Potential Rhodopsin- and Bacteriochlorophyll-Based Dual Phototrophy in a High Arctic Glacier
Conserving additional energy from sunlight through bacteriochlorophyll (BChl)-based reaction center or proton-pumping rhodopsin is a highly successful life strategy in environmental bacteria. BChl and rhodopsin-based systems display contrasting characteristics in the size of coding operon, cost of b...
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2020
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ftuniaarhuspubl:oai:pure.atira.dk:publications/9b4adc0f-7a32-46b1-af0f-33e37ed2d7fa 2023-12-10T09:43:42+01:00 Potential Rhodopsin- and Bacteriochlorophyll-Based Dual Phototrophy in a High Arctic Glacier Zeng, Yonghui Chen, Xihan Madsen, Anne Mette Zervas, Athanasios Nielsen, Tue Kjærgaard Andrei, Adrian Stefan Lund-Hansen, Lars Chresten Liu, Yongqin Hansen, Lars Hestbjerg 2020-11 https://pure.au.dk/portal/da/publications/potential-rhodopsin-and-bacteriochlorophyllbased-dual-phototrophy-in-a-high-arctic-glacier(9b4adc0f-7a32-46b1-af0f-33e37ed2d7fa).html https://doi.org/10.1128/mBio.02641-20 http://www.scopus.com/inward/record.url?scp=85096815869&partnerID=8YFLogxK eng eng https://pure.au.dk/portal/da/publications/potential-rhodopsin-and-bacteriochlorophyllbased-dual-phototrophy-in-a-high-arctic-glacier(9b4adc0f-7a32-46b1-af0f-33e37ed2d7fa).html info:eu-repo/semantics/openAccess Zeng , Y , Chen , X , Madsen , A M , Zervas , A , Nielsen , T K , Andrei , A S , Lund-Hansen , L C , Liu , Y & Hansen , L H 2020 , ' Potential Rhodopsin- and Bacteriochlorophyll-Based Dual Phototrophy in a High Arctic Glacier ' , mBio , vol. 11 , no. 6 , e02641-20 , pp. 1-8 . https://doi.org/10.1128/mBio.02641-20 bacteriochlorophyll genome evolution glacial bacteria phototrophy rhodopsin article 2020 ftuniaarhuspubl https://doi.org/10.1128/mBio.02641-20 2023-11-16T00:00:23Z Conserving additional energy from sunlight through bacteriochlorophyll (BChl)-based reaction center or proton-pumping rhodopsin is a highly successful life strategy in environmental bacteria. BChl and rhodopsin-based systems display contrasting characteristics in the size of coding operon, cost of biosynthesis, ease of expression control, and efficiency of energy production. This raises an intriguing question of whether a single bacterium has evolved the ability to perform these two types of phototrophy complementarily according to energy needs and environmental conditions. Here, we report four Tardiphaga sp. strains (Alphaproteobacteria) of monophyletic origin isolated from a high Arctic glacier in northeast Greenland (81.566° N, 16.363° W) that are at different evolutionary stages concerning phototrophy. Their >99.8% identical genomes contain footprints of horizontal operon transfer (HOT) of the complete gene clusters encoding BChl- and xanthorhodopsin (XR)-based dual phototrophy. Two strains possess only a complete XR operon, while the other two strains have both a photosynthesis gene cluster and an XR operon in their genomes. All XR operons are heavily surrounded by mobile genetic elements and are located close to a tRNA gene, strongly signaling that a HOT event of the XR operon has occurred recently. Mining public genome databases and our high Arctic glacial and soil metagenomes revealed that phylogenetically diverse bacteria have the metabolic potential of performing BChl- and rhodopsin-based dual phototrophy. Our data provide new insights on how bacteria cope with the harsh and energy-deficient environment in surface glacier, possibly by maximizing the capability of exploiting solar energy.IMPORTANCE Over the course of evolution for billions of years, bacteria that are capable of light-driven energy production have occupied every corner of surface Earth where sunlight can reach. Only two general biological systems have evolved in bacteria to be capable of net energy conservation via light harvesting: ... Article in Journal/Newspaper Arctic Arctic glacier Greenland Aarhus University: Research Arctic Greenland mBio 11 6 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
bacteriochlorophyll genome evolution glacial bacteria phototrophy rhodopsin |
spellingShingle |
bacteriochlorophyll genome evolution glacial bacteria phototrophy rhodopsin Zeng, Yonghui Chen, Xihan Madsen, Anne Mette Zervas, Athanasios Nielsen, Tue Kjærgaard Andrei, Adrian Stefan Lund-Hansen, Lars Chresten Liu, Yongqin Hansen, Lars Hestbjerg Potential Rhodopsin- and Bacteriochlorophyll-Based Dual Phototrophy in a High Arctic Glacier |
topic_facet |
bacteriochlorophyll genome evolution glacial bacteria phototrophy rhodopsin |
description |
Conserving additional energy from sunlight through bacteriochlorophyll (BChl)-based reaction center or proton-pumping rhodopsin is a highly successful life strategy in environmental bacteria. BChl and rhodopsin-based systems display contrasting characteristics in the size of coding operon, cost of biosynthesis, ease of expression control, and efficiency of energy production. This raises an intriguing question of whether a single bacterium has evolved the ability to perform these two types of phototrophy complementarily according to energy needs and environmental conditions. Here, we report four Tardiphaga sp. strains (Alphaproteobacteria) of monophyletic origin isolated from a high Arctic glacier in northeast Greenland (81.566° N, 16.363° W) that are at different evolutionary stages concerning phototrophy. Their >99.8% identical genomes contain footprints of horizontal operon transfer (HOT) of the complete gene clusters encoding BChl- and xanthorhodopsin (XR)-based dual phototrophy. Two strains possess only a complete XR operon, while the other two strains have both a photosynthesis gene cluster and an XR operon in their genomes. All XR operons are heavily surrounded by mobile genetic elements and are located close to a tRNA gene, strongly signaling that a HOT event of the XR operon has occurred recently. Mining public genome databases and our high Arctic glacial and soil metagenomes revealed that phylogenetically diverse bacteria have the metabolic potential of performing BChl- and rhodopsin-based dual phototrophy. Our data provide new insights on how bacteria cope with the harsh and energy-deficient environment in surface glacier, possibly by maximizing the capability of exploiting solar energy.IMPORTANCE Over the course of evolution for billions of years, bacteria that are capable of light-driven energy production have occupied every corner of surface Earth where sunlight can reach. Only two general biological systems have evolved in bacteria to be capable of net energy conservation via light harvesting: ... |
format |
Article in Journal/Newspaper |
author |
Zeng, Yonghui Chen, Xihan Madsen, Anne Mette Zervas, Athanasios Nielsen, Tue Kjærgaard Andrei, Adrian Stefan Lund-Hansen, Lars Chresten Liu, Yongqin Hansen, Lars Hestbjerg |
author_facet |
Zeng, Yonghui Chen, Xihan Madsen, Anne Mette Zervas, Athanasios Nielsen, Tue Kjærgaard Andrei, Adrian Stefan Lund-Hansen, Lars Chresten Liu, Yongqin Hansen, Lars Hestbjerg |
author_sort |
Zeng, Yonghui |
title |
Potential Rhodopsin- and Bacteriochlorophyll-Based Dual Phototrophy in a High Arctic Glacier |
title_short |
Potential Rhodopsin- and Bacteriochlorophyll-Based Dual Phototrophy in a High Arctic Glacier |
title_full |
Potential Rhodopsin- and Bacteriochlorophyll-Based Dual Phototrophy in a High Arctic Glacier |
title_fullStr |
Potential Rhodopsin- and Bacteriochlorophyll-Based Dual Phototrophy in a High Arctic Glacier |
title_full_unstemmed |
Potential Rhodopsin- and Bacteriochlorophyll-Based Dual Phototrophy in a High Arctic Glacier |
title_sort |
potential rhodopsin- and bacteriochlorophyll-based dual phototrophy in a high arctic glacier |
publishDate |
2020 |
url |
https://pure.au.dk/portal/da/publications/potential-rhodopsin-and-bacteriochlorophyllbased-dual-phototrophy-in-a-high-arctic-glacier(9b4adc0f-7a32-46b1-af0f-33e37ed2d7fa).html https://doi.org/10.1128/mBio.02641-20 http://www.scopus.com/inward/record.url?scp=85096815869&partnerID=8YFLogxK |
geographic |
Arctic Greenland |
geographic_facet |
Arctic Greenland |
genre |
Arctic Arctic glacier Greenland |
genre_facet |
Arctic Arctic glacier Greenland |
op_source |
Zeng , Y , Chen , X , Madsen , A M , Zervas , A , Nielsen , T K , Andrei , A S , Lund-Hansen , L C , Liu , Y & Hansen , L H 2020 , ' Potential Rhodopsin- and Bacteriochlorophyll-Based Dual Phototrophy in a High Arctic Glacier ' , mBio , vol. 11 , no. 6 , e02641-20 , pp. 1-8 . https://doi.org/10.1128/mBio.02641-20 |
op_relation |
https://pure.au.dk/portal/da/publications/potential-rhodopsin-and-bacteriochlorophyllbased-dual-phototrophy-in-a-high-arctic-glacier(9b4adc0f-7a32-46b1-af0f-33e37ed2d7fa).html |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1128/mBio.02641-20 |
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mBio |
container_volume |
11 |
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