Microbial community structure of Arctic multiyear sea ice and surface seawater by 454 sequencing of the 16S RNA gene
Dramatic decreases in the extent of Arctic multiyear ice (MYI) suggest this environment may disappear as early as 2100, replaced by ecologically different first-year ice. To better understand the implications of this loss on microbial biodiversity, we undertook a detailed census of the microbial com...
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2012
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Online Access: | https://pure.au.dk/portal/da/publications/microbial-community-structure-of-arctic-multiyear-sea-ice-and-surface-seawater-by-454-sequencing-of-the-16s-rna-gene(820160db-bbbc-4a4e-8c25-7364c1ddce17).html https://doi.org/10.1038/ismej.2011.76 http://www.scopus.com/inward/record.url?eid=2-s2.0-84355162255&partnerID=40&md5=984bb37c592eeeacab33d427c0975b91 |
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ftuniaarhuspubl:oai:pure.atira.dk:publications/820160db-bbbc-4a4e-8c25-7364c1ddce17 2023-05-15T14:25:54+02:00 Microbial community structure of Arctic multiyear sea ice and surface seawater by 454 sequencing of the 16S RNA gene Bowman, J.S. Rasmussen, S. Blom, Nikolaj Deming, J.W. Rysgaard, Søren Sicheritz-Ponten, T. 2012 https://pure.au.dk/portal/da/publications/microbial-community-structure-of-arctic-multiyear-sea-ice-and-surface-seawater-by-454-sequencing-of-the-16s-rna-gene(820160db-bbbc-4a4e-8c25-7364c1ddce17).html https://doi.org/10.1038/ismej.2011.76 http://www.scopus.com/inward/record.url?eid=2-s2.0-84355162255&partnerID=40&md5=984bb37c592eeeacab33d427c0975b91 eng eng info:eu-repo/semantics/restrictedAccess Bowman , J S , Rasmussen , S , Blom , N , Deming , J W , Rysgaard , S & Sicheritz-Ponten , T 2012 , ' Microbial community structure of Arctic multiyear sea ice and surface seawater by 454 sequencing of the 16S RNA gene ' , I S M E Journal , vol. 6 , no. 1 , pp. 11-20 . https://doi.org/10.1038/ismej.2011.76 454 pyrosequencing multiyear sea ice sea ice bacteria RNA 16S sea water biodiversity cladistics cyanobacterium gene expression genetic analysis microbial community niche sea ice seawater species richness archaeon Arctic article bacterium genetics high throughput sequencing ice cover isolation and purification methodology microbiology phylogeny Archaea Arctic Regions Bacteria High-Throughput Nucleotide Sequencing RNA Ribosomal 16S Bacteria (microorganisms) Cyanobacteria Spartobacteria article 2012 ftuniaarhuspubl https://doi.org/10.1038/ismej.2011.76 2020-07-18T21:23:45Z Dramatic decreases in the extent of Arctic multiyear ice (MYI) suggest this environment may disappear as early as 2100, replaced by ecologically different first-year ice. To better understand the implications of this loss on microbial biodiversity, we undertook a detailed census of the microbial community in MYI at two sites near the geographic North Pole using parallel tag sequencing of the 16S rRNA gene. Although the composition of the MYI microbial community has been characterized by previous studies, microbial community structure has not been. Although richness was lower in MYI than in underlying surface water, we found diversity to be comparable using the Simpson and Shannon's indices (for Simpson t=0.65, P=0.56; for Shannon t=0.25, P=0.84 for a Student's t-test of mean values). Cyanobacteria, comprising 6.8% of reads obtained from MYI, were observed for the first time in Arctic sea ice. In addition, several low-abundance clades not previously reported in sea ice were present, including the phylum TM7 and the classes Spartobacteria and Opitutae. Members of Coraliomargarita, a recently described genus of the class Opitutae, were present in sufficient numbers to suggest niche occupation within MYI. © 2012 International Society for Microbial Ecology All rights reserved. Article in Journal/Newspaper Arctic Arctic North Pole Sea ice Aarhus University: Research Arctic North Pole The ISME Journal 6 1 11 20 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
454 pyrosequencing multiyear sea ice sea ice bacteria RNA 16S sea water biodiversity cladistics cyanobacterium gene expression genetic analysis microbial community niche sea ice seawater species richness archaeon Arctic article bacterium genetics high throughput sequencing ice cover isolation and purification methodology microbiology phylogeny Archaea Arctic Regions Bacteria High-Throughput Nucleotide Sequencing RNA Ribosomal 16S Bacteria (microorganisms) Cyanobacteria Spartobacteria |
spellingShingle |
454 pyrosequencing multiyear sea ice sea ice bacteria RNA 16S sea water biodiversity cladistics cyanobacterium gene expression genetic analysis microbial community niche sea ice seawater species richness archaeon Arctic article bacterium genetics high throughput sequencing ice cover isolation and purification methodology microbiology phylogeny Archaea Arctic Regions Bacteria High-Throughput Nucleotide Sequencing RNA Ribosomal 16S Bacteria (microorganisms) Cyanobacteria Spartobacteria Bowman, J.S. Rasmussen, S. Blom, Nikolaj Deming, J.W. Rysgaard, Søren Sicheritz-Ponten, T. Microbial community structure of Arctic multiyear sea ice and surface seawater by 454 sequencing of the 16S RNA gene |
topic_facet |
454 pyrosequencing multiyear sea ice sea ice bacteria RNA 16S sea water biodiversity cladistics cyanobacterium gene expression genetic analysis microbial community niche sea ice seawater species richness archaeon Arctic article bacterium genetics high throughput sequencing ice cover isolation and purification methodology microbiology phylogeny Archaea Arctic Regions Bacteria High-Throughput Nucleotide Sequencing RNA Ribosomal 16S Bacteria (microorganisms) Cyanobacteria Spartobacteria |
description |
Dramatic decreases in the extent of Arctic multiyear ice (MYI) suggest this environment may disappear as early as 2100, replaced by ecologically different first-year ice. To better understand the implications of this loss on microbial biodiversity, we undertook a detailed census of the microbial community in MYI at two sites near the geographic North Pole using parallel tag sequencing of the 16S rRNA gene. Although the composition of the MYI microbial community has been characterized by previous studies, microbial community structure has not been. Although richness was lower in MYI than in underlying surface water, we found diversity to be comparable using the Simpson and Shannon's indices (for Simpson t=0.65, P=0.56; for Shannon t=0.25, P=0.84 for a Student's t-test of mean values). Cyanobacteria, comprising 6.8% of reads obtained from MYI, were observed for the first time in Arctic sea ice. In addition, several low-abundance clades not previously reported in sea ice were present, including the phylum TM7 and the classes Spartobacteria and Opitutae. Members of Coraliomargarita, a recently described genus of the class Opitutae, were present in sufficient numbers to suggest niche occupation within MYI. © 2012 International Society for Microbial Ecology All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Bowman, J.S. Rasmussen, S. Blom, Nikolaj Deming, J.W. Rysgaard, Søren Sicheritz-Ponten, T. |
author_facet |
Bowman, J.S. Rasmussen, S. Blom, Nikolaj Deming, J.W. Rysgaard, Søren Sicheritz-Ponten, T. |
author_sort |
Bowman, J.S. |
title |
Microbial community structure of Arctic multiyear sea ice and surface seawater by 454 sequencing of the 16S RNA gene |
title_short |
Microbial community structure of Arctic multiyear sea ice and surface seawater by 454 sequencing of the 16S RNA gene |
title_full |
Microbial community structure of Arctic multiyear sea ice and surface seawater by 454 sequencing of the 16S RNA gene |
title_fullStr |
Microbial community structure of Arctic multiyear sea ice and surface seawater by 454 sequencing of the 16S RNA gene |
title_full_unstemmed |
Microbial community structure of Arctic multiyear sea ice and surface seawater by 454 sequencing of the 16S RNA gene |
title_sort |
microbial community structure of arctic multiyear sea ice and surface seawater by 454 sequencing of the 16s rna gene |
publishDate |
2012 |
url |
https://pure.au.dk/portal/da/publications/microbial-community-structure-of-arctic-multiyear-sea-ice-and-surface-seawater-by-454-sequencing-of-the-16s-rna-gene(820160db-bbbc-4a4e-8c25-7364c1ddce17).html https://doi.org/10.1038/ismej.2011.76 http://www.scopus.com/inward/record.url?eid=2-s2.0-84355162255&partnerID=40&md5=984bb37c592eeeacab33d427c0975b91 |
geographic |
Arctic North Pole |
geographic_facet |
Arctic North Pole |
genre |
Arctic Arctic North Pole Sea ice |
genre_facet |
Arctic Arctic North Pole Sea ice |
op_source |
Bowman , J S , Rasmussen , S , Blom , N , Deming , J W , Rysgaard , S & Sicheritz-Ponten , T 2012 , ' Microbial community structure of Arctic multiyear sea ice and surface seawater by 454 sequencing of the 16S RNA gene ' , I S M E Journal , vol. 6 , no. 1 , pp. 11-20 . https://doi.org/10.1038/ismej.2011.76 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1038/ismej.2011.76 |
container_title |
The ISME Journal |
container_volume |
6 |
container_issue |
1 |
container_start_page |
11 |
op_container_end_page |
20 |
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1766298376153858048 |