.113837 https://pure.au.dk/ws/files/279519180/1_s2.0_S0016648021001301_main.pdf http://www.scopus.com/inward/record.url?scp=85109434251&partnerID=8YFLogxK |
id |
ftuniaarhuspubl:oai:pure.atira.dk:publications/a1be359a-d617-4cab-a136-9d8b8401dfe3 |
---|---|
record_format |
openpolar |
spelling |
ftuniaarhuspubl:oai:pure.atira.dk:publications/a1be359a-d617-4cab-a136-9d8b8401dfe3 2023-05-15T18:42:24+02:00 Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry:comparison with two immunoassays and application for longitudinal monitoring in zoos Hein, Anna Baumgartner, Katrin von Fersen, Lorenzo Bechshoft, Thea Woelfing, Benno Kirschbaum, Clemens Mastromonaco, Gabriela Greenwood, Alex D. Siebert, Ursula 2021-09 application/pdf https://pure.au.dk/portal/da/publications/analysis-of-hair-steroid-hormones-in-polar-bears-ursus-maritimus-via-liquid-chromatographytandem-mass-spectrometry(a1be359a-d617-4cab-a136-9d8b8401dfe3).html https://doi.org/10.1016/j.ygcen.2021.113837 https://pure.au.dk/ws/files/279519180/1_s2.0_S0016648021001301_main.pdf http://www.scopus.com/inward/record.url?scp=85109434251&partnerID=8YFLogxK eng eng info:eu-repo/semantics/openAccess Hein , A , Baumgartner , K , von Fersen , L , Bechshoft , T , Woelfing , B , Kirschbaum , C , Mastromonaco , G , Greenwood , A D & Siebert , U 2021 , ' Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry : comparison with two immunoassays and application for longitudinal monitoring in zoos ' , General and Comparative Endocrinology , vol. 310 , 113837 . https://doi.org/10.1016/j.ygcen.2021.113837 Cortisol Hair LC–MS/MS Non-invasive Steroid hormones Ursus maritimus article 2021 ftuniaarhuspubl https://doi.org/10.1016/j.ygcen.2021.113837 2022-08-31T22:52:39Z Analysis of hair cortisol concentrations (HCCs) is a promising method for monitoring long-term stress in mammals. However, previous measurements of HCCs in polar bears (Ursus maritimus) have yielded highly variable results, which are likely due to different methodological approaches. In this study, hair samples of zoo-housed polar bears were analyzed for cortisol with two independent immunoassays [an enzyme-linked immunoassay (EIA) and a chemiluminescence assay (CLIA)] and liquid chromatography–tandem mass spectrometry (LC–MS/MS). HCC measurements depended significantly on assay type applied, sample processing (cutting vs. powdering hair) and their interaction. Best agreement was observed between LC–MS/MS and CLIA (R 2 = 0.81 for powdered hair) and sample processing had a minor, albeit significant, effect on obtained HCC measurements in these assays (R 2 > 0.9). EIA measurements were consistently higher than with the other assays. HCC measurement was validated biologically for CLIA and LC–MS/MS in one male polar bear that experienced considerable stress for a prolonged period of time (> 18 weeks). Subsequently, by using the validated LC–MS/MS the measurement of cortisol could be complemented by the analysis of other steroids including cortisone, testosterone and progesterone levels from hair samples collected over a 9-month period (5–13 months) from six zoo-housed polar bears (five males, one female). No seasonal steroid variation was observed except in male progesterone levels. For all steroids except cortisone, a strong body region effect (neck or paw) was observed. Cortisol and cortisone, as well as progesterone and testosterone, concentrations were positively correlated. We show that hair steroid concentrations can be used to longitudinally measure stress and reproductive hormone axes in polar bears. The data established herein provide important basic information regarding methodology and study design for assessing hair steroid hormones. Article in Journal/Newspaper Ursus maritimus Aarhus University: Research Eia ENVELOPE(7.755,7.755,63.024,63.024) General and Comparative Endocrinology 310 113837 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
Cortisol Hair LC–MS/MS Non-invasive Steroid hormones Ursus maritimus |
spellingShingle |
Cortisol Hair LC–MS/MS Non-invasive Steroid hormones Ursus maritimus Hein, Anna Baumgartner, Katrin von Fersen, Lorenzo Bechshoft, Thea Woelfing, Benno Kirschbaum, Clemens Mastromonaco, Gabriela Greenwood, Alex D. Siebert, Ursula Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry:comparison with two immunoassays and application for longitudinal monitoring in zoos |
topic_facet |
Cortisol Hair LC–MS/MS Non-invasive Steroid hormones Ursus maritimus |
description |
Analysis of hair cortisol concentrations (HCCs) is a promising method for monitoring long-term stress in mammals. However, previous measurements of HCCs in polar bears (Ursus maritimus) have yielded highly variable results, which are likely due to different methodological approaches. In this study, hair samples of zoo-housed polar bears were analyzed for cortisol with two independent immunoassays [an enzyme-linked immunoassay (EIA) and a chemiluminescence assay (CLIA)] and liquid chromatography–tandem mass spectrometry (LC–MS/MS). HCC measurements depended significantly on assay type applied, sample processing (cutting vs. powdering hair) and their interaction. Best agreement was observed between LC–MS/MS and CLIA (R 2 = 0.81 for powdered hair) and sample processing had a minor, albeit significant, effect on obtained HCC measurements in these assays (R 2 > 0.9). EIA measurements were consistently higher than with the other assays. HCC measurement was validated biologically for CLIA and LC–MS/MS in one male polar bear that experienced considerable stress for a prolonged period of time (> 18 weeks). Subsequently, by using the validated LC–MS/MS the measurement of cortisol could be complemented by the analysis of other steroids including cortisone, testosterone and progesterone levels from hair samples collected over a 9-month period (5–13 months) from six zoo-housed polar bears (five males, one female). No seasonal steroid variation was observed except in male progesterone levels. For all steroids except cortisone, a strong body region effect (neck or paw) was observed. Cortisol and cortisone, as well as progesterone and testosterone, concentrations were positively correlated. We show that hair steroid concentrations can be used to longitudinally measure stress and reproductive hormone axes in polar bears. The data established herein provide important basic information regarding methodology and study design for assessing hair steroid hormones. |
format |
Article in Journal/Newspaper |
author |
Hein, Anna Baumgartner, Katrin von Fersen, Lorenzo Bechshoft, Thea Woelfing, Benno Kirschbaum, Clemens Mastromonaco, Gabriela Greenwood, Alex D. Siebert, Ursula |
author_facet |
Hein, Anna Baumgartner, Katrin von Fersen, Lorenzo Bechshoft, Thea Woelfing, Benno Kirschbaum, Clemens Mastromonaco, Gabriela Greenwood, Alex D. Siebert, Ursula |
author_sort |
Hein, Anna |
title |
Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry:comparison with two immunoassays and application for longitudinal monitoring in zoos |
title_short |
Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry:comparison with two immunoassays and application for longitudinal monitoring in zoos |
title_full |
Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry:comparison with two immunoassays and application for longitudinal monitoring in zoos |
title_fullStr |
Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry:comparison with two immunoassays and application for longitudinal monitoring in zoos |
title_full_unstemmed |
Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry:comparison with two immunoassays and application for longitudinal monitoring in zoos |
title_sort |
analysis of hair steroid hormones in polar bears (ursus maritimus) via liquid chromatography–tandem mass spectrometry:comparison with two immunoassays and application for longitudinal monitoring in zoos |
publishDate |
2021 |
url |
https://pure.au.dk/portal/da/publications/analysis-of-hair-steroid-hormones-in-polar-bears-ursus-maritimus-via-liquid-chromatographytandem-mass-spectrometry(a1be359a-d617-4cab-a136-9d8b8401dfe3).html https://doi.org/10.1016/j.ygcen.2021.113837 https://pure.au.dk/ws/files/279519180/1_s2.0_S0016648021001301_main.pdf http://www.scopus.com/inward/record.url?scp=85109434251&partnerID=8YFLogxK |
long_lat |
ENVELOPE(7.755,7.755,63.024,63.024) |
geographic |
Eia |
geographic_facet |
Eia |
genre |
Ursus maritimus |
genre_facet |
Ursus maritimus |
op_source |
Hein , A , Baumgartner , K , von Fersen , L , Bechshoft , T , Woelfing , B , Kirschbaum , C , Mastromonaco , G , Greenwood , A D & Siebert , U 2021 , ' Analysis of hair steroid hormones in polar bears (Ursus maritimus) via liquid chromatography–tandem mass spectrometry : comparison with two immunoassays and application for longitudinal monitoring in zoos ' , General and Comparative Endocrinology , vol. 310 , 113837 . https://doi.org/10.1016/j.ygcen.2021.113837 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.ygcen.2021.113837 |
container_title |
General and Comparative Endocrinology |
container_volume |
310 |
container_start_page |
113837 |
_version_ |
1766232058241220608 |