Pseudomonas nunensis sp. nov. isolated from a suppressive potato field in Greenland
The bacterial strain In5 T was previously isolated from a suppressive potato field in southern Greenland and has been characterized and described as Pseudomonas fluorescens. However, the results of new polyphasic analyses coupled with those of phenotypic, phylogenetic and genomic analyses reported h...
Published in: | International Journal of Systematic and Evolutionary Microbiology |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2023
|
Subjects: | |
Online Access: | https://pure.au.dk/portal/da/publications/pseudomonas-nunensis-sp-nov-isolated-from-a-suppressive-potato-field-in-greenland(8745c01c-8c9e-45a0-af2a-82c8f67a436b).html https://doi.org/10.1099/ijsem.0.005700 http://www.scopus.com/inward/record.url?scp=85147573158&partnerID=8YFLogxK |
id |
ftuniaarhuspubl:oai:pure.atira.dk:publications/8745c01c-8c9e-45a0-af2a-82c8f67a436b |
---|---|
record_format |
openpolar |
spelling |
ftuniaarhuspubl:oai:pure.atira.dk:publications/8745c01c-8c9e-45a0-af2a-82c8f67a436b 2023-06-11T04:12:15+02:00 Pseudomonas nunensis sp. nov. isolated from a suppressive potato field in Greenland Ntana, Fani Hennessy, Rosanna C. Zervas, Athanasios Stougaard, Peter 2023-02 https://pure.au.dk/portal/da/publications/pseudomonas-nunensis-sp-nov-isolated-from-a-suppressive-potato-field-in-greenland(8745c01c-8c9e-45a0-af2a-82c8f67a436b).html https://doi.org/10.1099/ijsem.0.005700 http://www.scopus.com/inward/record.url?scp=85147573158&partnerID=8YFLogxK eng eng info:eu-repo/semantics/restrictedAccess Ntana , F , Hennessy , R C , Zervas , A & Stougaard , P 2023 , ' Pseudomonas nunensis sp. nov. isolated from a suppressive potato field in Greenland ' , International Journal of Systematic and Evolutionary Microbiology , vol. 73 , no. 2 , 005700 . https://doi.org/10.1099/ijsem.0.005700 antifungal biocontrol phylogenomics potato soil Pseudomonas mandelii subgroup article 2023 ftuniaarhuspubl https://doi.org/10.1099/ijsem.0.005700 2023-05-03T22:57:36Z The bacterial strain In5 T was previously isolated from a suppressive potato field in southern Greenland and has been characterized and described as Pseudomonas fluorescens. However, the results of new polyphasic analyses coupled with those of phenotypic, phylogenetic and genomic analyses reported here demonstrate that the affiliation to the species P. fluorescens was incorrect. The strain is Gram-stain-negative, rod-shaped, aerobic and displays growth at 4–28 °C (optimum temperature 20–25 °C) and at pH 5–9 (optimum pH 6–7). Major fatty acids were C 16: 0 (38.2 %), a summed feature consisting of C 16: 1 ω6c and/or C 16: 1 ω7c) (20.7 %), C 17: 0 cyclo ω7c (14.3 %) and a summed feature consisting of C 18: 1 ω6c and/or C 18: 1 ω7c (11.7 %). The respiratory quinones were determined to be Q9 (95.5 %) and Q8 (4.5 %) and major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglyc-erol. The DNA G+C content was determined to be 59.4 mol%. The results of phylogenetic analysis based on the 16S rRNA gene and multi-locus sequence analysis (MLSA; concatenated 16S rRNA, gyrB, rpoB and rpoD sequences) indicated that In5 T was affiliated with the Pseudomonas mandelii subgroup within the genus Pseudomonas. Comparison of the genome sequence of In5 T and those of related type strains of species of the genus Pseudomonas revealed an average nucleotide identity (ANI) of 87.7 % or less and digital DNA–DNA hybridization (dDDH) of less than 34.5 % relatedness, respectively. Two more strains, In614 and In655, isolated from the same suppressive soil were included in the genome analysis. The ANI and dDDH of In614 and In655 compared with In5 T were ANI: 99.9 and 97.6 and dDDH (GGDC) 99.9 and 79.4, respectively, indicating that In5 T , In614 and In655 are representatives of the same species. The results of the phenotypic, phylogenetic and genomic analyses support the hypothesis that strain In5 T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas nunensis sp. nov. is proposed. ... Article in Journal/Newspaper Greenland Aarhus University: Research Greenland International Journal of Systematic and Evolutionary Microbiology 73 2 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
antifungal biocontrol phylogenomics potato soil Pseudomonas mandelii subgroup |
spellingShingle |
antifungal biocontrol phylogenomics potato soil Pseudomonas mandelii subgroup Ntana, Fani Hennessy, Rosanna C. Zervas, Athanasios Stougaard, Peter Pseudomonas nunensis sp. nov. isolated from a suppressive potato field in Greenland |
topic_facet |
antifungal biocontrol phylogenomics potato soil Pseudomonas mandelii subgroup |
description |
The bacterial strain In5 T was previously isolated from a suppressive potato field in southern Greenland and has been characterized and described as Pseudomonas fluorescens. However, the results of new polyphasic analyses coupled with those of phenotypic, phylogenetic and genomic analyses reported here demonstrate that the affiliation to the species P. fluorescens was incorrect. The strain is Gram-stain-negative, rod-shaped, aerobic and displays growth at 4–28 °C (optimum temperature 20–25 °C) and at pH 5–9 (optimum pH 6–7). Major fatty acids were C 16: 0 (38.2 %), a summed feature consisting of C 16: 1 ω6c and/or C 16: 1 ω7c) (20.7 %), C 17: 0 cyclo ω7c (14.3 %) and a summed feature consisting of C 18: 1 ω6c and/or C 18: 1 ω7c (11.7 %). The respiratory quinones were determined to be Q9 (95.5 %) and Q8 (4.5 %) and major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglyc-erol. The DNA G+C content was determined to be 59.4 mol%. The results of phylogenetic analysis based on the 16S rRNA gene and multi-locus sequence analysis (MLSA; concatenated 16S rRNA, gyrB, rpoB and rpoD sequences) indicated that In5 T was affiliated with the Pseudomonas mandelii subgroup within the genus Pseudomonas. Comparison of the genome sequence of In5 T and those of related type strains of species of the genus Pseudomonas revealed an average nucleotide identity (ANI) of 87.7 % or less and digital DNA–DNA hybridization (dDDH) of less than 34.5 % relatedness, respectively. Two more strains, In614 and In655, isolated from the same suppressive soil were included in the genome analysis. The ANI and dDDH of In614 and In655 compared with In5 T were ANI: 99.9 and 97.6 and dDDH (GGDC) 99.9 and 79.4, respectively, indicating that In5 T , In614 and In655 are representatives of the same species. The results of the phenotypic, phylogenetic and genomic analyses support the hypothesis that strain In5 T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas nunensis sp. nov. is proposed. ... |
format |
Article in Journal/Newspaper |
author |
Ntana, Fani Hennessy, Rosanna C. Zervas, Athanasios Stougaard, Peter |
author_facet |
Ntana, Fani Hennessy, Rosanna C. Zervas, Athanasios Stougaard, Peter |
author_sort |
Ntana, Fani |
title |
Pseudomonas nunensis sp. nov. isolated from a suppressive potato field in Greenland |
title_short |
Pseudomonas nunensis sp. nov. isolated from a suppressive potato field in Greenland |
title_full |
Pseudomonas nunensis sp. nov. isolated from a suppressive potato field in Greenland |
title_fullStr |
Pseudomonas nunensis sp. nov. isolated from a suppressive potato field in Greenland |
title_full_unstemmed |
Pseudomonas nunensis sp. nov. isolated from a suppressive potato field in Greenland |
title_sort |
pseudomonas nunensis sp. nov. isolated from a suppressive potato field in greenland |
publishDate |
2023 |
url |
https://pure.au.dk/portal/da/publications/pseudomonas-nunensis-sp-nov-isolated-from-a-suppressive-potato-field-in-greenland(8745c01c-8c9e-45a0-af2a-82c8f67a436b).html https://doi.org/10.1099/ijsem.0.005700 http://www.scopus.com/inward/record.url?scp=85147573158&partnerID=8YFLogxK |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland |
genre_facet |
Greenland |
op_source |
Ntana , F , Hennessy , R C , Zervas , A & Stougaard , P 2023 , ' Pseudomonas nunensis sp. nov. isolated from a suppressive potato field in Greenland ' , International Journal of Systematic and Evolutionary Microbiology , vol. 73 , no. 2 , 005700 . https://doi.org/10.1099/ijsem.0.005700 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1099/ijsem.0.005700 |
container_title |
International Journal of Systematic and Evolutionary Microbiology |
container_volume |
73 |
container_issue |
2 |
_version_ |
1768387984537681920 |