Fructan accumulation and transcription of candidate genes during cold acclimation in three varieties of Poa pratensis
Poa pratensis , a type species for the grass family ( Poaceae ), is an important cool season grass that accumulates fructans as a polysaccharide reserve. We studied fructan contents and expression of candidate fructan metabolism genes during cold acclimation in three varieties of P. pratensis adapte...
Published in: | Journal of Plant Physiology |
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2011
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ftuniaarhuspubl:oai:pure.atira.dk:publications/6d13bfe0-3726-11df-8b17-000ea68e967b 2023-05-15T17:43:39+02:00 Fructan accumulation and transcription of candidate genes during cold acclimation in three varieties of Poa pratensis Rao, R Shyama Prasad Andersen, Jeppe Reitan Dionisio, Giuseppe Boelt, Birte 2011-03 https://pure.au.dk/portal/da/publications/fructan-accumulation-and-transcription-of-candidate-genes-during-cold-acclimation-in-three-varieties-of-poa-pratensis(6d13bfe0-3726-11df-8b17-000ea68e967b).html https://doi.org/10.1016/j.jplph.2010.07.019 eng eng info:eu-repo/semantics/restrictedAccess Rao , R S P , Andersen , J R , Dionisio , G & Boelt , B 2011 , ' Fructan accumulation and transcription of candidate genes during cold acclimation in three varieties of Poa pratensis ' , Journal of Plant Physiology , vol. 168 , no. 4 , pp. 344-351 . https://doi.org/10.1016/j.jplph.2010.07.019 cold acclimation fructan exohydrolase fructosyltransferase gene expression poa pratensis article 2011 ftuniaarhuspubl https://doi.org/10.1016/j.jplph.2010.07.019 2023-03-08T23:55:22Z Poa pratensis , a type species for the grass family ( Poaceae ), is an important cool season grass that accumulates fructans as a polysaccharide reserve. We studied fructan contents and expression of candidate fructan metabolism genes during cold acclimation in three varieties of P. pratensis adapted to different environments: Northern Norway, Denmark, and the Netherlands. Fructan content increased significantly during cold acclimation and varieties showed significant differences in the level of fructan accumulation. cDNA sequences of putative fructosyltransferase ( FT ), fructan exohydrolase ( FEH ), and cold acclimation protein ( CAP ) genes were identified and cloned. In agreement with a function in fructan biosynthesis, transcription of a putative sucrose:fructan 6-fructosyltransferase ( Pp6-SFT ) gene was induced during cold acclimation and fructan accumulation in all three P. pratensis varieties. Transcription of putative PpFEH and PpCAP genes was also induced by cold acclimation; however, transcription of these two genes was several-fold higher in the variety from Norway compared to the other two varieties. The results presented here suggest that Pp6-SFT is involved in fructan biosynthesis in P. pratensis . FEHs have previously been suggested to be involved in fructan biosynthesis and freezing tolerance, and induced expression of PpFEH during fructan accumulation could also suggest a role in fructan biosynthesis. However, based on the different PpFEH transcription rates among varieties and similar expression of PpFEH and PpCAP , we suggest that PpFEH is more likely to be involved in mediating freezing tolerance, e.g., by regulating the cell osmotic potential through fructan degradation. Poa pratensis , a type species for the grass family ( Poaceae ), is an important cool season grass that accumulates fructans as a polysaccharide reserve. We studied fructan contents and expression of candidate fructan metabolism genes during cold acclimation in three varieties of P. pratensis adapted to different ... Article in Journal/Newspaper Northern Norway Aarhus University: Research Norway Journal of Plant Physiology 168 4 344 351 |
institution |
Open Polar |
collection |
Aarhus University: Research |
op_collection_id |
ftuniaarhuspubl |
language |
English |
topic |
cold acclimation fructan exohydrolase fructosyltransferase gene expression poa pratensis |
spellingShingle |
cold acclimation fructan exohydrolase fructosyltransferase gene expression poa pratensis Rao, R Shyama Prasad Andersen, Jeppe Reitan Dionisio, Giuseppe Boelt, Birte Fructan accumulation and transcription of candidate genes during cold acclimation in three varieties of Poa pratensis |
topic_facet |
cold acclimation fructan exohydrolase fructosyltransferase gene expression poa pratensis |
description |
Poa pratensis , a type species for the grass family ( Poaceae ), is an important cool season grass that accumulates fructans as a polysaccharide reserve. We studied fructan contents and expression of candidate fructan metabolism genes during cold acclimation in three varieties of P. pratensis adapted to different environments: Northern Norway, Denmark, and the Netherlands. Fructan content increased significantly during cold acclimation and varieties showed significant differences in the level of fructan accumulation. cDNA sequences of putative fructosyltransferase ( FT ), fructan exohydrolase ( FEH ), and cold acclimation protein ( CAP ) genes were identified and cloned. In agreement with a function in fructan biosynthesis, transcription of a putative sucrose:fructan 6-fructosyltransferase ( Pp6-SFT ) gene was induced during cold acclimation and fructan accumulation in all three P. pratensis varieties. Transcription of putative PpFEH and PpCAP genes was also induced by cold acclimation; however, transcription of these two genes was several-fold higher in the variety from Norway compared to the other two varieties. The results presented here suggest that Pp6-SFT is involved in fructan biosynthesis in P. pratensis . FEHs have previously been suggested to be involved in fructan biosynthesis and freezing tolerance, and induced expression of PpFEH during fructan accumulation could also suggest a role in fructan biosynthesis. However, based on the different PpFEH transcription rates among varieties and similar expression of PpFEH and PpCAP , we suggest that PpFEH is more likely to be involved in mediating freezing tolerance, e.g., by regulating the cell osmotic potential through fructan degradation. Poa pratensis , a type species for the grass family ( Poaceae ), is an important cool season grass that accumulates fructans as a polysaccharide reserve. We studied fructan contents and expression of candidate fructan metabolism genes during cold acclimation in three varieties of P. pratensis adapted to different ... |
format |
Article in Journal/Newspaper |
author |
Rao, R Shyama Prasad Andersen, Jeppe Reitan Dionisio, Giuseppe Boelt, Birte |
author_facet |
Rao, R Shyama Prasad Andersen, Jeppe Reitan Dionisio, Giuseppe Boelt, Birte |
author_sort |
Rao, R Shyama Prasad |
title |
Fructan accumulation and transcription of candidate genes during cold acclimation in three varieties of Poa pratensis |
title_short |
Fructan accumulation and transcription of candidate genes during cold acclimation in three varieties of Poa pratensis |
title_full |
Fructan accumulation and transcription of candidate genes during cold acclimation in three varieties of Poa pratensis |
title_fullStr |
Fructan accumulation and transcription of candidate genes during cold acclimation in three varieties of Poa pratensis |
title_full_unstemmed |
Fructan accumulation and transcription of candidate genes during cold acclimation in three varieties of Poa pratensis |
title_sort |
fructan accumulation and transcription of candidate genes during cold acclimation in three varieties of poa pratensis |
publishDate |
2011 |
url |
https://pure.au.dk/portal/da/publications/fructan-accumulation-and-transcription-of-candidate-genes-during-cold-acclimation-in-three-varieties-of-poa-pratensis(6d13bfe0-3726-11df-8b17-000ea68e967b).html https://doi.org/10.1016/j.jplph.2010.07.019 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Northern Norway |
genre_facet |
Northern Norway |
op_source |
Rao , R S P , Andersen , J R , Dionisio , G & Boelt , B 2011 , ' Fructan accumulation and transcription of candidate genes during cold acclimation in three varieties of Poa pratensis ' , Journal of Plant Physiology , vol. 168 , no. 4 , pp. 344-351 . https://doi.org/10.1016/j.jplph.2010.07.019 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.jplph.2010.07.019 |
container_title |
Journal of Plant Physiology |
container_volume |
168 |
container_issue |
4 |
container_start_page |
344 |
op_container_end_page |
351 |
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1766145792704249856 |