The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought
The springtail Megaphorura arctica Tullberg 1876 is widespread in the arctic and subarctic regions where it can be abundant along beaches. This species survives winters using cryoprotective dehydration as a cold tolerance strategy during which it becomes drastically dehydrated. Several studies have...
Published in: | Journal of Comparative Physiology B |
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ftuniaarhuspubl:oai:pure.atira.dk:publications/858aef79-10df-4650-9735-1f22291b0904 2023-05-15T15:12:42+02:00 The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought Holmstrup, Martin 2018-11 https://pure.au.dk/portal/da/publications/the-springtail-megaphorura-arctica-survives-extremely-high-osmolality-of-body-fluids-during-drought(858aef79-10df-4650-9735-1f22291b0904).html https://doi.org/10.1007/s00360-018-1180-3 eng eng info:eu-repo/semantics/closedAccess Holmstrup , M 2018 , ' The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought ' , Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology , vol. 188 , no. 6 , pp. 939-945 . https://doi.org/10.1007/s00360-018-1180-3 Anhydrobiosis Collembola Desiccation Differential scanning calorimetry Osmotic response TULLBERG 1876 ONYCHIURIDAE WATER-VAPOR ABSORPTION CRYOPROTECTIVE DEHYDRATION FOLSOMIA-CANDIDA TOLERANCE COLD ACCUMULATION article 2018 ftuniaarhuspubl https://doi.org/10.1007/s00360-018-1180-3 2020-07-18T22:09:29Z The springtail Megaphorura arctica Tullberg 1876 is widespread in the arctic and subarctic regions where it can be abundant along beaches. This species survives winters using cryoprotective dehydration as a cold tolerance strategy during which it becomes drastically dehydrated. Several studies have investigated the physiological responses associated with water loss of M. arctica under exposure to freezing temperatures, but little is known of the dynamics of body water and hemolymph osmolality when subjected to gradually increasing drought stress at temperatures above the freezing point. Therefore, an experiment was conducted in which M. arctica was subjected to relative humidities (RH) decreasing from fully saturated conditions to ca. 89%RH over a period of 30 days. During the experiment water content of springtails decreased from about 3 to ca. 0.5mgmg(-1) dry weight. Alongside with water loss, trehalose concentrations increased from nearly nothing to 0.12mg mg(-1) dry weight, which contributed to an increase in hemolymph osmolality from ca. 250mOsm to at least 7Osm. All springtails survived water loss down to 0.7mgmg(-1) dry weight and hemolymph osmolality of ca. 4Osm, and about 60% of the springtails survived with only 0.5mg watermg(-1) dry weight and osmolality of ca. 7Osm.At this level of dehydration, Differential Scanning Calorimetry analysis showed that most, but not all, osmotically active water was lost. It is discussed that the extensive dehydration must be associated with high concentrations of salts potentially causing denaturation and precipitation of cellular proteins. M. arctica is remarkably tolerant of dehydration, but because it does not endure loss of the osmotically inactive water it cannot be categorized as a truly anhydrobiotic species. Article in Journal/Newspaper Arctic Subarctic Springtail Aarhus University: Research Arctic Journal of Comparative Physiology B 188 6 939 945 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
Anhydrobiosis Collembola Desiccation Differential scanning calorimetry Osmotic response TULLBERG 1876 ONYCHIURIDAE WATER-VAPOR ABSORPTION CRYOPROTECTIVE DEHYDRATION FOLSOMIA-CANDIDA TOLERANCE COLD ACCUMULATION |
spellingShingle |
Anhydrobiosis Collembola Desiccation Differential scanning calorimetry Osmotic response TULLBERG 1876 ONYCHIURIDAE WATER-VAPOR ABSORPTION CRYOPROTECTIVE DEHYDRATION FOLSOMIA-CANDIDA TOLERANCE COLD ACCUMULATION Holmstrup, Martin The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought |
topic_facet |
Anhydrobiosis Collembola Desiccation Differential scanning calorimetry Osmotic response TULLBERG 1876 ONYCHIURIDAE WATER-VAPOR ABSORPTION CRYOPROTECTIVE DEHYDRATION FOLSOMIA-CANDIDA TOLERANCE COLD ACCUMULATION |
description |
The springtail Megaphorura arctica Tullberg 1876 is widespread in the arctic and subarctic regions where it can be abundant along beaches. This species survives winters using cryoprotective dehydration as a cold tolerance strategy during which it becomes drastically dehydrated. Several studies have investigated the physiological responses associated with water loss of M. arctica under exposure to freezing temperatures, but little is known of the dynamics of body water and hemolymph osmolality when subjected to gradually increasing drought stress at temperatures above the freezing point. Therefore, an experiment was conducted in which M. arctica was subjected to relative humidities (RH) decreasing from fully saturated conditions to ca. 89%RH over a period of 30 days. During the experiment water content of springtails decreased from about 3 to ca. 0.5mgmg(-1) dry weight. Alongside with water loss, trehalose concentrations increased from nearly nothing to 0.12mg mg(-1) dry weight, which contributed to an increase in hemolymph osmolality from ca. 250mOsm to at least 7Osm. All springtails survived water loss down to 0.7mgmg(-1) dry weight and hemolymph osmolality of ca. 4Osm, and about 60% of the springtails survived with only 0.5mg watermg(-1) dry weight and osmolality of ca. 7Osm.At this level of dehydration, Differential Scanning Calorimetry analysis showed that most, but not all, osmotically active water was lost. It is discussed that the extensive dehydration must be associated with high concentrations of salts potentially causing denaturation and precipitation of cellular proteins. M. arctica is remarkably tolerant of dehydration, but because it does not endure loss of the osmotically inactive water it cannot be categorized as a truly anhydrobiotic species. |
format |
Article in Journal/Newspaper |
author |
Holmstrup, Martin |
author_facet |
Holmstrup, Martin |
author_sort |
Holmstrup, Martin |
title |
The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought |
title_short |
The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought |
title_full |
The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought |
title_fullStr |
The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought |
title_full_unstemmed |
The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought |
title_sort |
springtail megaphorura arctica survives extremely high osmolality of body fluids during drought |
publishDate |
2018 |
url |
https://pure.au.dk/portal/da/publications/the-springtail-megaphorura-arctica-survives-extremely-high-osmolality-of-body-fluids-during-drought(858aef79-10df-4650-9735-1f22291b0904).html https://doi.org/10.1007/s00360-018-1180-3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Subarctic Springtail |
genre_facet |
Arctic Subarctic Springtail |
op_source |
Holmstrup , M 2018 , ' The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought ' , Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology , vol. 188 , no. 6 , pp. 939-945 . https://doi.org/10.1007/s00360-018-1180-3 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1007/s00360-018-1180-3 |
container_title |
Journal of Comparative Physiology B |
container_volume |
188 |
container_issue |
6 |
container_start_page |
939 |
op_container_end_page |
945 |
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1766343354271924224 |