A comparison of low temperature biology of Pieris rapae from Ontario, Canada, and Yakutia, Far Eastern Russia
Low temperatures limit the distribution and abundance of ectotherms. However, many insects can survive low temperatures by employing one of two cold tolerance strategies: freeze avoidance or freeze tolerance. Very few species can employ both strategies, but those that do provide a rare opportunity t...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2020
|
Subjects: | |
Online Access: | https://pure.au.dk/portal/da/publications/a-comparison-of-low-temperature-biology-of-pieris-rapae-from-ontario-canada-and-yakutia-far-eastern-russia(7b09b167-fbe6-4e2f-8b8f-df7cdc9bf16b).html https://doi.org/10.1016/j.cbpa.2020.110649 http://www.scopus.com/inward/record.url?scp=85077919543&partnerID=8YFLogxK |
id |
ftuniaarhuspubl:oai:pure.atira.dk:publications/7b09b167-fbe6-4e2f-8b8f-df7cdc9bf16b |
---|---|
record_format |
openpolar |
spelling |
ftuniaarhuspubl:oai:pure.atira.dk:publications/7b09b167-fbe6-4e2f-8b8f-df7cdc9bf16b 2023-05-15T18:45:07+02:00 A comparison of low temperature biology of Pieris rapae from Ontario, Canada, and Yakutia, Far Eastern Russia Li, Natalia G. Toxopeus, Jantina Moos, Martin Sørensen, Jesper G. Sinclair, Brent J. 2020-04 https://pure.au.dk/portal/da/publications/a-comparison-of-low-temperature-biology-of-pieris-rapae-from-ontario-canada-and-yakutia-far-eastern-russia(7b09b167-fbe6-4e2f-8b8f-df7cdc9bf16b).html https://doi.org/10.1016/j.cbpa.2020.110649 http://www.scopus.com/inward/record.url?scp=85077919543&partnerID=8YFLogxK eng eng info:eu-repo/semantics/restrictedAccess Li , N G , Toxopeus , J , Moos , M , Sørensen , J G & Sinclair , B J 2020 , ' A comparison of low temperature biology of Pieris rapae from Ontario, Canada, and Yakutia, Far Eastern Russia ' , Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology , vol. 242 , 110649 . https://doi.org/10.1016/j.cbpa.2020.110649 Cryoprotectants Freeze avoidance Freeze tolerance Metabolomics Plasticity article 2020 ftuniaarhuspubl https://doi.org/10.1016/j.cbpa.2020.110649 2020-07-18T22:20:46Z Low temperatures limit the distribution and abundance of ectotherms. However, many insects can survive low temperatures by employing one of two cold tolerance strategies: freeze avoidance or freeze tolerance. Very few species can employ both strategies, but those that do provide a rare opportunity to study the mechanisms that differentiate freeze tolerance and freeze avoidance. We showed that overwintering pupae of the cabbage white butterfly Pieris rapae can be freeze tolerant or freeze avoidant. Pupae from a population of P. rapae in northeastern Russia (Yakutsk) froze at c. −9.3 °C and were freeze-tolerant in 2002–2003 when overwintered outside. However, P. rapae from both Yakutsk and southern Canada (London) acclimated to milder laboratory conditions in 2014 and 2017 froze at lower temperatures (< −20 °C) and were freeze-avoidant. Summer-collected P. rapae larvae (collected in Yakutsk in 2016) were partially freeze-tolerant, and decreased the temperature at which they froze in response to starvation at mild low temperatures (4 °C) and repeated partial freezing events. By comparing similarly-acclimated P. rapae pupae from both populations, we identified molecules that may facilitate low temperature tolerance, including the hemolymph ice-binding molecules and several potential low molecular weight cryoprotectants. Pieris rapae from Yakutsk exhibited high physiological plasticity, accumulating cryoprotectants and almost doubling their hemolymph osmolality when supercooled to −15 °C for two weeks, while the London P. rapae population exhibited minimal plasticity. We hypothesize that physiological plasticity is an important adaptation to extreme low temperatures (i.e. in Yakutsk) and may facilitate the transition between freeze avoidance and freeze tolerance. Article in Journal/Newspaper Yakutia Yakutsk Aarhus University: Research Canada Yakutsk Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 242 110649 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
Cryoprotectants Freeze avoidance Freeze tolerance Metabolomics Plasticity |
spellingShingle |
Cryoprotectants Freeze avoidance Freeze tolerance Metabolomics Plasticity Li, Natalia G. Toxopeus, Jantina Moos, Martin Sørensen, Jesper G. Sinclair, Brent J. A comparison of low temperature biology of Pieris rapae from Ontario, Canada, and Yakutia, Far Eastern Russia |
topic_facet |
Cryoprotectants Freeze avoidance Freeze tolerance Metabolomics Plasticity |
description |
Low temperatures limit the distribution and abundance of ectotherms. However, many insects can survive low temperatures by employing one of two cold tolerance strategies: freeze avoidance or freeze tolerance. Very few species can employ both strategies, but those that do provide a rare opportunity to study the mechanisms that differentiate freeze tolerance and freeze avoidance. We showed that overwintering pupae of the cabbage white butterfly Pieris rapae can be freeze tolerant or freeze avoidant. Pupae from a population of P. rapae in northeastern Russia (Yakutsk) froze at c. −9.3 °C and were freeze-tolerant in 2002–2003 when overwintered outside. However, P. rapae from both Yakutsk and southern Canada (London) acclimated to milder laboratory conditions in 2014 and 2017 froze at lower temperatures (< −20 °C) and were freeze-avoidant. Summer-collected P. rapae larvae (collected in Yakutsk in 2016) were partially freeze-tolerant, and decreased the temperature at which they froze in response to starvation at mild low temperatures (4 °C) and repeated partial freezing events. By comparing similarly-acclimated P. rapae pupae from both populations, we identified molecules that may facilitate low temperature tolerance, including the hemolymph ice-binding molecules and several potential low molecular weight cryoprotectants. Pieris rapae from Yakutsk exhibited high physiological plasticity, accumulating cryoprotectants and almost doubling their hemolymph osmolality when supercooled to −15 °C for two weeks, while the London P. rapae population exhibited minimal plasticity. We hypothesize that physiological plasticity is an important adaptation to extreme low temperatures (i.e. in Yakutsk) and may facilitate the transition between freeze avoidance and freeze tolerance. |
format |
Article in Journal/Newspaper |
author |
Li, Natalia G. Toxopeus, Jantina Moos, Martin Sørensen, Jesper G. Sinclair, Brent J. |
author_facet |
Li, Natalia G. Toxopeus, Jantina Moos, Martin Sørensen, Jesper G. Sinclair, Brent J. |
author_sort |
Li, Natalia G. |
title |
A comparison of low temperature biology of Pieris rapae from Ontario, Canada, and Yakutia, Far Eastern Russia |
title_short |
A comparison of low temperature biology of Pieris rapae from Ontario, Canada, and Yakutia, Far Eastern Russia |
title_full |
A comparison of low temperature biology of Pieris rapae from Ontario, Canada, and Yakutia, Far Eastern Russia |
title_fullStr |
A comparison of low temperature biology of Pieris rapae from Ontario, Canada, and Yakutia, Far Eastern Russia |
title_full_unstemmed |
A comparison of low temperature biology of Pieris rapae from Ontario, Canada, and Yakutia, Far Eastern Russia |
title_sort |
comparison of low temperature biology of pieris rapae from ontario, canada, and yakutia, far eastern russia |
publishDate |
2020 |
url |
https://pure.au.dk/portal/da/publications/a-comparison-of-low-temperature-biology-of-pieris-rapae-from-ontario-canada-and-yakutia-far-eastern-russia(7b09b167-fbe6-4e2f-8b8f-df7cdc9bf16b).html https://doi.org/10.1016/j.cbpa.2020.110649 http://www.scopus.com/inward/record.url?scp=85077919543&partnerID=8YFLogxK |
geographic |
Canada Yakutsk |
geographic_facet |
Canada Yakutsk |
genre |
Yakutia Yakutsk |
genre_facet |
Yakutia Yakutsk |
op_source |
Li , N G , Toxopeus , J , Moos , M , Sørensen , J G & Sinclair , B J 2020 , ' A comparison of low temperature biology of Pieris rapae from Ontario, Canada, and Yakutia, Far Eastern Russia ' , Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology , vol. 242 , 110649 . https://doi.org/10.1016/j.cbpa.2020.110649 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.cbpa.2020.110649 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
242 |
container_start_page |
110649 |
_version_ |
1766236092147695616 |