Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics
Apoptosis in the testis has two putative roles during normal spermatogenesis; limitation of the germ cell population to numbers that can be supported by the Sertoli cells, and, possibly, selective depletion of meiotic and postmeiotic abnormal germ cells. We investigated the demographic and biologica...
Published in: | Asian Journal of Andrology |
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2012
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Online Access: | https://pure.au.dk/portal/da/publications/relationship-between-apoptotic-markers-in-semen-from-fertile-men-and-demographic-hormonal-and-seminal-characteristics(902dd565-ecc0-49af-8d2d-1cdb49ad4d8a).html https://doi.org/10.1038/aja.2012.76 |
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ftuniaarhuspubl:oai:pure.atira.dk:publications/902dd565-ecc0-49af-8d2d-1cdb49ad4d8a 2023-05-15T16:29:04+02:00 Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics O Specht, Ina Spanò, Marcello S Hougaard, Karin C Manicardi, Gian Bizzaro, Davide Toft, Gunnar Giwercman, Aleksander E Bonde, Jens-Peter 2012-11 https://pure.au.dk/portal/da/publications/relationship-between-apoptotic-markers-in-semen-from-fertile-men-and-demographic-hormonal-and-seminal-characteristics(902dd565-ecc0-49af-8d2d-1cdb49ad4d8a).html https://doi.org/10.1038/aja.2012.76 eng eng info:eu-repo/semantics/restrictedAccess O Specht , I , Spanò , M , S Hougaard , K , C Manicardi , G , Bizzaro , D , Toft , G , Giwercman , A & E Bonde , J-P 2012 , ' Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics ' , Asian Journal of Andrology , vol. 14 , no. 6 , pp. 890-896 . https://doi.org/10.1038/aja.2012.76 article 2012 ftuniaarhuspubl https://doi.org/10.1038/aja.2012.76 2020-11-04T23:43:22Z Apoptosis in the testis has two putative roles during normal spermatogenesis; limitation of the germ cell population to numbers that can be supported by the Sertoli cells, and, possibly, selective depletion of meiotic and postmeiotic abnormal germ cells. We investigated the demographic and biological correlates of the pro-apoptotic marker Fas and the anti-apoptotic marker Bcl-xL in sperm cells of fertile men. Six hundred and four men from Greenland, Poland and Ukraine were consecutively enrolled during their pregnant wife's antenatal visits. Semen analysis was performed as recommended by the World Health Organization. Immunofluorescence coupled to flow cytometry was utilized for detection of apoptotic markers in the sperm cell. DNA damage was assessed by flow cytometry using both the sperm chromatin structure assay (SCSA) and the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay. The percentage of Fas-positive sperm cells was higher in men with high total sperm count (P Article in Journal/Newspaper Greenland Aarhus University: Research Greenland Asian Journal of Andrology 14 6 890 896 |
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Aarhus University: Research |
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ftuniaarhuspubl |
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English |
description |
Apoptosis in the testis has two putative roles during normal spermatogenesis; limitation of the germ cell population to numbers that can be supported by the Sertoli cells, and, possibly, selective depletion of meiotic and postmeiotic abnormal germ cells. We investigated the demographic and biological correlates of the pro-apoptotic marker Fas and the anti-apoptotic marker Bcl-xL in sperm cells of fertile men. Six hundred and four men from Greenland, Poland and Ukraine were consecutively enrolled during their pregnant wife's antenatal visits. Semen analysis was performed as recommended by the World Health Organization. Immunofluorescence coupled to flow cytometry was utilized for detection of apoptotic markers in the sperm cell. DNA damage was assessed by flow cytometry using both the sperm chromatin structure assay (SCSA) and the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay. The percentage of Fas-positive sperm cells was higher in men with high total sperm count (P |
format |
Article in Journal/Newspaper |
author |
O Specht, Ina Spanò, Marcello S Hougaard, Karin C Manicardi, Gian Bizzaro, Davide Toft, Gunnar Giwercman, Aleksander E Bonde, Jens-Peter |
spellingShingle |
O Specht, Ina Spanò, Marcello S Hougaard, Karin C Manicardi, Gian Bizzaro, Davide Toft, Gunnar Giwercman, Aleksander E Bonde, Jens-Peter Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics |
author_facet |
O Specht, Ina Spanò, Marcello S Hougaard, Karin C Manicardi, Gian Bizzaro, Davide Toft, Gunnar Giwercman, Aleksander E Bonde, Jens-Peter |
author_sort |
O Specht, Ina |
title |
Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics |
title_short |
Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics |
title_full |
Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics |
title_fullStr |
Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics |
title_full_unstemmed |
Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics |
title_sort |
relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics |
publishDate |
2012 |
url |
https://pure.au.dk/portal/da/publications/relationship-between-apoptotic-markers-in-semen-from-fertile-men-and-demographic-hormonal-and-seminal-characteristics(902dd565-ecc0-49af-8d2d-1cdb49ad4d8a).html https://doi.org/10.1038/aja.2012.76 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland |
genre_facet |
Greenland |
op_source |
O Specht , I , Spanò , M , S Hougaard , K , C Manicardi , G , Bizzaro , D , Toft , G , Giwercman , A & E Bonde , J-P 2012 , ' Relationship between apoptotic markers in semen from fertile men and demographic, hormonal and seminal characteristics ' , Asian Journal of Andrology , vol. 14 , no. 6 , pp. 890-896 . https://doi.org/10.1038/aja.2012.76 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1038/aja.2012.76 |
container_title |
Asian Journal of Andrology |
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14 |
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6 |
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890 |
op_container_end_page |
896 |
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1766018751576145920 |